Investigation of gene expression in primary embryonic cell line (FGBC8) from olive flounder Paralichthys olivaceus

In this paper, we have reported the expression of immune-related gene in a new embryonic cell line (FGBC8) which was established from olive flounder (Paralichthys olivaceus) embryos. To explore the cell biotechnological applicability, t...

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Main Authors: Ju-Won Kim, Ja Young Cho, Won-Kyong Chun, Dong-Gyun Kim, Bo-Hye Nam, Eun-Soo Nho, Young-Ok Kim, Hee Jeong Kong
Format: Article
Language:English
Published: The Korean Society of Fisheries and Aquatic Science 2021-11-01
Series:Fisheries and Aquatic Sciences
Subjects:
Online Access:http://www.e-fas.org/archive/view_article?pid=fas-24-11-370
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author Ju-Won Kim
Ja Young Cho
Won-Kyong Chun
Dong-Gyun Kim
Bo-Hye Nam
Eun-Soo Nho
Young-Ok Kim
Hee Jeong Kong
author_facet Ju-Won Kim
Ja Young Cho
Won-Kyong Chun
Dong-Gyun Kim
Bo-Hye Nam
Eun-Soo Nho
Young-Ok Kim
Hee Jeong Kong
author_sort Ju-Won Kim
collection DOAJ
description In this paper, we have reported the expression of immune-related gene in a new embryonic cell line (FGBC8) which was established from olive flounder (Paralichthys olivaceus) embryos. To explore the cell biotechnological applicability, the FGBC8 cells were incubated with the several mitogens such as lipopolysaccharide (LPS), polyinosinic-polycytidylic acid (poly I:C), flagellin,and interferon (IFN)-γ. After incubation, the expression of immune-related gene was observed in FGBC8 cells through the quantitative real-time PCR. Our results indicate that FGBC8 cells will serve as a valuable research tool for investigating host-pathogen interactions as well as cell biotechnological applications.
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spelling doaj.art-9506bbe5b90044368eb34a64bd0ff09e2022-12-22T00:04:45ZengThe Korean Society of Fisheries and Aquatic ScienceFisheries and Aquatic Sciences2234-17572021-11-01241137037410.47853/FAS.2021.e36fas-24-11-370Investigation of gene expression in primary embryonic cell line (FGBC8) from olive flounder Paralichthys olivaceusJu-Won Kim0Ja Young Cho1Won-Kyong Chun2Dong-Gyun Kim3Bo-Hye Nam4Eun-Soo Nho5Young-Ok Kim6Hee Jeong Kong7Biotechnology Research Division, National Institute of Fisheries Science, Biotechnology Research Division, National Institute of Fisheries Science, Biotechnology Research Division, National Institute of Fisheries Science, Biotechnology Research Division, National Institute of Fisheries Science, Biotechnology Research Division, National Institute of Fisheries Science, Biotechnology Research Division, National Institute of Fisheries Science, Biotechnology Research Division, National Institute of Fisheries Science, Biotechnology Research Division, National Institute of Fisheries Science, In this paper, we have reported the expression of immune-related gene in a new embryonic cell line (FGBC8) which was established from olive flounder (Paralichthys olivaceus) embryos. To explore the cell biotechnological applicability, the FGBC8 cells were incubated with the several mitogens such as lipopolysaccharide (LPS), polyinosinic-polycytidylic acid (poly I:C), flagellin,and interferon (IFN)-γ. After incubation, the expression of immune-related gene was observed in FGBC8 cells through the quantitative real-time PCR. Our results indicate that FGBC8 cells will serve as a valuable research tool for investigating host-pathogen interactions as well as cell biotechnological applications.http://www.e-fas.org/archive/view_article?pid=fas-24-11-370olive flounderembryonic cell lineimmune-related gene expression
spellingShingle Ju-Won Kim
Ja Young Cho
Won-Kyong Chun
Dong-Gyun Kim
Bo-Hye Nam
Eun-Soo Nho
Young-Ok Kim
Hee Jeong Kong
Investigation of gene expression in primary embryonic cell line (FGBC8) from olive flounder Paralichthys olivaceus
Fisheries and Aquatic Sciences
olive flounder
embryonic cell line
immune-related gene expression
title Investigation of gene expression in primary embryonic cell line (FGBC8) from olive flounder Paralichthys olivaceus
title_full Investigation of gene expression in primary embryonic cell line (FGBC8) from olive flounder Paralichthys olivaceus
title_fullStr Investigation of gene expression in primary embryonic cell line (FGBC8) from olive flounder Paralichthys olivaceus
title_full_unstemmed Investigation of gene expression in primary embryonic cell line (FGBC8) from olive flounder Paralichthys olivaceus
title_short Investigation of gene expression in primary embryonic cell line (FGBC8) from olive flounder Paralichthys olivaceus
title_sort investigation of gene expression in primary embryonic cell line fgbc8 from olive flounder paralichthys olivaceus
topic olive flounder
embryonic cell line
immune-related gene expression
url http://www.e-fas.org/archive/view_article?pid=fas-24-11-370
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