Mechanisms of Proliferative Inhibition by Maimendong & Qianjinweijing Decoction in A549 Cells
Background and objective Traditional Chinese medicine is an approach for malignant tumor treatment with Chinese characteristics. The aim of this study is to investigate the inhibitory effects of Maimendong & qianjinweijing decoction extract on A549 human lung cancer cell line proliferation and e...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | zho |
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Chinese Anti-Cancer Association; Chinese Antituberculosis Association
2010-05-01
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| Series: | Chinese Journal of Lung Cancer |
| Subjects: | |
| Online Access: | http://www.lungca.org/index.php?journal=01&page=article&op=viewFile&path[]=10.3779%2Fj.issn.1009-3419.2010.05.18&path[]=1484 |
| _version_ | 1811255608974770176 |
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| author | Xu ZHANG Jin’ao DUAN Yuping TANG Yuhui ZHOU Zhen ZHAN |
| author_facet | Xu ZHANG Jin’ao DUAN Yuping TANG Yuhui ZHOU Zhen ZHAN |
| author_sort | Xu ZHANG |
| collection | DOAJ |
| description | Background and objective Traditional Chinese medicine is an approach for malignant tumor treatment with Chinese characteristics. The aim of this study is to investigate the inhibitory effects of Maimendong & qianjinweijing decoction extract on A549 human lung cancer cell line proliferation and explored its probable molecular mechanisms. Methods A549 cells were treated with drugs in different does and time. The effects on the proliferation of A549 cells were detected by MTT assay and clonogenic assay in vitro. Cell cycle was analyzed by flow cytometry. Morphological changes of the apoptosis of cancer cells were observed by Hochest 33258 staining. Western blot was performed to detect apoptosis-related gene expression. Results Ethyl acetate extract inhibited the growth of A549 cells but not in HFL-1 cells. Compared with controls, administration of 10 μg/mL ethyl acetate extract resulted in 73.86% decrease in colony formation (P < 0.01), apoptotic rates of 33.86% (P < 0.01), and morphological changes of apoptosis in A549 cells. The expression of anti-apoptotic protein EGFR and ERK were significantly down-regulated (P < 0.01). Conclusion Ethyl acetate extract might inhibit proliferation and induce apoptosis in A549 cells via downregulation of EGFR/ERK signal transduction pathway. Therefore, ethyl acetate extract should be further separated in order to identify the material fundamentals on anti-cancer effect. |
| first_indexed | 2024-04-12T17:27:42Z |
| format | Article |
| id | doaj.art-9515a15382714389ba3dd8ce72bbfa25 |
| institution | Directory Open Access Journal |
| issn | 1009-3419 1999-6187 |
| language | zho |
| last_indexed | 2024-04-12T17:27:42Z |
| publishDate | 2010-05-01 |
| publisher | Chinese Anti-Cancer Association; Chinese Antituberculosis Association |
| record_format | Article |
| series | Chinese Journal of Lung Cancer |
| spelling | doaj.art-9515a15382714389ba3dd8ce72bbfa252022-12-22T03:23:16ZzhoChinese Anti-Cancer Association; Chinese Antituberculosis AssociationChinese Journal of Lung Cancer1009-34191999-61872010-05-01135477482Mechanisms of Proliferative Inhibition by Maimendong & Qianjinweijing Decoction in A549 CellsXu ZHANGJin’ao DUANYuping TANGYuhui ZHOUZhen ZHANBackground and objective Traditional Chinese medicine is an approach for malignant tumor treatment with Chinese characteristics. The aim of this study is to investigate the inhibitory effects of Maimendong & qianjinweijing decoction extract on A549 human lung cancer cell line proliferation and explored its probable molecular mechanisms. Methods A549 cells were treated with drugs in different does and time. The effects on the proliferation of A549 cells were detected by MTT assay and clonogenic assay in vitro. Cell cycle was analyzed by flow cytometry. Morphological changes of the apoptosis of cancer cells were observed by Hochest 33258 staining. Western blot was performed to detect apoptosis-related gene expression. Results Ethyl acetate extract inhibited the growth of A549 cells but not in HFL-1 cells. Compared with controls, administration of 10 μg/mL ethyl acetate extract resulted in 73.86% decrease in colony formation (P < 0.01), apoptotic rates of 33.86% (P < 0.01), and morphological changes of apoptosis in A549 cells. The expression of anti-apoptotic protein EGFR and ERK were significantly down-regulated (P < 0.01). Conclusion Ethyl acetate extract might inhibit proliferation and induce apoptosis in A549 cells via downregulation of EGFR/ERK signal transduction pathway. Therefore, ethyl acetate extract should be further separated in order to identify the material fundamentals on anti-cancer effect.http://www.lungca.org/index.php?journal=01&page=article&op=viewFile&path[]=10.3779%2Fj.issn.1009-3419.2010.05.18&path[]=1484Lung neoplasmsProliferationApoptosisSignal transduction |
| spellingShingle | Xu ZHANG Jin’ao DUAN Yuping TANG Yuhui ZHOU Zhen ZHAN Mechanisms of Proliferative Inhibition by Maimendong & Qianjinweijing Decoction in A549 Cells Chinese Journal of Lung Cancer Lung neoplasms Proliferation Apoptosis Signal transduction |
| title | Mechanisms of Proliferative Inhibition by Maimendong & Qianjinweijing Decoction in A549 Cells |
| title_full | Mechanisms of Proliferative Inhibition by Maimendong & Qianjinweijing Decoction in A549 Cells |
| title_fullStr | Mechanisms of Proliferative Inhibition by Maimendong & Qianjinweijing Decoction in A549 Cells |
| title_full_unstemmed | Mechanisms of Proliferative Inhibition by Maimendong & Qianjinweijing Decoction in A549 Cells |
| title_short | Mechanisms of Proliferative Inhibition by Maimendong & Qianjinweijing Decoction in A549 Cells |
| title_sort | mechanisms of proliferative inhibition by maimendong qianjinweijing decoction in a549 cells |
| topic | Lung neoplasms Proliferation Apoptosis Signal transduction |
| url | http://www.lungca.org/index.php?journal=01&page=article&op=viewFile&path[]=10.3779%2Fj.issn.1009-3419.2010.05.18&path[]=1484 |
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