A Biflavonoid-Rich Extract from <i>Selaginella doederleinii</i> Hieron. against Throat Carcinoma via Akt/Bad and IKKβ/NF-κB/COX-2 Pathways
<i>Selaginella doederleinii</i> Hieron. is a common pharmacological plant, and this folk herbal medicine and its complex preparations have been widely used for the treatment of throat carcinoma (TC) and several associated complications in traditional Chinese medicine. This study was aime...
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MDPI AG
2022-12-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/15/12/1505 |
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| author | Sisi Wang Dingrong Wan Wenqi Liu Xinyi Kang Xiuteng Zhou Fatemeh Sefidkon Mohaddesehossadat Mahmoud Zadeh Hosseini Ting Zhang Xin Pan Xinzhou Yang |
| author_facet | Sisi Wang Dingrong Wan Wenqi Liu Xinyi Kang Xiuteng Zhou Fatemeh Sefidkon Mohaddesehossadat Mahmoud Zadeh Hosseini Ting Zhang Xin Pan Xinzhou Yang |
| author_sort | Sisi Wang |
| collection | DOAJ |
| description | <i>Selaginella doederleinii</i> Hieron. is a common pharmacological plant, and this folk herbal medicine and its complex preparations have been widely used for the treatment of throat carcinoma (TC) and several associated complications in traditional Chinese medicine. This study was aimed at investigating the specific anti-throat carcinoma impacts and potential mechanisms of a biflavonoid-rich extract from <i>S. doederleinii</i> (SD-BFRE). The phytochemical profiling of SD-BFRE was performed by HPLC-ESI-QTOF-MS and UPLC-PDA, and the detailed pharmacological effects and mechanisms were respectively evaluated in vitro and in vivo. MTT assay, the Transwell assay and flow cytometry were performed to evaluate the abilities of SD-BFRE on inhibiting cell infiltrative growth in TC cells (Hep-2 and FaDu) in in vitro experiments. In vivo experiments used Hep-2 tumor-bearing nude mice to evaluate the anti-TC effect of SD-BFRE. Western blotting was used to explore the potential apoptotic pathway of TC cells. Here, we found that SD-BFRE exhibited anti-proliferation and pro-apoptotic effects in TC cells. Mechanistic studies have identified that SD-BFRE can suppress the activity of IKKβ and IκB-α kinase and then down-regulate the effector proteins of NF-κB/COX-2 signaling. Moreover, SD-BFRE induced apoptosis partly by regulating the Akt/Bad/caspase signaling pathway. Taken together, this study firstly demonstrated that SD-BFRE exerted its anti-TC effects by way of IKKβ/NF-κB/COX-2 and Akt/Bad pathways and might represent a potential chemotherapeutic agent for throat carcinoma. |
| first_indexed | 2024-03-09T15:59:30Z |
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| id | doaj.art-951995de0a9d45b8bee4e9edeb20361e |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-09T15:59:30Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-951995de0a9d45b8bee4e9edeb20361e2023-11-24T17:16:20ZengMDPI AGPharmaceuticals1424-82472022-12-011512150510.3390/ph15121505A Biflavonoid-Rich Extract from <i>Selaginella doederleinii</i> Hieron. against Throat Carcinoma via Akt/Bad and IKKβ/NF-κB/COX-2 PathwaysSisi Wang0Dingrong Wan1Wenqi Liu2Xinyi Kang3Xiuteng Zhou4Fatemeh Sefidkon5Mohaddesehossadat Mahmoud Zadeh Hosseini6Ting Zhang7Xin Pan8Xinzhou Yang9School of Pharmaceutical Sciences, South-Central Minzu University, 182 Min-Zu Road, Wuhan 430074, ChinaSchool of Pharmaceutical Sciences, South-Central Minzu University, 182 Min-Zu Road, Wuhan 430074, ChinaSchool of Pharmaceutical Sciences, South-Central Minzu University, 182 Min-Zu Road, Wuhan 430074, ChinaSchool of Pharmaceutical Sciences, South-Central Minzu University, 182 Min-Zu Road, Wuhan 430074, ChinaState Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaResearch Division of Medicinal Plants, Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran P.O. Box 13185-116, IranCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaSchool of Pharmaceutical Sciences, South-Central Minzu University, 182 Min-Zu Road, Wuhan 430074, ChinaSchool of Pharmaceutical Sciences, South-Central Minzu University, 182 Min-Zu Road, Wuhan 430074, ChinaSchool of Pharmaceutical Sciences, South-Central Minzu University, 182 Min-Zu Road, Wuhan 430074, China<i>Selaginella doederleinii</i> Hieron. is a common pharmacological plant, and this folk herbal medicine and its complex preparations have been widely used for the treatment of throat carcinoma (TC) and several associated complications in traditional Chinese medicine. This study was aimed at investigating the specific anti-throat carcinoma impacts and potential mechanisms of a biflavonoid-rich extract from <i>S. doederleinii</i> (SD-BFRE). The phytochemical profiling of SD-BFRE was performed by HPLC-ESI-QTOF-MS and UPLC-PDA, and the detailed pharmacological effects and mechanisms were respectively evaluated in vitro and in vivo. MTT assay, the Transwell assay and flow cytometry were performed to evaluate the abilities of SD-BFRE on inhibiting cell infiltrative growth in TC cells (Hep-2 and FaDu) in in vitro experiments. In vivo experiments used Hep-2 tumor-bearing nude mice to evaluate the anti-TC effect of SD-BFRE. Western blotting was used to explore the potential apoptotic pathway of TC cells. Here, we found that SD-BFRE exhibited anti-proliferation and pro-apoptotic effects in TC cells. Mechanistic studies have identified that SD-BFRE can suppress the activity of IKKβ and IκB-α kinase and then down-regulate the effector proteins of NF-κB/COX-2 signaling. Moreover, SD-BFRE induced apoptosis partly by regulating the Akt/Bad/caspase signaling pathway. Taken together, this study firstly demonstrated that SD-BFRE exerted its anti-TC effects by way of IKKβ/NF-κB/COX-2 and Akt/Bad pathways and might represent a potential chemotherapeutic agent for throat carcinoma.https://www.mdpi.com/1424-8247/15/12/1505<i>Selaginella doederleinii</i>biflavonoid-rich extractionthroat cancerapoptosisNF-κBAkt |
| spellingShingle | Sisi Wang Dingrong Wan Wenqi Liu Xinyi Kang Xiuteng Zhou Fatemeh Sefidkon Mohaddesehossadat Mahmoud Zadeh Hosseini Ting Zhang Xin Pan Xinzhou Yang A Biflavonoid-Rich Extract from <i>Selaginella doederleinii</i> Hieron. against Throat Carcinoma via Akt/Bad and IKKβ/NF-κB/COX-2 Pathways Pharmaceuticals <i>Selaginella doederleinii</i> biflavonoid-rich extraction throat cancer apoptosis NF-κB Akt |
| title | A Biflavonoid-Rich Extract from <i>Selaginella doederleinii</i> Hieron. against Throat Carcinoma via Akt/Bad and IKKβ/NF-κB/COX-2 Pathways |
| title_full | A Biflavonoid-Rich Extract from <i>Selaginella doederleinii</i> Hieron. against Throat Carcinoma via Akt/Bad and IKKβ/NF-κB/COX-2 Pathways |
| title_fullStr | A Biflavonoid-Rich Extract from <i>Selaginella doederleinii</i> Hieron. against Throat Carcinoma via Akt/Bad and IKKβ/NF-κB/COX-2 Pathways |
| title_full_unstemmed | A Biflavonoid-Rich Extract from <i>Selaginella doederleinii</i> Hieron. against Throat Carcinoma via Akt/Bad and IKKβ/NF-κB/COX-2 Pathways |
| title_short | A Biflavonoid-Rich Extract from <i>Selaginella doederleinii</i> Hieron. against Throat Carcinoma via Akt/Bad and IKKβ/NF-κB/COX-2 Pathways |
| title_sort | biflavonoid rich extract from i selaginella doederleinii i hieron against throat carcinoma via akt bad and ikkβ nf κb cox 2 pathways |
| topic | <i>Selaginella doederleinii</i> biflavonoid-rich extraction throat cancer apoptosis NF-κB Akt |
| url | https://www.mdpi.com/1424-8247/15/12/1505 |
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