Identification of <it>Lactobacillus plantarum </it>genes modulating the cytokine response of human peripheral blood mononuclear cells
<p>Abstract</p> <p>Background</p> <p>Modulation of the immune system is one of the most plausible mechanisms underlying the beneficial effects of probiotic bacteria on human health. Presently, the specific probiotic cell products responsible for immunomodulation are lar...
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BMC
2010-11-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/10/293 |
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| author | Molenaar Douwe Meijerink Marjolein van Hemert Saskia Bron Peter A de Vos Paul Kleerebezem Michiel Wells Jerry M Marco Maria L |
| author_facet | Molenaar Douwe Meijerink Marjolein van Hemert Saskia Bron Peter A de Vos Paul Kleerebezem Michiel Wells Jerry M Marco Maria L |
| author_sort | Molenaar Douwe |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Modulation of the immune system is one of the most plausible mechanisms underlying the beneficial effects of probiotic bacteria on human health. Presently, the specific probiotic cell products responsible for immunomodulation are largely unknown. In this study, the genetic and phenotypic diversity of strains of the <it>Lactobacillus plantarum </it>species were investigated to identify genes of <it>L. plantarum </it>with the potential to influence the amounts of cytokines interleukin 10 (IL-10) and IL-12 and the ratio of IL-10/IL-12 produced by peripheral blood mononuclear cells (PBMCs).</p> <p>Results</p> <p>A total of 42 <it>Lactobacillus plantarum </it>strains isolated from diverse environmental and human sources were evaluated for their capacity to stimulate cytokine production in PBMCs. The <it>L. plantarum </it>strains induced the secretion of the anti-inflammatory cytokine IL-10 over an average 14-fold range and secretion of the pro-inflammatory cytokine IL-12 over an average 16-fold range. Comparisons of the strain-specific cytokine responses of PBMCs to comparative genome hybridization profiles obtained with <it>L. plantarum </it>WCFS1 DNA microarrays (also termed gene-trait matching) resulted in the identification of 6 candidate genetic loci with immunomodulatory capacities. These loci included genes encoding an <it>N</it>-acetyl-glucosamine/galactosamine phosphotransferase system, the LamBDCA quorum sensing system, and components of the plantaricin (bacteriocin) biosynthesis and transport pathway. Deletion of these genes in <it>L. plantarum </it>WCFS1 resulted in growth phase-dependent changes in the PBMC IL-10 and IL-12 cytokine profiles compared with wild-type cells.</p> <p>Conclusions</p> <p>The altered PBMC cytokine profiles obtained with the <it>L. plantarum </it>WCFS1 mutants were in good agreement with the predictions made by gene-trait matching for the 42 <it>L. plantarum </it>strains. This study therefore resulted in the identification of genes present in certain strains of <it>L. plantarum </it>which might be responsible for the stimulation of anti- or pro-inflammatory immune responses in the gut.</p> |
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| spelling | doaj.art-9515c024c0a04bfa94892e9352852a122022-12-21T20:39:33ZengBMCBMC Microbiology1471-21802010-11-0110129310.1186/1471-2180-10-293Identification of <it>Lactobacillus plantarum </it>genes modulating the cytokine response of human peripheral blood mononuclear cellsMolenaar DouweMeijerink Marjoleinvan Hemert SaskiaBron Peter Ade Vos PaulKleerebezem MichielWells Jerry MMarco Maria L<p>Abstract</p> <p>Background</p> <p>Modulation of the immune system is one of the most plausible mechanisms underlying the beneficial effects of probiotic bacteria on human health. Presently, the specific probiotic cell products responsible for immunomodulation are largely unknown. In this study, the genetic and phenotypic diversity of strains of the <it>Lactobacillus plantarum </it>species were investigated to identify genes of <it>L. plantarum </it>with the potential to influence the amounts of cytokines interleukin 10 (IL-10) and IL-12 and the ratio of IL-10/IL-12 produced by peripheral blood mononuclear cells (PBMCs).</p> <p>Results</p> <p>A total of 42 <it>Lactobacillus plantarum </it>strains isolated from diverse environmental and human sources were evaluated for their capacity to stimulate cytokine production in PBMCs. The <it>L. plantarum </it>strains induced the secretion of the anti-inflammatory cytokine IL-10 over an average 14-fold range and secretion of the pro-inflammatory cytokine IL-12 over an average 16-fold range. Comparisons of the strain-specific cytokine responses of PBMCs to comparative genome hybridization profiles obtained with <it>L. plantarum </it>WCFS1 DNA microarrays (also termed gene-trait matching) resulted in the identification of 6 candidate genetic loci with immunomodulatory capacities. These loci included genes encoding an <it>N</it>-acetyl-glucosamine/galactosamine phosphotransferase system, the LamBDCA quorum sensing system, and components of the plantaricin (bacteriocin) biosynthesis and transport pathway. Deletion of these genes in <it>L. plantarum </it>WCFS1 resulted in growth phase-dependent changes in the PBMC IL-10 and IL-12 cytokine profiles compared with wild-type cells.</p> <p>Conclusions</p> <p>The altered PBMC cytokine profiles obtained with the <it>L. plantarum </it>WCFS1 mutants were in good agreement with the predictions made by gene-trait matching for the 42 <it>L. plantarum </it>strains. This study therefore resulted in the identification of genes present in certain strains of <it>L. plantarum </it>which might be responsible for the stimulation of anti- or pro-inflammatory immune responses in the gut.</p>http://www.biomedcentral.com/1471-2180/10/293 |
| spellingShingle | Molenaar Douwe Meijerink Marjolein van Hemert Saskia Bron Peter A de Vos Paul Kleerebezem Michiel Wells Jerry M Marco Maria L Identification of <it>Lactobacillus plantarum </it>genes modulating the cytokine response of human peripheral blood mononuclear cells BMC Microbiology |
| title | Identification of <it>Lactobacillus plantarum </it>genes modulating the cytokine response of human peripheral blood mononuclear cells |
| title_full | Identification of <it>Lactobacillus plantarum </it>genes modulating the cytokine response of human peripheral blood mononuclear cells |
| title_fullStr | Identification of <it>Lactobacillus plantarum </it>genes modulating the cytokine response of human peripheral blood mononuclear cells |
| title_full_unstemmed | Identification of <it>Lactobacillus plantarum </it>genes modulating the cytokine response of human peripheral blood mononuclear cells |
| title_short | Identification of <it>Lactobacillus plantarum </it>genes modulating the cytokine response of human peripheral blood mononuclear cells |
| title_sort | identification of it lactobacillus plantarum it genes modulating the cytokine response of human peripheral blood mononuclear cells |
| url | http://www.biomedcentral.com/1471-2180/10/293 |
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