Poultry infected with Mycoplasma gallisepticum or Mycoplasma synoviae produce antibodies to their cysteine protease CysP
Major poultry pathogens, Mycoplasma gallisepticum and Mycoplasma synoviae possess cysteine protease CysP, which in vitro cleaves chicken immunoglobulin G (IgG) into Fab (antigen-binding fragment) and Fc (crystallisable region fragment). We used recombinant CysP of M. synoviae (rCysP) in enzyme immun...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2013-12-01
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| Series: | Acta Agriculturae Slovenica |
| Subjects: | |
| Online Access: | https://journals.uni-lj.si/aas/article/view/14903 |
| _version_ | 1797393209380306944 |
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| author | Ivanka CIZELJ Rebeka Lucijana BERČIČ Daliborka DUŠANIĆ Mateja BENČINA Mojca NARAT Olga ZORMAN-ROJS Dušan BENČINA |
| author_facet | Ivanka CIZELJ Rebeka Lucijana BERČIČ Daliborka DUŠANIĆ Mateja BENČINA Mojca NARAT Olga ZORMAN-ROJS Dušan BENČINA |
| author_sort | Ivanka CIZELJ |
| collection | DOAJ |
| description | Major poultry pathogens, Mycoplasma gallisepticum and Mycoplasma synoviae possess cysteine protease CysP, which in vitro cleaves chicken immunoglobulin G (IgG) into Fab (antigen-binding fragment) and Fc (crystallisable region fragment). We used recombinant CysP of M. synoviae (rCysP) in enzyme immunoassays to detect antibodies to CysP in sera, synovial fluids, and in washings of respiratory tract and oviducts of chickens and turkeys experimentally or naturally infected with M. gallisepticum or M. synoviae. In poultry infected with M. synoviae, 70.4 % of samples contained antibodies reacting with rCysP. In birds infected with M. gallisepticum we detected CysP antibodies in 63.1 % of samples. Our data demonstrate that CysP is immunogenic for chickens and turkeys and indicate that M. gallisepticum and M. synoviae infecting chickens and turkeys synthesise CysP in vivo. This is the first study demonstrating that proteases of any Mycoplasma species can induce production of specific antibodies in the natural host.
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| first_indexed | 2024-03-08T23:58:56Z |
| format | Article |
| id | doaj.art-951c7dea986e4e89baca78b628423455 |
| institution | Directory Open Access Journal |
| issn | 1854-1941 |
| language | English |
| last_indexed | 2024-03-08T23:58:56Z |
| publishDate | 2013-12-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj.art-951c7dea986e4e89baca78b6284234552023-12-12T22:13:47ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412013-12-011021Poultry infected with Mycoplasma gallisepticum or Mycoplasma synoviae produce antibodies to their cysteine protease CysPIvanka CIZELJ0Rebeka Lucijana BERČIČ1Daliborka DUŠANIĆ2Mateja BENČINA3Mojca NARAT4Olga ZORMAN-ROJS5Dušan BENČINA6Univ. of Ljubljana, Biotechnical Fac., Dept. of Animal Science., Groblje 3, SI-1230 DomžaleUniv. of Ljubljana, Biotechnical Fac., Dept. of Animal Science., Groblje 3, SI-1230 DomžaleUniv. of Ljubljana, Biotechnical Fac., Dept. of Animal Science., Groblje 3, SI-1230 DomžaleUniv. of Ljubljana, Biotechnical Fac., Dept. of Animal Science., Groblje 3, SI-1230 Domžale; Univ. of Ljubljana, Fac. of Medicine, Vrazov trg 2, SI-1104 Ljubljana, SloveniaUniv. of Ljubljana, Biotechnical Fac., Dept. of Animal Science., Groblje 3, SI-1230 DomžaleUniv. of Ljubljana, Veterinary Fac., Gerbičeva 60, SI-1000 Ljubljana, SloveniaUniv. of Ljubljana, Biotechnical Fac., Dept. of Animal Science., Groblje 3, SI-1230 DomžaleMajor poultry pathogens, Mycoplasma gallisepticum and Mycoplasma synoviae possess cysteine protease CysP, which in vitro cleaves chicken immunoglobulin G (IgG) into Fab (antigen-binding fragment) and Fc (crystallisable region fragment). We used recombinant CysP of M. synoviae (rCysP) in enzyme immunoassays to detect antibodies to CysP in sera, synovial fluids, and in washings of respiratory tract and oviducts of chickens and turkeys experimentally or naturally infected with M. gallisepticum or M. synoviae. In poultry infected with M. synoviae, 70.4 % of samples contained antibodies reacting with rCysP. In birds infected with M. gallisepticum we detected CysP antibodies in 63.1 % of samples. Our data demonstrate that CysP is immunogenic for chickens and turkeys and indicate that M. gallisepticum and M. synoviae infecting chickens and turkeys synthesise CysP in vivo. This is the first study demonstrating that proteases of any Mycoplasma species can induce production of specific antibodies in the natural host. https://journals.uni-lj.si/aas/article/view/14903poultryinfectionsimmunologyimmunogenicitycysteine proteaseMycoplasma synoviae |
| spellingShingle | Ivanka CIZELJ Rebeka Lucijana BERČIČ Daliborka DUŠANIĆ Mateja BENČINA Mojca NARAT Olga ZORMAN-ROJS Dušan BENČINA Poultry infected with Mycoplasma gallisepticum or Mycoplasma synoviae produce antibodies to their cysteine protease CysP Acta Agriculturae Slovenica poultry infections immunology immunogenicity cysteine protease Mycoplasma synoviae |
| title | Poultry infected with Mycoplasma gallisepticum or Mycoplasma synoviae produce antibodies to their cysteine protease CysP |
| title_full | Poultry infected with Mycoplasma gallisepticum or Mycoplasma synoviae produce antibodies to their cysteine protease CysP |
| title_fullStr | Poultry infected with Mycoplasma gallisepticum or Mycoplasma synoviae produce antibodies to their cysteine protease CysP |
| title_full_unstemmed | Poultry infected with Mycoplasma gallisepticum or Mycoplasma synoviae produce antibodies to their cysteine protease CysP |
| title_short | Poultry infected with Mycoplasma gallisepticum or Mycoplasma synoviae produce antibodies to their cysteine protease CysP |
| title_sort | poultry infected with mycoplasma gallisepticum or mycoplasma synoviae produce antibodies to their cysteine protease cysp |
| topic | poultry infections immunology immunogenicity cysteine protease Mycoplasma synoviae |
| url | https://journals.uni-lj.si/aas/article/view/14903 |
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