Identification of Inhibitors in Lignocellulosic Slurries and Determination of Their Effect on Hydrocarbon-Producing Microorganisms
The aim of this work was to identify inhibitors in pretreated lignocellulosic slurries, evaluate high-throughput screening strategies, and investigate the impact of inhibitors on potential hydrocarbon-producing microorganisms. Compounds present in slurries that could inhibit microbial growth were id...
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| Format: | Article |
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Frontiers Media S.A.
2018-04-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fbioe.2018.00023/full |
| _version_ | 1819106370998239232 |
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| author | Shihui Yang Shihui Yang Mary Ann Franden Qing Yang Yat-Chen Chou Min Zhang Philip T. Pienkos |
| author_facet | Shihui Yang Shihui Yang Mary Ann Franden Qing Yang Yat-Chen Chou Min Zhang Philip T. Pienkos |
| author_sort | Shihui Yang |
| collection | DOAJ |
| description | The aim of this work was to identify inhibitors in pretreated lignocellulosic slurries, evaluate high-throughput screening strategies, and investigate the impact of inhibitors on potential hydrocarbon-producing microorganisms. Compounds present in slurries that could inhibit microbial growth were identified through a detailed analysis of saccharified slurries by applying a combination of approaches of high-performance liquid chromatography, GC-MS, LC-DAD-MS, and ICP-MS. Several high-throughput assays were then evaluated to generate toxicity profiles. Our results demonstrated that Bioscreen C was useful for analyzing bacterial toxicity but not for yeast. AlamarBlue reduction assay can be a useful high-throughput assay for both bacterial and yeast strains as long as medium components do not interfere with fluorescence measurements. In addition, this work identified two major inhibitors (furfural and ammonium acetate) for three potential hydrocarbon-producing bacterial species that include Escherichia coli, Cupriavidus necator, and Rhodococcus opacus PD630, which are also the primary inhibitors for ethanologens. This study was strived to establish a pipeline to quantify inhibitory compounds in biomass slurries and high-throughput approaches to investigate the effect of inhibitors on microbial biocatalysts, which can be applied for various biomass slurries or hydrolyzates generated through different pretreatment and enzymatic hydrolysis processes or different microbial candidates. |
| first_indexed | 2024-12-22T02:37:04Z |
| format | Article |
| id | doaj.art-94e9345df71e4e8689cfe1ae26b8c01a |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-22T02:37:04Z |
| publishDate | 2018-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-94e9345df71e4e8689cfe1ae26b8c01a2022-12-21T18:41:44ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852018-04-01610.3389/fbioe.2018.00023340358Identification of Inhibitors in Lignocellulosic Slurries and Determination of Their Effect on Hydrocarbon-Producing MicroorganismsShihui Yang0Shihui Yang1Mary Ann Franden2Qing Yang3Yat-Chen Chou4Min Zhang5Philip T. Pienkos6Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Environmental Microbial Technology Center of Hubei Province, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, ChinaNational Bioenergy Center, National Renewable Energy Laboratory, Golden, CO, United StatesNational Bioenergy Center, National Renewable Energy Laboratory, Golden, CO, United StatesHubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Environmental Microbial Technology Center of Hubei Province, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, ChinaNational Bioenergy Center, National Renewable Energy Laboratory, Golden, CO, United StatesNational Bioenergy Center, National Renewable Energy Laboratory, Golden, CO, United StatesNational Bioenergy Center, National Renewable Energy Laboratory, Golden, CO, United StatesThe aim of this work was to identify inhibitors in pretreated lignocellulosic slurries, evaluate high-throughput screening strategies, and investigate the impact of inhibitors on potential hydrocarbon-producing microorganisms. Compounds present in slurries that could inhibit microbial growth were identified through a detailed analysis of saccharified slurries by applying a combination of approaches of high-performance liquid chromatography, GC-MS, LC-DAD-MS, and ICP-MS. Several high-throughput assays were then evaluated to generate toxicity profiles. Our results demonstrated that Bioscreen C was useful for analyzing bacterial toxicity but not for yeast. AlamarBlue reduction assay can be a useful high-throughput assay for both bacterial and yeast strains as long as medium components do not interfere with fluorescence measurements. In addition, this work identified two major inhibitors (furfural and ammonium acetate) for three potential hydrocarbon-producing bacterial species that include Escherichia coli, Cupriavidus necator, and Rhodococcus opacus PD630, which are also the primary inhibitors for ethanologens. This study was strived to establish a pipeline to quantify inhibitory compounds in biomass slurries and high-throughput approaches to investigate the effect of inhibitors on microbial biocatalysts, which can be applied for various biomass slurries or hydrolyzates generated through different pretreatment and enzymatic hydrolysis processes or different microbial candidates.http://journal.frontiersin.org/article/10.3389/fbioe.2018.00023/fullsaccharified slurryinhibitorfurfuralacetatehigh-throughput screening |
| spellingShingle | Shihui Yang Shihui Yang Mary Ann Franden Qing Yang Yat-Chen Chou Min Zhang Philip T. Pienkos Identification of Inhibitors in Lignocellulosic Slurries and Determination of Their Effect on Hydrocarbon-Producing Microorganisms Frontiers in Bioengineering and Biotechnology saccharified slurry inhibitor furfural acetate high-throughput screening |
| title | Identification of Inhibitors in Lignocellulosic Slurries and Determination of Their Effect on Hydrocarbon-Producing Microorganisms |
| title_full | Identification of Inhibitors in Lignocellulosic Slurries and Determination of Their Effect on Hydrocarbon-Producing Microorganisms |
| title_fullStr | Identification of Inhibitors in Lignocellulosic Slurries and Determination of Their Effect on Hydrocarbon-Producing Microorganisms |
| title_full_unstemmed | Identification of Inhibitors in Lignocellulosic Slurries and Determination of Their Effect on Hydrocarbon-Producing Microorganisms |
| title_short | Identification of Inhibitors in Lignocellulosic Slurries and Determination of Their Effect on Hydrocarbon-Producing Microorganisms |
| title_sort | identification of inhibitors in lignocellulosic slurries and determination of their effect on hydrocarbon producing microorganisms |
| topic | saccharified slurry inhibitor furfural acetate high-throughput screening |
| url | http://journal.frontiersin.org/article/10.3389/fbioe.2018.00023/full |
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