| Summary: | <b>Background:</b> Crohn’s disease (CD) and ulcerative colitis (UC) are well-defined phenotypes of chronic inflammatory bowel diseases (IBDs). A mechanism of inflammation in these diseases is partially controlled by the intestinal dendritic cell (DC). In this study, we observed a mature CD83<sup>+</sup> DC in colonic bioptic samples, and its correlation with disease phenotype and activity. <b>Methods:</b> The study included 219 subjects: 100 with UC, 44 with CD and 75 healthy subjects. Colonic biopsy specimens were incubated with the primary antibody Anti-CD83. Intraepithelial CD83<sup>+</sup> DCs were counted per 100 enterocytes. The presence of CD83<sup>+</sup> DC was analysed according to the type of IBD, histopathologic inflammation activity and treatment outcome. <b>Results:</b> The presence of mature CD83<sup>+</sup> DCs (0, ≥1) differed according to disease types of IBD (<i>p</i> = 0.001), histologic inflammation activity (<i>p</i> = 0.049) and applied therapy (<i>p</i> = 0.001). The odds for CD83<sup>+</sup> DC presence were 5.2 times higher in the CD group than in the control/UC group. The odds for CD83<sup>+</sup> DC presence were 2.6 times higher in subjects without inflammation or chronic inflammation than with acute inflammation. They were also 3.7 times higher in subjects without therapy. The cut-off value 0.5 CD83<sup>+</sup> DC (Rock analysis area = 0.699; SE 0.046; <i>p</i> < 0.001; 95% CI: 0.609–0.788) had been assessed as a differentiation marker between UC and CD. <b>Conclusion:</b> Presence of CD83<sup>+</sup> DC could be used as a possible parameter in distinction between UC and CD, as well as a predictor of inflammation activity and treatment outcome.
|
|---|