Analysis of molecular markers as IL-12, IL-22 and IFN-γ in correlation with a clinical course in patients with psoriasis
Objectives As a chronic, recurrent, immunologically mediated systemic disease and a common cause of dermatological problems, psoriasis is often a subject of scientific research. Skin changes located on the hands can cause difficulties and limitations in the performance of professional activities, es...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nofer Institute of Occupational Medicine
2020-09-01
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| Series: | International Journal of Occupational Medicine and Environmental Health |
| Subjects: | |
| Online Access: | http://www.journalssystem.com/ijomeh/ANALYSIS-OF-MOLECULAR-MARKERS-AS-IL-12-IL-22-AND-IFN-IN-CORRELATION-WITH-A-CLINICAL,121914,0,2.html |
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| author | Magdalena Kutwin Monika Migdalska-Sęk Ewa Brzeziańska-Lasota Piotr Zelga Anna Woźniacka |
| author_facet | Magdalena Kutwin Monika Migdalska-Sęk Ewa Brzeziańska-Lasota Piotr Zelga Anna Woźniacka |
| author_sort | Magdalena Kutwin |
| collection | DOAJ |
| description | Objectives
As a chronic, recurrent, immunologically mediated systemic disease and a common cause of dermatological problems, psoriasis is often
a subject of scientific research. Skin changes located on the hands can cause difficulties and limitations in the performance of professional activities,
especially manual ones. The main role in pathogenesis is played by immunological factors – improper functioning of the components of the immune
system, among others, T lymphocytes and cytokines like interleukin-12 (IL-12), interleukin-22 (IL-22) and interferon gamma (IFN-γ).
Material and Methods
The obtained tissue and blood were destined for RNA isolation. The RNA was then subjected to a reverse transcription reaction. The relative
gene expression level was evaluated by the real-time polymerase chain reaction for IL-12B , IL-22 and IFN-γ genes, and presented as the relative
quantification (RQ) value, relative to the reference gene GAPDH . In addition, a correlation analysis of the expression level of selected genes with
the clinical course of the disease, as assessed by the Psoriasis Area and Severity Index (PASI), the Body Surface Area (BSA) and the Dermatology Life
Quality Index (DLQI) scores was performed.
Results
Statistical analysis confirmed a significant increase in RQ values for IL-12B , IL-22 and IFN-γ
in the group of psoriatic patients vs. the control group. A positive correlation was also found between BSA and PASI and RQ for the IL-12B gene.
Conclusions
Increased expression levels of IL-12B , IL-22 and IFN-γ genes in psoriatic skin confirm that selected cytokines play an important role in
the initiation and sustenance of psoriasis. Int J Occup Med Environ Health. 2020;33(5):635–47 |
| first_indexed | 2024-12-13T16:09:26Z |
| format | Article |
| id | doaj.art-950b219d72e34708a8464deec565792c |
| institution | Directory Open Access Journal |
| issn | 1232-1087 1896-494X |
| language | English |
| last_indexed | 2024-12-13T16:09:26Z |
| publishDate | 2020-09-01 |
| publisher | Nofer Institute of Occupational Medicine |
| record_format | Article |
| series | International Journal of Occupational Medicine and Environmental Health |
| spelling | doaj.art-950b219d72e34708a8464deec565792c2022-12-21T23:38:58ZengNofer Institute of Occupational MedicineInternational Journal of Occupational Medicine and Environmental Health1232-10871896-494X2020-09-0133563564710.13075/ijomeh.1896.01513121914Analysis of molecular markers as IL-12, IL-22 and IFN-γ in correlation with a clinical course in patients with psoriasisMagdalena Kutwin0Monika Migdalska-Sęk1Ewa Brzeziańska-Lasota2Piotr Zelga3Anna Woźniacka4Medical University of Lodz, Łódź, Poland (Department of Dermatology and Venereology)Medical University of Lodz, Łódź, Poland (Department of Biomedicine and Genetics)Medical University of Lodz, Łódź, Poland (Department of Biomedicine and Genetics)Medical University of Lodz, Łódź, Poland (Department of General and Colorectal Surgery)Medical University of Lodz, Łódź, Poland (Department of Dermatology and Venereology)Objectives As a chronic, recurrent, immunologically mediated systemic disease and a common cause of dermatological problems, psoriasis is often a subject of scientific research. Skin changes located on the hands can cause difficulties and limitations in the performance of professional activities, especially manual ones. The main role in pathogenesis is played by immunological factors – improper functioning of the components of the immune system, among others, T lymphocytes and cytokines like interleukin-12 (IL-12), interleukin-22 (IL-22) and interferon gamma (IFN-γ). Material and Methods The obtained tissue and blood were destined for RNA isolation. The RNA was then subjected to a reverse transcription reaction. The relative gene expression level was evaluated by the real-time polymerase chain reaction for IL-12B , IL-22 and IFN-γ genes, and presented as the relative quantification (RQ) value, relative to the reference gene GAPDH . In addition, a correlation analysis of the expression level of selected genes with the clinical course of the disease, as assessed by the Psoriasis Area and Severity Index (PASI), the Body Surface Area (BSA) and the Dermatology Life Quality Index (DLQI) scores was performed. Results Statistical analysis confirmed a significant increase in RQ values for IL-12B , IL-22 and IFN-γ in the group of psoriatic patients vs. the control group. A positive correlation was also found between BSA and PASI and RQ for the IL-12B gene. Conclusions Increased expression levels of IL-12B , IL-22 and IFN-γ genes in psoriatic skin confirm that selected cytokines play an important role in the initiation and sustenance of psoriasis. Int J Occup Med Environ Health. 2020;33(5):635–47http://www.journalssystem.com/ijomeh/ANALYSIS-OF-MOLECULAR-MARKERS-AS-IL-12-IL-22-AND-IFN-IN-CORRELATION-WITH-A-CLINICAL,121914,0,2.htmlpsoriasisdisease activityproinflammatory cytokinesskin diseaseimmunologymolecular markers |
| spellingShingle | Magdalena Kutwin Monika Migdalska-Sęk Ewa Brzeziańska-Lasota Piotr Zelga Anna Woźniacka Analysis of molecular markers as IL-12, IL-22 and IFN-γ in correlation with a clinical course in patients with psoriasis International Journal of Occupational Medicine and Environmental Health psoriasis disease activity proinflammatory cytokines skin disease immunology molecular markers |
| title | Analysis of molecular markers as IL-12, IL-22 and IFN-γ in correlation with a clinical course
in patients with psoriasis |
| title_full | Analysis of molecular markers as IL-12, IL-22 and IFN-γ in correlation with a clinical course
in patients with psoriasis |
| title_fullStr | Analysis of molecular markers as IL-12, IL-22 and IFN-γ in correlation with a clinical course
in patients with psoriasis |
| title_full_unstemmed | Analysis of molecular markers as IL-12, IL-22 and IFN-γ in correlation with a clinical course
in patients with psoriasis |
| title_short | Analysis of molecular markers as IL-12, IL-22 and IFN-γ in correlation with a clinical course
in patients with psoriasis |
| title_sort | analysis of molecular markers as il 12 il 22 and ifn γ in correlation with a clinical course in patients with psoriasis |
| topic | psoriasis disease activity proinflammatory cytokines skin disease immunology molecular markers |
| url | http://www.journalssystem.com/ijomeh/ANALYSIS-OF-MOLECULAR-MARKERS-AS-IL-12-IL-22-AND-IFN-IN-CORRELATION-WITH-A-CLINICAL,121914,0,2.html |
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