DNA barcoding elucidates the new altitude record and range-extension of lesser-known bullfrog (Hoplobatrachus litoralis) in northeast India
The molecular information of lesser-known bullfrog, Hoplobatrachus litoralis is restricted from Bangladesh (type locality) and Myanmar. The morphological observation further evidenced the range-extension of this species from India and Myanmar. Here, we collected the H. litoralis specimen from the Da...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-07-01
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| Series: | Mitochondrial DNA. Part B. Resources |
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| Online Access: | http://dx.doi.org/10.1080/23802359.2020.1787259 |
| _version_ | 1797639162789101568 |
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| author | Shantanu Kundu Hmar Tlawmte Lalremsanga Jayaditya Purkayastha Lal Biakzuala Kailash Chandra Vikas Kumar |
| author_facet | Shantanu Kundu Hmar Tlawmte Lalremsanga Jayaditya Purkayastha Lal Biakzuala Kailash Chandra Vikas Kumar |
| author_sort | Shantanu Kundu |
| collection | DOAJ |
| description | The molecular information of lesser-known bullfrog, Hoplobatrachus litoralis is restricted from Bangladesh (type locality) and Myanmar. The morphological observation further evidenced the range-extension of this species from India and Myanmar. Here, we collected the H. litoralis specimen from the Dampa Tiger Reserve, Mizoram state and provides a new altitude record (268 m) and range-extension in northeast India. The DNA barcoding with mitochondrial Cytochrome b gene discriminates H. litoralis from other congeners with sufficient genetic distance (13.9% to 27.8%). The Bayesian phylogeny revealed monophyletic clustering of all five Hoplobatrachus species and showed the sister relationship of H. litoralis and H. tigerinus. The generated sequences of H. litoralis from northeast India depicted shallow divergence (1.5%) from the sequences generated from Bangladesh, indicates the possible genetic variability due to the alteration of the ecological niche. We suggested further physiological and molecular study with extensive sampling of this species to confirm their actual range distribution, high-altitude adaptation, and gene flow. |
| first_indexed | 2024-03-11T13:13:53Z |
| format | Article |
| id | doaj.art-951a4b1f75d746b5b38df0d8304d3cdc |
| institution | Directory Open Access Journal |
| issn | 2380-2359 |
| language | English |
| last_indexed | 2024-03-11T13:13:53Z |
| publishDate | 2020-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mitochondrial DNA. Part B. Resources |
| spelling | doaj.art-951a4b1f75d746b5b38df0d8304d3cdc2023-11-03T14:00:30ZengTaylor & Francis GroupMitochondrial DNA. Part B. Resources2380-23592020-07-01532668267210.1080/23802359.2020.17872591787259DNA barcoding elucidates the new altitude record and range-extension of lesser-known bullfrog (Hoplobatrachus litoralis) in northeast IndiaShantanu Kundu0Hmar Tlawmte Lalremsanga1Jayaditya Purkayastha2Lal Biakzuala3Kailash Chandra4Vikas Kumar5Centre for DNA Taxonomy, Molecular Systematics Division, Zoological Survey of IndiaDevelopmental Biology and Herpetology Laboratory, Department of Zoology, Mizoram UniversityHelp EarthDevelopmental Biology and Herpetology Laboratory, Department of Zoology, Mizoram UniversityCentre for DNA Taxonomy, Molecular Systematics Division, Zoological Survey of IndiaCentre for DNA Taxonomy, Molecular Systematics Division, Zoological Survey of IndiaThe molecular information of lesser-known bullfrog, Hoplobatrachus litoralis is restricted from Bangladesh (type locality) and Myanmar. The morphological observation further evidenced the range-extension of this species from India and Myanmar. Here, we collected the H. litoralis specimen from the Dampa Tiger Reserve, Mizoram state and provides a new altitude record (268 m) and range-extension in northeast India. The DNA barcoding with mitochondrial Cytochrome b gene discriminates H. litoralis from other congeners with sufficient genetic distance (13.9% to 27.8%). The Bayesian phylogeny revealed monophyletic clustering of all five Hoplobatrachus species and showed the sister relationship of H. litoralis and H. tigerinus. The generated sequences of H. litoralis from northeast India depicted shallow divergence (1.5%) from the sequences generated from Bangladesh, indicates the possible genetic variability due to the alteration of the ecological niche. We suggested further physiological and molecular study with extensive sampling of this species to confirm their actual range distribution, high-altitude adaptation, and gene flow.http://dx.doi.org/10.1080/23802359.2020.1787259indo-burmabullfrogmitochondrial dnaphylogenyrange extension |
| spellingShingle | Shantanu Kundu Hmar Tlawmte Lalremsanga Jayaditya Purkayastha Lal Biakzuala Kailash Chandra Vikas Kumar DNA barcoding elucidates the new altitude record and range-extension of lesser-known bullfrog (Hoplobatrachus litoralis) in northeast India Mitochondrial DNA. Part B. Resources indo-burma bullfrog mitochondrial dna phylogeny range extension |
| title | DNA barcoding elucidates the new altitude record and range-extension of lesser-known bullfrog (Hoplobatrachus litoralis) in northeast India |
| title_full | DNA barcoding elucidates the new altitude record and range-extension of lesser-known bullfrog (Hoplobatrachus litoralis) in northeast India |
| title_fullStr | DNA barcoding elucidates the new altitude record and range-extension of lesser-known bullfrog (Hoplobatrachus litoralis) in northeast India |
| title_full_unstemmed | DNA barcoding elucidates the new altitude record and range-extension of lesser-known bullfrog (Hoplobatrachus litoralis) in northeast India |
| title_short | DNA barcoding elucidates the new altitude record and range-extension of lesser-known bullfrog (Hoplobatrachus litoralis) in northeast India |
| title_sort | dna barcoding elucidates the new altitude record and range extension of lesser known bullfrog hoplobatrachus litoralis in northeast india |
| topic | indo-burma bullfrog mitochondrial dna phylogeny range extension |
| url | http://dx.doi.org/10.1080/23802359.2020.1787259 |
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