Colistin-resistant Escherichia coli with mcr genes in the livestock of rural small-scale farms in Ecuador
Abstract Objective Emergence and dissemination of colistin-resistant (Co-R) bacteria harboring mobile colistin resistance genes pose a threat for treatment of infections caused by multi-drug resistant bacteria. Although the worldwide spread of Co-R bacteria is known, the precise state of Co-R bacter...
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| Format: | Article |
| Language: | English |
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BMC
2019-03-01
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| Series: | BMC Research Notes |
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| Online Access: | http://link.springer.com/article/10.1186/s13104-019-4144-0 |
| _version_ | 1798043068303147008 |
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| author | Yoshimasa Yamamoto Manuel Calvopina Ricardo Izurieta Irina Villacres Ryuji Kawahara Masahiro Sasaki Mayumi Yamamoto |
| author_facet | Yoshimasa Yamamoto Manuel Calvopina Ricardo Izurieta Irina Villacres Ryuji Kawahara Masahiro Sasaki Mayumi Yamamoto |
| author_sort | Yoshimasa Yamamoto |
| collection | DOAJ |
| description | Abstract Objective Emergence and dissemination of colistin-resistant (Co-R) bacteria harboring mobile colistin resistance genes pose a threat for treatment of infections caused by multi-drug resistant bacteria. Although the worldwide spread of Co-R bacteria is known, the precise state of Co-R bacterial dissemination in livestock of Andean countries remains unclear. Therefore, we investigated mcr-containing Co-R Escherichia coli dissemination in livestock on small-scale farms in two socioecologically different regions of Ecuador: the Amazonian rain-forest and the Pacific Coast. Results Sixty-six rectal swab samples from 34 pigs and 32 chickens, from five farms in the two regions, were assessed for the dissemination of Co-R E. coli using the selective medium CHROMagar™ COL-APSE. mcr-containing Co-R E. coli were detected in the specimens at a high rate (47%; 31/66), but the detection rates of the two regions were not statistically different. Both chickens and pigs showed similar detection rates. All Co-R E. coli isolates harbored mcr-1. The minimum inhibitory concentrations of colistin were ≥ 8 mg/L, and 67.7% (21/31) of the Co-R isolates were multi-drug resistant. Pulsed-field gel electrophoresis revealed the limited relation between isolates. Thus, we revealed the high rate of widespread dissemination of Co-R bacteria in livestock regardless of the socioecological conditions in Ecuador. |
| first_indexed | 2024-04-11T22:44:27Z |
| format | Article |
| id | doaj.art-94f50a7366f140aa9e09c972365677e7 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-04-11T22:44:27Z |
| publishDate | 2019-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-94f50a7366f140aa9e09c972365677e72022-12-22T03:58:51ZengBMCBMC Research Notes1756-05002019-03-011211510.1186/s13104-019-4144-0Colistin-resistant Escherichia coli with mcr genes in the livestock of rural small-scale farms in EcuadorYoshimasa Yamamoto0Manuel Calvopina1Ricardo Izurieta2Irina Villacres3Ryuji Kawahara4Masahiro Sasaki5Mayumi Yamamoto6Graduate School of Pharmaceutical Sciences, Osaka UniversityUniversidad De Las AmericasCollege of Public Health, University of South FloridaUniversidad De Las AmericasOsaka Institute of Public HealthGraduate School of Pharmaceutical Sciences, Osaka UniversityHealth Administration Center, Gifu UniversityAbstract Objective Emergence and dissemination of colistin-resistant (Co-R) bacteria harboring mobile colistin resistance genes pose a threat for treatment of infections caused by multi-drug resistant bacteria. Although the worldwide spread of Co-R bacteria is known, the precise state of Co-R bacterial dissemination in livestock of Andean countries remains unclear. Therefore, we investigated mcr-containing Co-R Escherichia coli dissemination in livestock on small-scale farms in two socioecologically different regions of Ecuador: the Amazonian rain-forest and the Pacific Coast. Results Sixty-six rectal swab samples from 34 pigs and 32 chickens, from five farms in the two regions, were assessed for the dissemination of Co-R E. coli using the selective medium CHROMagar™ COL-APSE. mcr-containing Co-R E. coli were detected in the specimens at a high rate (47%; 31/66), but the detection rates of the two regions were not statistically different. Both chickens and pigs showed similar detection rates. All Co-R E. coli isolates harbored mcr-1. The minimum inhibitory concentrations of colistin were ≥ 8 mg/L, and 67.7% (21/31) of the Co-R isolates were multi-drug resistant. Pulsed-field gel electrophoresis revealed the limited relation between isolates. Thus, we revealed the high rate of widespread dissemination of Co-R bacteria in livestock regardless of the socioecological conditions in Ecuador.http://link.springer.com/article/10.1186/s13104-019-4144-0Colistin-resistant bacteriamcrLivestockEscherichia coliEcuador |
| spellingShingle | Yoshimasa Yamamoto Manuel Calvopina Ricardo Izurieta Irina Villacres Ryuji Kawahara Masahiro Sasaki Mayumi Yamamoto Colistin-resistant Escherichia coli with mcr genes in the livestock of rural small-scale farms in Ecuador BMC Research Notes Colistin-resistant bacteria mcr Livestock Escherichia coli Ecuador |
| title | Colistin-resistant Escherichia coli with mcr genes in the livestock of rural small-scale farms in Ecuador |
| title_full | Colistin-resistant Escherichia coli with mcr genes in the livestock of rural small-scale farms in Ecuador |
| title_fullStr | Colistin-resistant Escherichia coli with mcr genes in the livestock of rural small-scale farms in Ecuador |
| title_full_unstemmed | Colistin-resistant Escherichia coli with mcr genes in the livestock of rural small-scale farms in Ecuador |
| title_short | Colistin-resistant Escherichia coli with mcr genes in the livestock of rural small-scale farms in Ecuador |
| title_sort | colistin resistant escherichia coli with mcr genes in the livestock of rural small scale farms in ecuador |
| topic | Colistin-resistant bacteria mcr Livestock Escherichia coli Ecuador |
| url | http://link.springer.com/article/10.1186/s13104-019-4144-0 |
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