Nascent high density lipoproteins from liver perfusates of orotic acid-fed rats.
Uniformly fatty livers from orotic acid-fed rats secreted almost no very low density lipoproteins (VLDL) but normal amounts of nascent high density lipoproteins (HDL) accumulated in perfusates. When lecithin:cholesterol acyltransferase (LCAT) was inhibited, nascent HDL were uniformly discoidal and l...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1990-04-01
|
| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520387721 |
| _version_ | 1819058203066892288 |
|---|---|
| author | R L Hamilton L S Guo T E Felker Y S Chao R J Havel |
| author_facet | R L Hamilton L S Guo T E Felker Y S Chao R J Havel |
| author_sort | R L Hamilton |
| collection | DOAJ |
| description | Uniformly fatty livers from orotic acid-fed rats secreted almost no very low density lipoproteins (VLDL) but normal amounts of nascent high density lipoproteins (HDL) accumulated in perfusates. When lecithin:cholesterol acyltransferase (LCAT) was inhibited, nascent HDL were uniformly discoidal and lacked cholesteryl esters. Lipid and apoprotein compositions of nascent HDL from normal and fatty livers were similar whether LCAT was inhibited or not. Apolipoprotein B-100 was not detected in perfusates of uniformly fatty livers, but small amounts of apolipoprotein B-48 were present in HDL2 fractions. Nascent lipoproteins were not seen in Golgi compartments, but lipid-rich particles were clearly evident in endoplasmic reticulum cisternae adjacent to the cis face of the Golgi complex, suggesting that orotic acid blocks VLDL secretion by preventing translocation of nascent particles from the endoplasmic reticulum to the cis Golgi compartment. The accumulation of normal amounts of discoidal HDL in liver perfusates despite virtual absence of triglyceride-rich lipoproteins in Golgi secretory compartments, the space of Disse, and the perfusate is inconsistent with the concept that nascent HDL are exclusively a product of surface remnants cast off during lipolysis of chylomicrons and VLDL. |
| first_indexed | 2024-12-21T13:51:28Z |
| format | Article |
| id | doaj.art-94e91f97822f49559e60aaeefc471859 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-21T13:51:28Z |
| publishDate | 1990-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-94e91f97822f49559e60aaeefc4718592022-12-21T19:01:40ZengElsevierJournal of Lipid Research0022-22751990-04-01279967978Nascent high density lipoproteins from liver perfusates of orotic acid-fed rats.R L HamiltonL S GuoT E FelkerY S ChaoR J HavelUniformly fatty livers from orotic acid-fed rats secreted almost no very low density lipoproteins (VLDL) but normal amounts of nascent high density lipoproteins (HDL) accumulated in perfusates. When lecithin:cholesterol acyltransferase (LCAT) was inhibited, nascent HDL were uniformly discoidal and lacked cholesteryl esters. Lipid and apoprotein compositions of nascent HDL from normal and fatty livers were similar whether LCAT was inhibited or not. Apolipoprotein B-100 was not detected in perfusates of uniformly fatty livers, but small amounts of apolipoprotein B-48 were present in HDL2 fractions. Nascent lipoproteins were not seen in Golgi compartments, but lipid-rich particles were clearly evident in endoplasmic reticulum cisternae adjacent to the cis face of the Golgi complex, suggesting that orotic acid blocks VLDL secretion by preventing translocation of nascent particles from the endoplasmic reticulum to the cis Golgi compartment. The accumulation of normal amounts of discoidal HDL in liver perfusates despite virtual absence of triglyceride-rich lipoproteins in Golgi secretory compartments, the space of Disse, and the perfusate is inconsistent with the concept that nascent HDL are exclusively a product of surface remnants cast off during lipolysis of chylomicrons and VLDL.http://www.sciencedirect.com/science/article/pii/S0022227520387721 |
| spellingShingle | R L Hamilton L S Guo T E Felker Y S Chao R J Havel Nascent high density lipoproteins from liver perfusates of orotic acid-fed rats. Journal of Lipid Research |
| title | Nascent high density lipoproteins from liver perfusates of orotic acid-fed rats. |
| title_full | Nascent high density lipoproteins from liver perfusates of orotic acid-fed rats. |
| title_fullStr | Nascent high density lipoproteins from liver perfusates of orotic acid-fed rats. |
| title_full_unstemmed | Nascent high density lipoproteins from liver perfusates of orotic acid-fed rats. |
| title_short | Nascent high density lipoproteins from liver perfusates of orotic acid-fed rats. |
| title_sort | nascent high density lipoproteins from liver perfusates of orotic acid fed rats |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520387721 |
| work_keys_str_mv | AT rlhamilton nascenthighdensitylipoproteinsfromliverperfusatesoforoticacidfedrats AT lsguo nascenthighdensitylipoproteinsfromliverperfusatesoforoticacidfedrats AT tefelker nascenthighdensitylipoproteinsfromliverperfusatesoforoticacidfedrats AT yschao nascenthighdensitylipoproteinsfromliverperfusatesoforoticacidfedrats AT rjhavel nascenthighdensitylipoproteinsfromliverperfusatesoforoticacidfedrats |