Analysis of Differential Metabolites between NFC and FC Bayberry Juice Based on UPLC-QTOF-MS
This study analyzed the differential composition between from concentrate (FC) and not from concentrate (NFC) bayberry juices by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). NFC and FC bayberry juice were distinguished from each other by or...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | zho |
Published: |
The editorial department of Science and Technology of Food Industry
2023-08-01
|
Series: | Shipin gongye ke-ji |
Subjects: | |
Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022080132 |
_version_ | 1797737859946381312 |
---|---|
author | Ruiyang SUN Xiaoting XUAN Yan CUI Xudong LIN Xingfeng SHAO Wenyi DENG Jiangang LING |
author_facet | Ruiyang SUN Xiaoting XUAN Yan CUI Xudong LIN Xingfeng SHAO Wenyi DENG Jiangang LING |
author_sort | Ruiyang SUN |
collection | DOAJ |
description | This study analyzed the differential composition between from concentrate (FC) and not from concentrate (NFC) bayberry juices by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). NFC and FC bayberry juice were distinguished from each other by orthogonal partial least squares discriminant analysis, and 9 compounds that significantly differed in abundance between them were selected and identified according to the variable importance in the projection and the fold-change. By comparison, the content of antioxidant active components such as 4-sulfobenzoate, 5-methyltetrahydrofolic acid, cyanidin, daphnoretin, xanthotoxol and isoxanthopterin in FC bayberry juice were relatively low, whereas the bitter substances (neohesperidin, hesperidin and quercetin-3-O-neohesperidin) were relatively high. Metabolic pathways were analyzed on account of differential metabolites and five metabolic pathways with great differences were identified, among which phenylalanine metabolism, biosynthesis of flavonoids and flavonols were the key metabolic pathways of different metabolites in NFC and FC bayberry juice. This study revealed the difference of metabolites between NFC and FC bayberry juice, which provided theoretical reference for the processing and identification of NFC bayberry juice. |
first_indexed | 2024-03-12T13:35:37Z |
format | Article |
id | doaj.art-951ba23d421441ada94e070c8f33fdaa |
institution | Directory Open Access Journal |
issn | 1002-0306 |
language | zho |
last_indexed | 2024-03-12T13:35:37Z |
publishDate | 2023-08-01 |
publisher | The editorial department of Science and Technology of Food Industry |
record_format | Article |
series | Shipin gongye ke-ji |
spelling | doaj.art-951ba23d421441ada94e070c8f33fdaa2023-08-24T06:00:17ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-08-01441527528210.13386/j.issn1002-0306.20220801322022080132-15Analysis of Differential Metabolites between NFC and FC Bayberry Juice Based on UPLC-QTOF-MSRuiyang SUN0Xiaoting XUAN1Yan CUI2Xudong LIN3Xingfeng SHAO4Wenyi DENG5Jiangang LING6College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315000, ChinaInstitute of Agricultural Products Processing, Ningbo Academy of Agricultural Sciences, Ningbo 315000, ChinaInstitute of Agricultural Products Processing, Ningbo Academy of Agricultural Sciences, Ningbo 315000, ChinaInstitute of Agricultural Products Processing, Ningbo Academy of Agricultural Sciences, Ningbo 315000, ChinaCollege of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315000, ChinaHunan Yipindongfang Biotechnology Co., Ltd., Changsha 410000, ChinaInstitute of Agricultural Products Processing, Ningbo Academy of Agricultural Sciences, Ningbo 315000, ChinaThis study analyzed the differential composition between from concentrate (FC) and not from concentrate (NFC) bayberry juices by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). NFC and FC bayberry juice were distinguished from each other by orthogonal partial least squares discriminant analysis, and 9 compounds that significantly differed in abundance between them were selected and identified according to the variable importance in the projection and the fold-change. By comparison, the content of antioxidant active components such as 4-sulfobenzoate, 5-methyltetrahydrofolic acid, cyanidin, daphnoretin, xanthotoxol and isoxanthopterin in FC bayberry juice were relatively low, whereas the bitter substances (neohesperidin, hesperidin and quercetin-3-O-neohesperidin) were relatively high. Metabolic pathways were analyzed on account of differential metabolites and five metabolic pathways with great differences were identified, among which phenylalanine metabolism, biosynthesis of flavonoids and flavonols were the key metabolic pathways of different metabolites in NFC and FC bayberry juice. This study revealed the difference of metabolites between NFC and FC bayberry juice, which provided theoretical reference for the processing and identification of NFC bayberry juice.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022080132not from concentrate bayberry juiceultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometrymetabolomicsmetabolic pathways |
spellingShingle | Ruiyang SUN Xiaoting XUAN Yan CUI Xudong LIN Xingfeng SHAO Wenyi DENG Jiangang LING Analysis of Differential Metabolites between NFC and FC Bayberry Juice Based on UPLC-QTOF-MS Shipin gongye ke-ji not from concentrate bayberry juice ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry metabolomics metabolic pathways |
title | Analysis of Differential Metabolites between NFC and FC Bayberry Juice Based on UPLC-QTOF-MS |
title_full | Analysis of Differential Metabolites between NFC and FC Bayberry Juice Based on UPLC-QTOF-MS |
title_fullStr | Analysis of Differential Metabolites between NFC and FC Bayberry Juice Based on UPLC-QTOF-MS |
title_full_unstemmed | Analysis of Differential Metabolites between NFC and FC Bayberry Juice Based on UPLC-QTOF-MS |
title_short | Analysis of Differential Metabolites between NFC and FC Bayberry Juice Based on UPLC-QTOF-MS |
title_sort | analysis of differential metabolites between nfc and fc bayberry juice based on uplc qtof ms |
topic | not from concentrate bayberry juice ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry metabolomics metabolic pathways |
url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022080132 |
work_keys_str_mv | AT ruiyangsun analysisofdifferentialmetabolitesbetweennfcandfcbayberryjuicebasedonuplcqtofms AT xiaotingxuan analysisofdifferentialmetabolitesbetweennfcandfcbayberryjuicebasedonuplcqtofms AT yancui analysisofdifferentialmetabolitesbetweennfcandfcbayberryjuicebasedonuplcqtofms AT xudonglin analysisofdifferentialmetabolitesbetweennfcandfcbayberryjuicebasedonuplcqtofms AT xingfengshao analysisofdifferentialmetabolitesbetweennfcandfcbayberryjuicebasedonuplcqtofms AT wenyideng analysisofdifferentialmetabolitesbetweennfcandfcbayberryjuicebasedonuplcqtofms AT jiangangling analysisofdifferentialmetabolitesbetweennfcandfcbayberryjuicebasedonuplcqtofms |