Comparison of total antibody and interferon-γ T-cell responses in patients following infection with brucellosis in Georgia
Summary: Brucellosis is an ancient disease that still remains a significant threat to humans and is typically linked to exposure to infected animals and/or consumption of unpasteurized animal products. Despite this history, we have a relatively limited understanding of the host characteristics of th...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2012-10-01
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| Series: | Journal of Infection and Public Health |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1876034112000846 |
| _version_ | 1819043640471715840 |
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| author | T. Akhvlediani N. Chitadze T.R. Laws M. Makharadze M. Chubinidze Sh. Tsanava N.J. Commander S.D. Perkins E.H. Dyson R.G. Rivard M.J. Hepburn A.J.H. Simpson P. Imnadze N. Trapaidze |
| author_facet | T. Akhvlediani N. Chitadze T.R. Laws M. Makharadze M. Chubinidze Sh. Tsanava N.J. Commander S.D. Perkins E.H. Dyson R.G. Rivard M.J. Hepburn A.J.H. Simpson P. Imnadze N. Trapaidze |
| author_sort | T. Akhvlediani |
| collection | DOAJ |
| description | Summary: Brucellosis is an ancient disease that still remains a significant threat to humans and is typically linked to exposure to infected animals and/or consumption of unpasteurized animal products. Despite this history, we have a relatively limited understanding of the host characteristics of this disease; consequently, further research is necessary. In this study, we examined the humoral immune response in 43 Georgian individuals that had been diagnosed with brucellosis 3–12 months before enrollment in the study, many of whom still had symptoms after the completion of antibiotic therapy. In total, 35 of 43 (83%) of the patients had antibodies that bound to Brucella lipopolysaccharide (LPS) by COMPELISA, and 34 of 38 (89%) patients had demonstrable specific antibodies to Brucellergene™ antigens; the results from the two ELISAs were highly correlated (p = 0.031, r = 0.851). We also studied the cellular immune responses in 15 patients. All of the patients generated interferon (IFN)-γ in response to ex vivo stimulation with Brucella protein antigens, and the majority of the patients maintained measurable humoral responses to both LPS and protein antigens. From this initial study, we conclude that measurement of antibody and of cellular (IFN-γ) responses to brucellergene OCB protein epitopes may be worthy of further investigation as an alternative or adjunct to current diagnostics. Keywords: Brucella, Immune response, ELISPOT, ELISA, Brucellin, LPS |
| first_indexed | 2024-12-21T10:00:00Z |
| format | Article |
| id | doaj.art-951dc61e0dda4f39a5f460cff8e427a1 |
| institution | Directory Open Access Journal |
| issn | 1876-0341 |
| language | English |
| last_indexed | 2024-12-21T10:00:00Z |
| publishDate | 2012-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Infection and Public Health |
| spelling | doaj.art-951dc61e0dda4f39a5f460cff8e427a12022-12-21T19:07:57ZengElsevierJournal of Infection and Public Health1876-03412012-10-0155321331Comparison of total antibody and interferon-γ T-cell responses in patients following infection with brucellosis in GeorgiaT. Akhvlediani0N. Chitadze1T.R. Laws2M. Makharadze3M. Chubinidze4Sh. Tsanava5N.J. Commander6S.D. Perkins7E.H. Dyson8R.G. Rivard9M.J. Hepburn10A.J.H. Simpson11P. Imnadze12N. Trapaidze13Clinical Research Unit (CRU), Technology Management Company (TMC), Tbilisi, GeorgiaNational Center for Disease Control and Public Health (NCDC), Tbilisi, GeorgiaDefence Science and Technology Laboratory (DSTL), Porton Down, Salisbury, Wiltshire SP4 0JQ, UKS. Virsaladze Scientific Research Institute of Medical Parasitology and Tropical Medicine, Tbilisi, GeorgiaNational Center for Disease Control and Public Health (NCDC), Tbilisi, GeorgiaNational Center for Disease Control and Public Health (NCDC), Tbilisi, GeorgiaDefence Science and Technology Laboratory (DSTL), Porton Down, Salisbury, Wiltshire SP4 0JQ, UK; Veterinary Laboratories Agency (VLA), Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UKDefence Science and Technology Laboratory (DSTL), Porton Down, Salisbury, Wiltshire SP4 0JQ, UKDefence Science and Technology Laboratory (DSTL), Porton Down, Salisbury, Wiltshire SP4 0JQ, UKU.S. Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, MD, USAU.S. Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, MD, USADefence Science and Technology Laboratory (DSTL), Porton Down, Salisbury, Wiltshire SP4 0JQ, UKNational Center for Disease Control and Public Health (NCDC), Tbilisi, GeorgiaClinical Research Unit (CRU), Technology Management Company (TMC), Tbilisi, Georgia; National Center for Disease Control and Public Health (NCDC), Tbilisi, Georgia; Corresponding author at: National Center for Disease Control and Public Health (NCDC), 9 Asatiani st., Tbilisi 0177, Georgia. Tel.: +995 99 54 3004; fax: +995 32 31 1485.Summary: Brucellosis is an ancient disease that still remains a significant threat to humans and is typically linked to exposure to infected animals and/or consumption of unpasteurized animal products. Despite this history, we have a relatively limited understanding of the host characteristics of this disease; consequently, further research is necessary. In this study, we examined the humoral immune response in 43 Georgian individuals that had been diagnosed with brucellosis 3–12 months before enrollment in the study, many of whom still had symptoms after the completion of antibiotic therapy. In total, 35 of 43 (83%) of the patients had antibodies that bound to Brucella lipopolysaccharide (LPS) by COMPELISA, and 34 of 38 (89%) patients had demonstrable specific antibodies to Brucellergene™ antigens; the results from the two ELISAs were highly correlated (p = 0.031, r = 0.851). We also studied the cellular immune responses in 15 patients. All of the patients generated interferon (IFN)-γ in response to ex vivo stimulation with Brucella protein antigens, and the majority of the patients maintained measurable humoral responses to both LPS and protein antigens. From this initial study, we conclude that measurement of antibody and of cellular (IFN-γ) responses to brucellergene OCB protein epitopes may be worthy of further investigation as an alternative or adjunct to current diagnostics. Keywords: Brucella, Immune response, ELISPOT, ELISA, Brucellin, LPShttp://www.sciencedirect.com/science/article/pii/S1876034112000846 |
| spellingShingle | T. Akhvlediani N. Chitadze T.R. Laws M. Makharadze M. Chubinidze Sh. Tsanava N.J. Commander S.D. Perkins E.H. Dyson R.G. Rivard M.J. Hepburn A.J.H. Simpson P. Imnadze N. Trapaidze Comparison of total antibody and interferon-γ T-cell responses in patients following infection with brucellosis in Georgia Journal of Infection and Public Health |
| title | Comparison of total antibody and interferon-γ T-cell responses in patients following infection with brucellosis in Georgia |
| title_full | Comparison of total antibody and interferon-γ T-cell responses in patients following infection with brucellosis in Georgia |
| title_fullStr | Comparison of total antibody and interferon-γ T-cell responses in patients following infection with brucellosis in Georgia |
| title_full_unstemmed | Comparison of total antibody and interferon-γ T-cell responses in patients following infection with brucellosis in Georgia |
| title_short | Comparison of total antibody and interferon-γ T-cell responses in patients following infection with brucellosis in Georgia |
| title_sort | comparison of total antibody and interferon γ t cell responses in patients following infection with brucellosis in georgia |
| url | http://www.sciencedirect.com/science/article/pii/S1876034112000846 |
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