Protocol for efficient micropropagation of spring gentian and sand jurinea
Protocols for the in vitro propagation of two endangered plant species in the Czech Republic, Gentiana verna L. and Jurinea cyanoides (L.) Rchb., were established. In G. verna, the induction of organogenesis on vegetative shoots was successful on the basal 6% agar WPM medium with 200 mg.l-1of glutam...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2014-01-01
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| Series: | Journal of Forest Science |
| Subjects: | |
| Online Access: | https://jfs.agriculturejournals.cz/artkey/jfs-201401-0002_protocol-for-efficient-micropropagation-of-spring-gentian-and-sand-jurinea.php |
| _version_ | 1797898786462236672 |
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| author | H. Cvrčková P. Máchová J. Dostál J. Malá |
| author_facet | H. Cvrčková P. Máchová J. Dostál J. Malá |
| author_sort | H. Cvrčková |
| collection | DOAJ |
| description | Protocols for the in vitro propagation of two endangered plant species in the Czech Republic, Gentiana verna L. and Jurinea cyanoides (L.) Rchb., were established. In G. verna, the induction of organogenesis on vegetative shoots was successful on the basal 6% agar WPM medium with 200 mg.l-1of glutamine, 200 mg.l-1 of casein hydrolysate, 30 g.l-1 of sucrose, 0.2 mg.l-1 of BAP, and 0.1 mg.l-1 of IBA. The multiplication of primary explants was achieved on 6% agar basal MS medium with the same concentrations of substances mentioned above. In J. cyanoides, the induction of organogenesis and multiplication of its vegetative shoots were successful on MS medium with the same concentration of added substances used in G. verna. On the basal MS medium of 1/3 concentration with 3 mg.l-1 of IBA 70-75% rooting efficiency of G. verna microcuttings and 40% rooting efficiency of J. cyanoides microcuttings were reached. The mortality during acclimatization did not exceed 20% for G. verna and 25% for J. cyanoides. |
| first_indexed | 2024-04-10T08:19:41Z |
| format | Article |
| id | doaj.art-9507c36b9bde4031945b204d8e7309d3 |
| institution | Directory Open Access Journal |
| issn | 1212-4834 1805-935X |
| language | English |
| last_indexed | 2024-04-10T08:19:41Z |
| publishDate | 2014-01-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Journal of Forest Science |
| spelling | doaj.art-9507c36b9bde4031945b204d8e7309d32023-02-23T03:42:32ZengCzech Academy of Agricultural SciencesJournal of Forest Science1212-48341805-935X2014-01-016011510.17221/60/2013-JFSjfs-201401-0002Protocol for efficient micropropagation of spring gentian and sand jurineaH. Cvrčková0P. Máchová1J. Dostál2J. Malá3Forestry and Game Management Research Institute, Jíloviště-Strnady, Czech RepublicForestry and Game Management Research Institute, Jíloviště-Strnady, Czech RepublicForestry and Game Management Research Institute, Jíloviště-Strnady, Czech RepublicForestry and Game Management Research Institute, Jíloviště-Strnady, Czech RepublicProtocols for the in vitro propagation of two endangered plant species in the Czech Republic, Gentiana verna L. and Jurinea cyanoides (L.) Rchb., were established. In G. verna, the induction of organogenesis on vegetative shoots was successful on the basal 6% agar WPM medium with 200 mg.l-1of glutamine, 200 mg.l-1 of casein hydrolysate, 30 g.l-1 of sucrose, 0.2 mg.l-1 of BAP, and 0.1 mg.l-1 of IBA. The multiplication of primary explants was achieved on 6% agar basal MS medium with the same concentrations of substances mentioned above. In J. cyanoides, the induction of organogenesis and multiplication of its vegetative shoots were successful on MS medium with the same concentration of added substances used in G. verna. On the basal MS medium of 1/3 concentration with 3 mg.l-1 of IBA 70-75% rooting efficiency of G. verna microcuttings and 40% rooting efficiency of J. cyanoides microcuttings were reached. The mortality during acclimatization did not exceed 20% for G. verna and 25% for J. cyanoides.https://jfs.agriculturejournals.cz/artkey/jfs-201401-0002_protocol-for-efficient-micropropagation-of-spring-gentian-and-sand-jurinea.phpgentiana vernajurinea cyanoidesin vitro reproductionorganogenesis |
| spellingShingle | H. Cvrčková P. Máchová J. Dostál J. Malá Protocol for efficient micropropagation of spring gentian and sand jurinea Journal of Forest Science gentiana verna jurinea cyanoides in vitro reproduction organogenesis |
| title | Protocol for efficient micropropagation of spring gentian and sand jurinea |
| title_full | Protocol for efficient micropropagation of spring gentian and sand jurinea |
| title_fullStr | Protocol for efficient micropropagation of spring gentian and sand jurinea |
| title_full_unstemmed | Protocol for efficient micropropagation of spring gentian and sand jurinea |
| title_short | Protocol for efficient micropropagation of spring gentian and sand jurinea |
| title_sort | protocol for efficient micropropagation of spring gentian and sand jurinea |
| topic | gentiana verna jurinea cyanoides in vitro reproduction organogenesis |
| url | https://jfs.agriculturejournals.cz/artkey/jfs-201401-0002_protocol-for-efficient-micropropagation-of-spring-gentian-and-sand-jurinea.php |
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