Identification of HSP90 as potential biomarker of biliary atresia using two-dimensional electrophoresis and mass spectrometry.
Biliary atresia (BA) is a devastating cholestatic liver disease targeting infants. Current diagnosis depends on surgical exploration of the biliary tree. The aim of the present study was to identify potential biomarkers for the diagnosis of biliary atresia (BA). Two-dimensional electrophoresis was u...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3708914?pdf=render |
| _version_ | 1819133347548364800 |
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| author | Rui Dong Panmo Deng Yanlei Huang Chun Shen Ping Xue Shan Zheng |
| author_facet | Rui Dong Panmo Deng Yanlei Huang Chun Shen Ping Xue Shan Zheng |
| author_sort | Rui Dong |
| collection | DOAJ |
| description | Biliary atresia (BA) is a devastating cholestatic liver disease targeting infants. Current diagnosis depends on surgical exploration of the biliary tree. The aim of the present study was to identify potential biomarkers for the diagnosis of biliary atresia (BA). Two-dimensional electrophoresis was utilized for the identification of proteins that were differentially expressed in liver biopsies of 20 BA patients and 12 infants with non-BA neonatal cholestasis (NC) as controls. Using mass spectrometry, we identified 15 proteins with expressions significantly altered. Out of the 15 proteins identified, heat shock protein (HSP) 90 was the most significantly altered and was down-regulated in BA samples compared to NC samples using immunoblotting analysis. Our findings suggest that HSP90 might be a potential biomarker for the diagnosis of BA and may be used for monitoring further development and therapy for BA. This study demonstrated that a comprehensive strategy of proteomic identification combined with further validation should be adopted in biomarker discovery. |
| first_indexed | 2024-12-22T09:45:51Z |
| format | Article |
| id | doaj.art-94ee59c4a4ec4b838e692696f3238329 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T09:45:51Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-94ee59c4a4ec4b838e692696f32383292022-12-21T18:30:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0187e6860210.1371/journal.pone.0068602Identification of HSP90 as potential biomarker of biliary atresia using two-dimensional electrophoresis and mass spectrometry.Rui DongPanmo DengYanlei HuangChun ShenPing XueShan ZhengBiliary atresia (BA) is a devastating cholestatic liver disease targeting infants. Current diagnosis depends on surgical exploration of the biliary tree. The aim of the present study was to identify potential biomarkers for the diagnosis of biliary atresia (BA). Two-dimensional electrophoresis was utilized for the identification of proteins that were differentially expressed in liver biopsies of 20 BA patients and 12 infants with non-BA neonatal cholestasis (NC) as controls. Using mass spectrometry, we identified 15 proteins with expressions significantly altered. Out of the 15 proteins identified, heat shock protein (HSP) 90 was the most significantly altered and was down-regulated in BA samples compared to NC samples using immunoblotting analysis. Our findings suggest that HSP90 might be a potential biomarker for the diagnosis of BA and may be used for monitoring further development and therapy for BA. This study demonstrated that a comprehensive strategy of proteomic identification combined with further validation should be adopted in biomarker discovery.http://europepmc.org/articles/PMC3708914?pdf=render |
| spellingShingle | Rui Dong Panmo Deng Yanlei Huang Chun Shen Ping Xue Shan Zheng Identification of HSP90 as potential biomarker of biliary atresia using two-dimensional electrophoresis and mass spectrometry. PLoS ONE |
| title | Identification of HSP90 as potential biomarker of biliary atresia using two-dimensional electrophoresis and mass spectrometry. |
| title_full | Identification of HSP90 as potential biomarker of biliary atresia using two-dimensional electrophoresis and mass spectrometry. |
| title_fullStr | Identification of HSP90 as potential biomarker of biliary atresia using two-dimensional electrophoresis and mass spectrometry. |
| title_full_unstemmed | Identification of HSP90 as potential biomarker of biliary atresia using two-dimensional electrophoresis and mass spectrometry. |
| title_short | Identification of HSP90 as potential biomarker of biliary atresia using two-dimensional electrophoresis and mass spectrometry. |
| title_sort | identification of hsp90 as potential biomarker of biliary atresia using two dimensional electrophoresis and mass spectrometry |
| url | http://europepmc.org/articles/PMC3708914?pdf=render |
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