Multimodal In Vivo Imaging of Retinal and Choroidal Vascular Occlusion
Photoacoustic microscopy (PAM) is an emerging retinal imaging technique that can provide high spatial resolution and high contrast of chorioretinal vessels. PAM is compatible with optical coherence tomography (OCT) and fluorescence imaging, allowing for development of a multimodal imaging system tha...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Photonics |
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| Online Access: | https://www.mdpi.com/2304-6732/9/3/201 |
| _version_ | 1797443507305054208 |
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| author | Van Phuc Nguyen Tianye Zhu Jessica Henry Wei Zhang Xueding Wang Yannis M. Paulus |
| author_facet | Van Phuc Nguyen Tianye Zhu Jessica Henry Wei Zhang Xueding Wang Yannis M. Paulus |
| author_sort | Van Phuc Nguyen |
| collection | DOAJ |
| description | Photoacoustic microscopy (PAM) is an emerging retinal imaging technique that can provide high spatial resolution and high contrast of chorioretinal vessels. PAM is compatible with optical coherence tomography (OCT) and fluorescence imaging, allowing for development of a multimodal imaging system that combines these imaging modalities into one. This study presents a non-invasive, label-free in vivo imaging of retinal and choroidal vascular occlusion using multimodal imaging system, including PAM and OCT. Both retinal vein occlusion (RVO) and choroidal vascular occlusion (CVO) were clearly identified selectively using a spectroscopic PAM imaging. RVO and CVO were created in six rabbits using laser photocoagulation. The dynamic changes of retinal vasculature were observed and evaluated using color fundus photography, fluorescein angiography, OCT, and PAM. The position of RVO and CVO were imaged with different wavelengths ranging from 532 to 600 nm. The data shows that occluded vessels were clearly distinguished from the surrounding retinal vessels on the PAM images. This advanced imaging system is a promising technique for imaging retinal ischemia in preclinical disease models. |
| first_indexed | 2024-03-09T12:57:04Z |
| format | Article |
| id | doaj.art-94fd12f61e3041658371c1b3e2b613a2 |
| institution | Directory Open Access Journal |
| issn | 2304-6732 |
| language | English |
| last_indexed | 2024-03-09T12:57:04Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Photonics |
| spelling | doaj.art-94fd12f61e3041658371c1b3e2b613a22023-11-30T21:59:36ZengMDPI AGPhotonics2304-67322022-03-019320110.3390/photonics9030201Multimodal In Vivo Imaging of Retinal and Choroidal Vascular OcclusionVan Phuc Nguyen0Tianye Zhu1Jessica Henry2Wei Zhang3Xueding Wang4Yannis M. Paulus5Department of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI 48105, USADepartment of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI 48105, USADepartment of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI 48105, USADepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48105, USADepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48105, USADepartment of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI 48105, USAPhotoacoustic microscopy (PAM) is an emerging retinal imaging technique that can provide high spatial resolution and high contrast of chorioretinal vessels. PAM is compatible with optical coherence tomography (OCT) and fluorescence imaging, allowing for development of a multimodal imaging system that combines these imaging modalities into one. This study presents a non-invasive, label-free in vivo imaging of retinal and choroidal vascular occlusion using multimodal imaging system, including PAM and OCT. Both retinal vein occlusion (RVO) and choroidal vascular occlusion (CVO) were clearly identified selectively using a spectroscopic PAM imaging. RVO and CVO were created in six rabbits using laser photocoagulation. The dynamic changes of retinal vasculature were observed and evaluated using color fundus photography, fluorescein angiography, OCT, and PAM. The position of RVO and CVO were imaged with different wavelengths ranging from 532 to 600 nm. The data shows that occluded vessels were clearly distinguished from the surrounding retinal vessels on the PAM images. This advanced imaging system is a promising technique for imaging retinal ischemia in preclinical disease models.https://www.mdpi.com/2304-6732/9/3/201photoacoustic microscopyPAMoptical coherence tomographyOCTretinal vein occlusionchoroidal vascular occlusion |
| spellingShingle | Van Phuc Nguyen Tianye Zhu Jessica Henry Wei Zhang Xueding Wang Yannis M. Paulus Multimodal In Vivo Imaging of Retinal and Choroidal Vascular Occlusion Photonics photoacoustic microscopy PAM optical coherence tomography OCT retinal vein occlusion choroidal vascular occlusion |
| title | Multimodal In Vivo Imaging of Retinal and Choroidal Vascular Occlusion |
| title_full | Multimodal In Vivo Imaging of Retinal and Choroidal Vascular Occlusion |
| title_fullStr | Multimodal In Vivo Imaging of Retinal and Choroidal Vascular Occlusion |
| title_full_unstemmed | Multimodal In Vivo Imaging of Retinal and Choroidal Vascular Occlusion |
| title_short | Multimodal In Vivo Imaging of Retinal and Choroidal Vascular Occlusion |
| title_sort | multimodal in vivo imaging of retinal and choroidal vascular occlusion |
| topic | photoacoustic microscopy PAM optical coherence tomography OCT retinal vein occlusion choroidal vascular occlusion |
| url | https://www.mdpi.com/2304-6732/9/3/201 |
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