Genome-wide association identifies several QTLs controlling cysteine and methionine content in soybean seed including some promising candidate genes
Abstract Soybean is an important source of protein, oil and carbohydrates, as well as other beneficial nutrients. A major function of proteins in nutrition is to supply adequate amounts of amino acids. Although they are essential for human nutrition, the sulfur-containing amino acids cysteine (Cys)...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-78907-w |
| _version_ | 1818752301686325248 |
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| author | Sidiki Malle Milad Eskandari Malcolm Morrison François Belzile |
| author_facet | Sidiki Malle Milad Eskandari Malcolm Morrison François Belzile |
| author_sort | Sidiki Malle |
| collection | DOAJ |
| description | Abstract Soybean is an important source of protein, oil and carbohydrates, as well as other beneficial nutrients. A major function of proteins in nutrition is to supply adequate amounts of amino acids. Although they are essential for human nutrition, the sulfur-containing amino acids cysteine (Cys) and methionine (Met) are often limited and the genetic control of their content in soybean seeds is poorly characterized. This study aimed to characterize the phenotypic variation and identify quantitative trait loci (QTL) associated with Cys and Met content in a core set of 137 soybean lines, representative of the genetic diversity among Canadian short-season soybean, spanning maturity groups 000-II (MG000-II). Significant phenotypic differences were found among these lines for Cys, Met and Cys + Met content. Using both a mixed linear model and six multi-locus methods with a catalogue of 2.18 M SNPs, we report a total of nine QTLs and seventeen QTNs of which seven comprise promising candidate genes. This work allowed us to reproducibly detect multiple novel loci associated with sulfur-containing amino acid content. The markers and genes identified in this study may be useful for soybean genetic improvement aiming to increase Cys and Met content. |
| first_indexed | 2024-12-18T04:49:18Z |
| format | Article |
| id | doaj.art-951022e99d8e48ea835f61557e848eef |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T04:49:18Z |
| publishDate | 2020-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-951022e99d8e48ea835f61557e848eef2022-12-21T21:20:28ZengNature PortfolioScientific Reports2045-23222020-12-0110111410.1038/s41598-020-78907-wGenome-wide association identifies several QTLs controlling cysteine and methionine content in soybean seed including some promising candidate genesSidiki Malle0Milad Eskandari1Malcolm Morrison2François Belzile3Département de Phytologie, Faculty of Agricultural and Food Sciences and Institute for Integrative and Systems Biology (IBIS), Laval UniversityDepartment of Plant Agriculture, University of GuelphOttawa Research and Development Centre, Agriculture and Agri-Food CanadaDépartement de Phytologie, Faculty of Agricultural and Food Sciences and Institute for Integrative and Systems Biology (IBIS), Laval UniversityAbstract Soybean is an important source of protein, oil and carbohydrates, as well as other beneficial nutrients. A major function of proteins in nutrition is to supply adequate amounts of amino acids. Although they are essential for human nutrition, the sulfur-containing amino acids cysteine (Cys) and methionine (Met) are often limited and the genetic control of their content in soybean seeds is poorly characterized. This study aimed to characterize the phenotypic variation and identify quantitative trait loci (QTL) associated with Cys and Met content in a core set of 137 soybean lines, representative of the genetic diversity among Canadian short-season soybean, spanning maturity groups 000-II (MG000-II). Significant phenotypic differences were found among these lines for Cys, Met and Cys + Met content. Using both a mixed linear model and six multi-locus methods with a catalogue of 2.18 M SNPs, we report a total of nine QTLs and seventeen QTNs of which seven comprise promising candidate genes. This work allowed us to reproducibly detect multiple novel loci associated with sulfur-containing amino acid content. The markers and genes identified in this study may be useful for soybean genetic improvement aiming to increase Cys and Met content.https://doi.org/10.1038/s41598-020-78907-w |
| spellingShingle | Sidiki Malle Milad Eskandari Malcolm Morrison François Belzile Genome-wide association identifies several QTLs controlling cysteine and methionine content in soybean seed including some promising candidate genes Scientific Reports |
| title | Genome-wide association identifies several QTLs controlling cysteine and methionine content in soybean seed including some promising candidate genes |
| title_full | Genome-wide association identifies several QTLs controlling cysteine and methionine content in soybean seed including some promising candidate genes |
| title_fullStr | Genome-wide association identifies several QTLs controlling cysteine and methionine content in soybean seed including some promising candidate genes |
| title_full_unstemmed | Genome-wide association identifies several QTLs controlling cysteine and methionine content in soybean seed including some promising candidate genes |
| title_short | Genome-wide association identifies several QTLs controlling cysteine and methionine content in soybean seed including some promising candidate genes |
| title_sort | genome wide association identifies several qtls controlling cysteine and methionine content in soybean seed including some promising candidate genes |
| url | https://doi.org/10.1038/s41598-020-78907-w |
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