THE EFFECTS OF NERVE GROWTH FACTOR ON MYELINATION OF REGENERATED FIBERS IN RAT
The effect of nerve growth factor (NGF) on regeneration of rat sciatic nerves in adult rat was studied. The sciatic nerve was cut out across a 6‑mm gap, then the proximal and distal stumps were inserted into the silicone tube chamber. 7s NGF was extracted from submaxillary gland and th...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2003-07-01
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| Series: | Acta Medica Iranica |
| Subjects: | |
| Online Access: | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/61.pdf&manuscript_id=61 |
| _version_ | 1811296387060465664 |
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| author | M. Firouzi |
| author_facet | M. Firouzi |
| author_sort | M. Firouzi |
| collection | DOAJ |
| description | The effect of nerve growth factor (NGF) on regeneration of rat sciatic nerves in adult rat was studied. The sciatic nerve was cut out across a 6‑mm gap, then the proximal and distal stumps were inserted into the silicone tube chamber. 7s NGF was extracted from submaxillary gland and then was injected into the silicone in experimental group. After seven months nerve was transected and stained with toluidine blue. Semithin sections (1 µm from middle of silicone (control group, without NGF) showed that regenerated axons (mostly unmyelinated) were dispersed randomly, and they were not grouped into bundles. In this group some of the myelinated fibers were degenerated and macrophages or in other word, schwann cells contained a large amount of these degenerated sheaths. Semithin section of experimental group (with NGF) showed numerous regenerated axons (myelinated) that were grouped into small bundles. Schwann cells in experimental group were large and eucromatin and some of them were divided. These data indicate that NGF causes myelinated axons, regenerate and making new myelinated sheaths. |
| first_indexed | 2024-04-13T05:47:56Z |
| format | Article |
| id | doaj.art-95004a5b5c194eb9af6476f871e87835 |
| institution | Directory Open Access Journal |
| issn | 0044-6025 |
| language | English |
| last_indexed | 2024-04-13T05:47:56Z |
| publishDate | 2003-07-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Acta Medica Iranica |
| spelling | doaj.art-95004a5b5c194eb9af6476f871e878352022-12-22T02:59:54ZengTehran University of Medical SciencesActa Medica Iranica0044-60252003-07-01413183187THE EFFECTS OF NERVE GROWTH FACTOR ON MYELINATION OF REGENERATED FIBERS IN RATM. FirouziThe effect of nerve growth factor (NGF) on regeneration of rat sciatic nerves in adult rat was studied. The sciatic nerve was cut out across a 6‑mm gap, then the proximal and distal stumps were inserted into the silicone tube chamber. 7s NGF was extracted from submaxillary gland and then was injected into the silicone in experimental group. After seven months nerve was transected and stained with toluidine blue. Semithin sections (1 µm from middle of silicone (control group, without NGF) showed that regenerated axons (mostly unmyelinated) were dispersed randomly, and they were not grouped into bundles. In this group some of the myelinated fibers were degenerated and macrophages or in other word, schwann cells contained a large amount of these degenerated sheaths. Semithin section of experimental group (with NGF) showed numerous regenerated axons (myelinated) that were grouped into small bundles. Schwann cells in experimental group were large and eucromatin and some of them were divided. These data indicate that NGF causes myelinated axons, regenerate and making new myelinated sheaths.http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/61.pdf&manuscript_id=61Peripheral nerve regenerationschwann cellNGFmyelinationrat |
| spellingShingle | M. Firouzi THE EFFECTS OF NERVE GROWTH FACTOR ON MYELINATION OF REGENERATED FIBERS IN RAT Acta Medica Iranica Peripheral nerve regeneration schwann cell NGF myelination rat |
| title | THE EFFECTS OF NERVE GROWTH FACTOR ON MYELINATION OF REGENERATED FIBERS IN RAT |
| title_full | THE EFFECTS OF NERVE GROWTH FACTOR ON MYELINATION OF REGENERATED FIBERS IN RAT |
| title_fullStr | THE EFFECTS OF NERVE GROWTH FACTOR ON MYELINATION OF REGENERATED FIBERS IN RAT |
| title_full_unstemmed | THE EFFECTS OF NERVE GROWTH FACTOR ON MYELINATION OF REGENERATED FIBERS IN RAT |
| title_short | THE EFFECTS OF NERVE GROWTH FACTOR ON MYELINATION OF REGENERATED FIBERS IN RAT |
| title_sort | effects of nerve growth factor on myelination of regenerated fibers in rat |
| topic | Peripheral nerve regeneration schwann cell NGF myelination rat |
| url | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/61.pdf&manuscript_id=61 |
| work_keys_str_mv | AT mfirouzi theeffectsofnervegrowthfactoronmyelinationofregeneratedfibersinrat AT mfirouzi effectsofnervegrowthfactoronmyelinationofregeneratedfibersinrat |