Changes in Disease Resistance Phenotypes Associated With Plant Physiological Age Are Not Caused by Variation in R Gene Transcript Abundance
Foliar late blight is one of the most important diseases of potato. Foliar blight resistance has been shown to change as a plant ages. In other pathosystems, resistance (R) gene transcript levels appear to be correlated to disease resistance. The cloning of the broad-spectrum, foliar blight resistan...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2009-03-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-3-0362 |
| _version_ | 1811290152092303360 |
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| author | Benjamin P. Millett Dimitre S. Mollov Massimo Iorizzo Domenico Carputo James M. Bradeen |
| author_facet | Benjamin P. Millett Dimitre S. Mollov Massimo Iorizzo Domenico Carputo James M. Bradeen |
| author_sort | Benjamin P. Millett |
| collection | DOAJ |
| description | Foliar late blight is one of the most important diseases of potato. Foliar blight resistance has been shown to change as a plant ages. In other pathosystems, resistance (R) gene transcript levels appear to be correlated to disease resistance. The cloning of the broad-spectrum, foliar blight resistance gene RB provided the opportunity to explore how foliar blight resistance and R-gene transcript levels vary with plant age. Plants of Solanum bulbocastanum PT29, from which RB, including the native promoter and other flanking regions, was cloned, and S. tuberosum cv. Dark Red Norland (nontransformed and RB-transformed) representing three different developmental stages were screened for resistance to late blight and RB transcript levels. Preflowering plants of all genotypes exhibited the highest levels of resistance, followed by postflowering and near-senescing plants. The RB transgene significantly affected resistance, enhancing resistance levels of all RB-containing lines, especially in younger plants. RB transgene transcripts were detected at all plant ages, despite weak correlation with disease resistance. Consistent transcript levels in plants of different physiological ages with variable levels of disease resistance demonstrate that changes in disease-resistance phenotypes associated with plant age cannot be attributed to changes in R-gene transcript abundance. |
| first_indexed | 2024-04-13T04:07:57Z |
| format | Article |
| id | doaj.art-94ebd786181f49bba266be8ff60f7515 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:07:57Z |
| publishDate | 2009-03-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-94ebd786181f49bba266be8ff60f75152022-12-22T03:03:11ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062009-03-0122336236810.1094/MPMI-22-3-0362Changes in Disease Resistance Phenotypes Associated With Plant Physiological Age Are Not Caused by Variation in R Gene Transcript AbundanceBenjamin P. MillettDimitre S. MollovMassimo IorizzoDomenico CarputoJames M. BradeenFoliar late blight is one of the most important diseases of potato. Foliar blight resistance has been shown to change as a plant ages. In other pathosystems, resistance (R) gene transcript levels appear to be correlated to disease resistance. The cloning of the broad-spectrum, foliar blight resistance gene RB provided the opportunity to explore how foliar blight resistance and R-gene transcript levels vary with plant age. Plants of Solanum bulbocastanum PT29, from which RB, including the native promoter and other flanking regions, was cloned, and S. tuberosum cv. Dark Red Norland (nontransformed and RB-transformed) representing three different developmental stages were screened for resistance to late blight and RB transcript levels. Preflowering plants of all genotypes exhibited the highest levels of resistance, followed by postflowering and near-senescing plants. The RB transgene significantly affected resistance, enhancing resistance levels of all RB-containing lines, especially in younger plants. RB transgene transcripts were detected at all plant ages, despite weak correlation with disease resistance. Consistent transcript levels in plants of different physiological ages with variable levels of disease resistance demonstrate that changes in disease-resistance phenotypes associated with plant age cannot be attributed to changes in R-gene transcript abundance.https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-3-0362 |
| spellingShingle | Benjamin P. Millett Dimitre S. Mollov Massimo Iorizzo Domenico Carputo James M. Bradeen Changes in Disease Resistance Phenotypes Associated With Plant Physiological Age Are Not Caused by Variation in R Gene Transcript Abundance Molecular Plant-Microbe Interactions |
| title | Changes in Disease Resistance Phenotypes Associated With Plant Physiological Age Are Not Caused by Variation in R Gene Transcript Abundance |
| title_full | Changes in Disease Resistance Phenotypes Associated With Plant Physiological Age Are Not Caused by Variation in R Gene Transcript Abundance |
| title_fullStr | Changes in Disease Resistance Phenotypes Associated With Plant Physiological Age Are Not Caused by Variation in R Gene Transcript Abundance |
| title_full_unstemmed | Changes in Disease Resistance Phenotypes Associated With Plant Physiological Age Are Not Caused by Variation in R Gene Transcript Abundance |
| title_short | Changes in Disease Resistance Phenotypes Associated With Plant Physiological Age Are Not Caused by Variation in R Gene Transcript Abundance |
| title_sort | changes in disease resistance phenotypes associated with plant physiological age are not caused by variation in r gene transcript abundance |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-3-0362 |
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