LncRNA NORAD facilitates oral squamous cell carcinoma progression by sponging miR-577 to enhance TPM4
Abstract Background Long non-coding RNAs (lncRNAs) have been reported to be vital factors to affect the expression of genes and proteins. Also, it has been proved that the abnormal expression or mutation of lncRNAs stands as a signal of metastasis and proliferation of cancer. Nevertheless, the major...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-01-01
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| Series: | Biology Direct |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13062-021-00299-2 |
| _version_ | 1818940575094669312 |
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| author | Change Qi Jianwei Liu Pengnv Guo Yali Xu Jing Hu Xiaomei Han |
| author_facet | Change Qi Jianwei Liu Pengnv Guo Yali Xu Jing Hu Xiaomei Han |
| author_sort | Change Qi |
| collection | DOAJ |
| description | Abstract Background Long non-coding RNAs (lncRNAs) have been reported to be vital factors to affect the expression of genes and proteins. Also, it has been proved that the abnormal expression or mutation of lncRNAs stands as a signal of metastasis and proliferation of cancer. Nevertheless, the majority of lncRNAs still need to be explored in abundant cancers especially in oral squamous cell carcinoma (OSCC). Methods RT-qPCR assays were applied to test the expression of RNAs. Mechanism assays were performed to verify the combination among NORAD, TPM4 and miR-577. Also, functional assays were conducted to verify the function of RNAs on OSCC cells. Results LncRNA NORAD was highly expressed in OSCC tissues and cells. NORAD silencing repressed the biological behaviors of OSCC cells. MiR-577 was found in OSCC with low expression, and RIP assays illustrated that NORAD, miR-577 and TPM4 coexisted in RNA-induced silencing complexes. Rescue assays proved that the overexpression of TPM4 could recover the effect of NORAD silencing on OSCC progression. Conclusions It was revealed that NORAD functioned as a tumor promoter to sponge miR-577 thus elevating TPM4 in OSCC, which indicated that NORAD was worthy to be studied as a target for the treatment of OSCC. |
| first_indexed | 2024-12-20T06:41:49Z |
| format | Article |
| id | doaj.art-951db47433b549c0a0ecf923f1b8ca85 |
| institution | Directory Open Access Journal |
| issn | 1745-6150 |
| language | English |
| last_indexed | 2024-12-20T06:41:49Z |
| publishDate | 2022-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biology Direct |
| spelling | doaj.art-951db47433b549c0a0ecf923f1b8ca852022-12-21T19:49:49ZengBMCBiology Direct1745-61502022-01-0117111010.1186/s13062-021-00299-2LncRNA NORAD facilitates oral squamous cell carcinoma progression by sponging miR-577 to enhance TPM4Change Qi0Jianwei Liu1Pengnv Guo2Yali Xu3Jing Hu4Xiaomei Han5Department of Oral Implantology, Affiliated Hospital of Chifeng UniversityDepartment of Oral and Maxillofacial Surgery, Affiliated Hospital of Chifeng UniversityDepartment of Oral Implantology, Affiliated Hospital of Chifeng UniversityDepartment of Oral Implantology, Affiliated Hospital of Chifeng UniversityDepartment of Oral Implantology, Affiliated Hospital of Chifeng UniversityDepartment of Oral Implantology, Affiliated Hospital of Chifeng UniversityAbstract Background Long non-coding RNAs (lncRNAs) have been reported to be vital factors to affect the expression of genes and proteins. Also, it has been proved that the abnormal expression or mutation of lncRNAs stands as a signal of metastasis and proliferation of cancer. Nevertheless, the majority of lncRNAs still need to be explored in abundant cancers especially in oral squamous cell carcinoma (OSCC). Methods RT-qPCR assays were applied to test the expression of RNAs. Mechanism assays were performed to verify the combination among NORAD, TPM4 and miR-577. Also, functional assays were conducted to verify the function of RNAs on OSCC cells. Results LncRNA NORAD was highly expressed in OSCC tissues and cells. NORAD silencing repressed the biological behaviors of OSCC cells. MiR-577 was found in OSCC with low expression, and RIP assays illustrated that NORAD, miR-577 and TPM4 coexisted in RNA-induced silencing complexes. Rescue assays proved that the overexpression of TPM4 could recover the effect of NORAD silencing on OSCC progression. Conclusions It was revealed that NORAD functioned as a tumor promoter to sponge miR-577 thus elevating TPM4 in OSCC, which indicated that NORAD was worthy to be studied as a target for the treatment of OSCC.https://doi.org/10.1186/s13062-021-00299-2NORADmiR-577TPM4Oral squamous cell carcinoma |
| spellingShingle | Change Qi Jianwei Liu Pengnv Guo Yali Xu Jing Hu Xiaomei Han LncRNA NORAD facilitates oral squamous cell carcinoma progression by sponging miR-577 to enhance TPM4 Biology Direct NORAD miR-577 TPM4 Oral squamous cell carcinoma |
| title | LncRNA NORAD facilitates oral squamous cell carcinoma progression by sponging miR-577 to enhance TPM4 |
| title_full | LncRNA NORAD facilitates oral squamous cell carcinoma progression by sponging miR-577 to enhance TPM4 |
| title_fullStr | LncRNA NORAD facilitates oral squamous cell carcinoma progression by sponging miR-577 to enhance TPM4 |
| title_full_unstemmed | LncRNA NORAD facilitates oral squamous cell carcinoma progression by sponging miR-577 to enhance TPM4 |
| title_short | LncRNA NORAD facilitates oral squamous cell carcinoma progression by sponging miR-577 to enhance TPM4 |
| title_sort | lncrna norad facilitates oral squamous cell carcinoma progression by sponging mir 577 to enhance tpm4 |
| topic | NORAD miR-577 TPM4 Oral squamous cell carcinoma |
| url | https://doi.org/10.1186/s13062-021-00299-2 |
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