Wnt/β-Catenin Signaling Triggers Neuron Reprogramming and Regeneration in the Mouse Retina
Cell-fusion-mediated somatic-cell reprogramming can be induced in culture; however, whether this process occurs in mammalian tissues remains enigmatic. Here, we show that upon activation of Wnt/β-catenin signaling, mouse retinal neurons can be transiently reprogrammed in vivo back to a precursor sta...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2013-07-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124713002933 |
| _version_ | 1819140421095260160 |
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| author | Daniela Sanges Neus Romo Giacoma Simonte Umberto Di Vicino Ariadna Diaz Tahoces Eduardo Fernández Maria Pia Cosma |
| author_facet | Daniela Sanges Neus Romo Giacoma Simonte Umberto Di Vicino Ariadna Diaz Tahoces Eduardo Fernández Maria Pia Cosma |
| author_sort | Daniela Sanges |
| collection | DOAJ |
| description | Cell-fusion-mediated somatic-cell reprogramming can be induced in culture; however, whether this process occurs in mammalian tissues remains enigmatic. Here, we show that upon activation of Wnt/β-catenin signaling, mouse retinal neurons can be transiently reprogrammed in vivo back to a precursor stage. This occurs after their spontaneous fusion with transplanted hematopoietic stem and progenitor cells (HSPCs). Moreover, we demonstrate that retinal damage is essential for cell-hybrid formation in vivo. Newly formed hybrids can proliferate, commit to differentiation toward a neuroectodermal lineage, and finally develop into terminally differentiated neurons. This results in partial regeneration of the damaged retinal tissue, with functional rescue. Following retinal damage and induction of Wnt/β-catenin signaling, cell-fusion-mediated reprogramming also occurs after endogenous recruitment of bone-marrow-derived cells in the eyes. Our data demonstrate that in vivo reprogramming of terminally differentiated retinal neurons after their fusion with HSPCs is a potential mechanism for tissue regeneration. |
| first_indexed | 2024-12-22T11:38:17Z |
| format | Article |
| id | doaj.art-94fbb341dd184b21aca0ea2bf56d6c58 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-22T11:38:17Z |
| publishDate | 2013-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-94fbb341dd184b21aca0ea2bf56d6c582022-12-21T18:27:22ZengElsevierCell Reports2211-12472013-07-014227128610.1016/j.celrep.2013.06.015Wnt/β-Catenin Signaling Triggers Neuron Reprogramming and Regeneration in the Mouse RetinaDaniela Sanges0Neus Romo1Giacoma Simonte2Umberto Di Vicino3Ariadna Diaz Tahoces4Eduardo Fernández5Maria Pia Cosma6Center for Genomic Regulation (CRG), 08003 Barcelona, SpainCenter for Genomic Regulation (CRG), 08003 Barcelona, SpainCenter for Genomic Regulation (CRG), 08003 Barcelona, SpainCenter for Genomic Regulation (CRG), 08003 Barcelona, SpainBioengineering Institute, University Miguel Hernández and CIBER BBN, 03202 Elche (Alicante), SpainBioengineering Institute, University Miguel Hernández and CIBER BBN, 03202 Elche (Alicante), SpainCenter for Genomic Regulation (CRG), 08003 Barcelona, SpainCell-fusion-mediated somatic-cell reprogramming can be induced in culture; however, whether this process occurs in mammalian tissues remains enigmatic. Here, we show that upon activation of Wnt/β-catenin signaling, mouse retinal neurons can be transiently reprogrammed in vivo back to a precursor stage. This occurs after their spontaneous fusion with transplanted hematopoietic stem and progenitor cells (HSPCs). Moreover, we demonstrate that retinal damage is essential for cell-hybrid formation in vivo. Newly formed hybrids can proliferate, commit to differentiation toward a neuroectodermal lineage, and finally develop into terminally differentiated neurons. This results in partial regeneration of the damaged retinal tissue, with functional rescue. Following retinal damage and induction of Wnt/β-catenin signaling, cell-fusion-mediated reprogramming also occurs after endogenous recruitment of bone-marrow-derived cells in the eyes. Our data demonstrate that in vivo reprogramming of terminally differentiated retinal neurons after their fusion with HSPCs is a potential mechanism for tissue regeneration.http://www.sciencedirect.com/science/article/pii/S2211124713002933 |
| spellingShingle | Daniela Sanges Neus Romo Giacoma Simonte Umberto Di Vicino Ariadna Diaz Tahoces Eduardo Fernández Maria Pia Cosma Wnt/β-Catenin Signaling Triggers Neuron Reprogramming and Regeneration in the Mouse Retina Cell Reports |
| title | Wnt/β-Catenin Signaling Triggers Neuron Reprogramming and Regeneration in the Mouse Retina |
| title_full | Wnt/β-Catenin Signaling Triggers Neuron Reprogramming and Regeneration in the Mouse Retina |
| title_fullStr | Wnt/β-Catenin Signaling Triggers Neuron Reprogramming and Regeneration in the Mouse Retina |
| title_full_unstemmed | Wnt/β-Catenin Signaling Triggers Neuron Reprogramming and Regeneration in the Mouse Retina |
| title_short | Wnt/β-Catenin Signaling Triggers Neuron Reprogramming and Regeneration in the Mouse Retina |
| title_sort | wnt β catenin signaling triggers neuron reprogramming and regeneration in the mouse retina |
| url | http://www.sciencedirect.com/science/article/pii/S2211124713002933 |
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