Toxoplasma gondii in four captive kangaroos (Macropus spp.) in China: Isolation of a strain of a new genotype from an eastern grey kangaroo (Macropus giganteus)
Marsupials are highly susceptible to Toxoplasma gondii infection. Here, we report T. gondii infection in four kangaroos from a zoo in China. Kangaroos were imported into China in 2000 and were since bred in zoo. In 2017–2018, four kangaroos died due to respiratory system disease or injury. The bodie...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-04-01
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| Series: | International Journal for Parasitology: Parasites and Wildlife |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213224419300161 |
| _version_ | 1828941409175994368 |
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| author | Ruijing Su Hui Dong Tongyi Li Yibao Jiang Ziguo Yuan Chunlei Su Longxian Zhang Yurong Yang |
| author_facet | Ruijing Su Hui Dong Tongyi Li Yibao Jiang Ziguo Yuan Chunlei Su Longxian Zhang Yurong Yang |
| author_sort | Ruijing Su |
| collection | DOAJ |
| description | Marsupials are highly susceptible to Toxoplasma gondii infection. Here, we report T. gondii infection in four kangaroos from a zoo in China. Kangaroos were imported into China in 2000 and were since bred in zoo. In 2017–2018, four kangaroos died due to respiratory system disease or injury. The bodies were submitted to the laboratory to test for T. gondii infection. Antibodies to T. gondii were found in 75% (3/4) of the kangaroos via the modified agglutination test with the cut-off 1:25. Cysts were observed in the histopathological sections of tongue and diaphragm or squashes of fresh myocardium in two kangaroos. These cysts were confirmed as T. gondii by immunohistochemical staining and molecular biological analysis. One viable T. gondii strain was isolated from one kangaroo and designated as TgRooCHn1. DNA from T. gondii tachyzoites obtained from cell culture was characterized by 10 PCR-RFLP markers and the virulence genes ROP5 and ROP18. The genotype of this isolate did not match with any known genotypes; it was designated as ToxoDB#292. The virulence of TgRooCHn1 (104 tachyzoites) was non-lethal to mice, and it formed tissue cysts. To our knowledge, the present study is the first isolation of ToxoDB#292 strain from kangaroo. Improvemets for captive settings were initiated, including greater attention being paied to birds and stray cats, fed frozen meat for carnivores. Keywords: Toxoplasmosis, Kangaroo, Isolation, Genotype, Virulence, China |
| first_indexed | 2024-12-14T03:36:09Z |
| format | Article |
| id | doaj.art-94e88204eebf462588998be77aa99975 |
| institution | Directory Open Access Journal |
| issn | 2213-2244 |
| language | English |
| last_indexed | 2024-12-14T03:36:09Z |
| publishDate | 2019-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal for Parasitology: Parasites and Wildlife |
| spelling | doaj.art-94e88204eebf462588998be77aa999752022-12-21T23:18:37ZengElsevierInternational Journal for Parasitology: Parasites and Wildlife2213-22442019-04-018234239Toxoplasma gondii in four captive kangaroos (Macropus spp.) in China: Isolation of a strain of a new genotype from an eastern grey kangaroo (Macropus giganteus)Ruijing Su0Hui Dong1Tongyi Li2Yibao Jiang3Ziguo Yuan4Chunlei Su5Longxian Zhang6Yurong Yang7College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, PR ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, PR ChinaZhengzhou Zoo, Zhengzhou, PR ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, PR ChinaKey Laboratory of Zoonosis of Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou, PR ChinaDepartment of Microbiology, University of Tennessee, Knoxville, USACollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, PR ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, PR China; Corresponding author.Marsupials are highly susceptible to Toxoplasma gondii infection. Here, we report T. gondii infection in four kangaroos from a zoo in China. Kangaroos were imported into China in 2000 and were since bred in zoo. In 2017–2018, four kangaroos died due to respiratory system disease or injury. The bodies were submitted to the laboratory to test for T. gondii infection. Antibodies to T. gondii were found in 75% (3/4) of the kangaroos via the modified agglutination test with the cut-off 1:25. Cysts were observed in the histopathological sections of tongue and diaphragm or squashes of fresh myocardium in two kangaroos. These cysts were confirmed as T. gondii by immunohistochemical staining and molecular biological analysis. One viable T. gondii strain was isolated from one kangaroo and designated as TgRooCHn1. DNA from T. gondii tachyzoites obtained from cell culture was characterized by 10 PCR-RFLP markers and the virulence genes ROP5 and ROP18. The genotype of this isolate did not match with any known genotypes; it was designated as ToxoDB#292. The virulence of TgRooCHn1 (104 tachyzoites) was non-lethal to mice, and it formed tissue cysts. To our knowledge, the present study is the first isolation of ToxoDB#292 strain from kangaroo. Improvemets for captive settings were initiated, including greater attention being paied to birds and stray cats, fed frozen meat for carnivores. Keywords: Toxoplasmosis, Kangaroo, Isolation, Genotype, Virulence, Chinahttp://www.sciencedirect.com/science/article/pii/S2213224419300161 |
| spellingShingle | Ruijing Su Hui Dong Tongyi Li Yibao Jiang Ziguo Yuan Chunlei Su Longxian Zhang Yurong Yang Toxoplasma gondii in four captive kangaroos (Macropus spp.) in China: Isolation of a strain of a new genotype from an eastern grey kangaroo (Macropus giganteus) International Journal for Parasitology: Parasites and Wildlife |
| title | Toxoplasma gondii in four captive kangaroos (Macropus spp.) in China: Isolation of a strain of a new genotype from an eastern grey kangaroo (Macropus giganteus) |
| title_full | Toxoplasma gondii in four captive kangaroos (Macropus spp.) in China: Isolation of a strain of a new genotype from an eastern grey kangaroo (Macropus giganteus) |
| title_fullStr | Toxoplasma gondii in four captive kangaroos (Macropus spp.) in China: Isolation of a strain of a new genotype from an eastern grey kangaroo (Macropus giganteus) |
| title_full_unstemmed | Toxoplasma gondii in four captive kangaroos (Macropus spp.) in China: Isolation of a strain of a new genotype from an eastern grey kangaroo (Macropus giganteus) |
| title_short | Toxoplasma gondii in four captive kangaroos (Macropus spp.) in China: Isolation of a strain of a new genotype from an eastern grey kangaroo (Macropus giganteus) |
| title_sort | toxoplasma gondii in four captive kangaroos macropus spp in china isolation of a strain of a new genotype from an eastern grey kangaroo macropus giganteus |
| url | http://www.sciencedirect.com/science/article/pii/S2213224419300161 |
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