Differences in neural-immune gene expression response in rat spinal dorsal horn correlates with variations in electroacupuncture analgesia.
BACKGROUND: Electroacupuncture (EA) has been widely used to alleviate diverse pains. Accumulated clinical experiences and experimental observations indicated that significant differences exist in sensitivity to EA analgesia for individuals of patients and model animals. However, the molecular mechan...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3411776?pdf=render |
| _version_ | 1818962579436863488 |
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| author | Ke Wang Rong Zhang Xiaohui Xiang Fei He Libo Lin Xingjie Ping Lei Yu Jisheng Han Guoping Zhao Qinghua Zhang Cailian Cui |
| author_facet | Ke Wang Rong Zhang Xiaohui Xiang Fei He Libo Lin Xingjie Ping Lei Yu Jisheng Han Guoping Zhao Qinghua Zhang Cailian Cui |
| author_sort | Ke Wang |
| collection | DOAJ |
| description | BACKGROUND: Electroacupuncture (EA) has been widely used to alleviate diverse pains. Accumulated clinical experiences and experimental observations indicated that significant differences exist in sensitivity to EA analgesia for individuals of patients and model animals. However, the molecular mechanism accounting for this difference remains obscure. METHODOLOGY/PRINCIPAL FINDINGS: We classified model male rats into high-responder (HR; TFL changes >150) and non-responder (NR; TFL changes ≤ 0) groups based on changes of their pain threshold detected by tail-flick latency (TFL) before and after 2 Hz or 100 Hz EA treatment. Gene expression analysis of spinal dorsal horn (DH) revealed divergent expression in HR and NR after 2 Hz/100 Hz EA. The expression of the neurotransmitter system related genes was significantly highly regulated in the HR animals while the proinflammation cytokines related genes were up-regulated more significantly in NR than that in HR after 2 Hz and 100 Hz EA stimulation, especially in the case of 2 Hz stimulation. CONCLUSIONS/SIGNIFICANCE: Our results suggested that differential regulation and coordination of neural-immune related genes might play an important role for individual variations in analgesic effects responding to EA in DH. It also provided new candidate genes related to EA responsiveness for future investigation. |
| first_indexed | 2024-12-20T12:31:34Z |
| format | Article |
| id | doaj.art-95009d9bd52744159bbc8588624de685 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T12:31:34Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-95009d9bd52744159bbc8588624de6852022-12-21T19:40:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0178e4233110.1371/journal.pone.0042331Differences in neural-immune gene expression response in rat spinal dorsal horn correlates with variations in electroacupuncture analgesia.Ke WangRong ZhangXiaohui XiangFei HeLibo LinXingjie PingLei YuJisheng HanGuoping ZhaoQinghua ZhangCailian CuiBACKGROUND: Electroacupuncture (EA) has been widely used to alleviate diverse pains. Accumulated clinical experiences and experimental observations indicated that significant differences exist in sensitivity to EA analgesia for individuals of patients and model animals. However, the molecular mechanism accounting for this difference remains obscure. METHODOLOGY/PRINCIPAL FINDINGS: We classified model male rats into high-responder (HR; TFL changes >150) and non-responder (NR; TFL changes ≤ 0) groups based on changes of their pain threshold detected by tail-flick latency (TFL) before and after 2 Hz or 100 Hz EA treatment. Gene expression analysis of spinal dorsal horn (DH) revealed divergent expression in HR and NR after 2 Hz/100 Hz EA. The expression of the neurotransmitter system related genes was significantly highly regulated in the HR animals while the proinflammation cytokines related genes were up-regulated more significantly in NR than that in HR after 2 Hz and 100 Hz EA stimulation, especially in the case of 2 Hz stimulation. CONCLUSIONS/SIGNIFICANCE: Our results suggested that differential regulation and coordination of neural-immune related genes might play an important role for individual variations in analgesic effects responding to EA in DH. It also provided new candidate genes related to EA responsiveness for future investigation.http://europepmc.org/articles/PMC3411776?pdf=render |
| spellingShingle | Ke Wang Rong Zhang Xiaohui Xiang Fei He Libo Lin Xingjie Ping Lei Yu Jisheng Han Guoping Zhao Qinghua Zhang Cailian Cui Differences in neural-immune gene expression response in rat spinal dorsal horn correlates with variations in electroacupuncture analgesia. PLoS ONE |
| title | Differences in neural-immune gene expression response in rat spinal dorsal horn correlates with variations in electroacupuncture analgesia. |
| title_full | Differences in neural-immune gene expression response in rat spinal dorsal horn correlates with variations in electroacupuncture analgesia. |
| title_fullStr | Differences in neural-immune gene expression response in rat spinal dorsal horn correlates with variations in electroacupuncture analgesia. |
| title_full_unstemmed | Differences in neural-immune gene expression response in rat spinal dorsal horn correlates with variations in electroacupuncture analgesia. |
| title_short | Differences in neural-immune gene expression response in rat spinal dorsal horn correlates with variations in electroacupuncture analgesia. |
| title_sort | differences in neural immune gene expression response in rat spinal dorsal horn correlates with variations in electroacupuncture analgesia |
| url | http://europepmc.org/articles/PMC3411776?pdf=render |
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