Digital Marine Bioprospecting: Mining New Neurotoxin Drug Candidates from the Transcriptomes of Cold-Water Sea Anemones
Marine bioprospecting is the search for new marine bioactive compounds and large-scale screening in extracts represents the traditional approach. Here, we report an alternative complementary protocol, called digital marine bioprospecting, based on deep sequencing of transcriptomes. We sequenced the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2012-10-01
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| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1660-3397/10/10/2265 |
| _version_ | 1828113631875694592 |
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| author | Åse Emblem Steinar D. Johansen Ole Morten Seternes Siri Okkenhaug Ilona Urbarova Bård Ove Karlsen |
| author_facet | Åse Emblem Steinar D. Johansen Ole Morten Seternes Siri Okkenhaug Ilona Urbarova Bård Ove Karlsen |
| author_sort | Åse Emblem |
| collection | DOAJ |
| description | Marine bioprospecting is the search for new marine bioactive compounds and large-scale screening in extracts represents the traditional approach. Here, we report an alternative complementary protocol, called digital marine bioprospecting, based on deep sequencing of transcriptomes. We sequenced the transcriptomes from the adult polyp stage of two cold-water sea anemones, Bolocera tuediae and Hormathia digitata. We generated approximately 1.1 million quality-filtered sequencing reads by 454 pyrosequencing, which were assembled into approximately 120,000 contigs and 220,000 single reads. Based on annotation and gene ontology analysis we profiled the expressed mRNA transcripts according to known biological processes. As a proof-of-concept we identified polypeptide toxins with a potential blocking activity on sodium and potassium voltage-gated channels from digital transcriptome libraries. |
| first_indexed | 2024-04-11T12:11:50Z |
| format | Article |
| id | doaj.art-951eb3ad78014cbc8d9c55225a07dd82 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T12:11:50Z |
| publishDate | 2012-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-951eb3ad78014cbc8d9c55225a07dd822022-12-22T04:24:36ZengMDPI AGMarine Drugs1660-33972012-10-0110102265227910.3390/md10102265Digital Marine Bioprospecting: Mining New Neurotoxin Drug Candidates from the Transcriptomes of Cold-Water Sea AnemonesÅse EmblemSteinar D. JohansenOle Morten SeternesSiri OkkenhaugIlona UrbarovaBård Ove KarlsenMarine bioprospecting is the search for new marine bioactive compounds and large-scale screening in extracts represents the traditional approach. Here, we report an alternative complementary protocol, called digital marine bioprospecting, based on deep sequencing of transcriptomes. We sequenced the transcriptomes from the adult polyp stage of two cold-water sea anemones, Bolocera tuediae and Hormathia digitata. We generated approximately 1.1 million quality-filtered sequencing reads by 454 pyrosequencing, which were assembled into approximately 120,000 contigs and 220,000 single reads. Based on annotation and gene ontology analysis we profiled the expressed mRNA transcripts according to known biological processes. As a proof-of-concept we identified polypeptide toxins with a potential blocking activity on sodium and potassium voltage-gated channels from digital transcriptome libraries.http://www.mdpi.com/1660-3397/10/10/2265deep sequencingdrug discoverymarine bioprospectingneurotoxinsea anemonetranscriptomics |
| spellingShingle | Åse Emblem Steinar D. Johansen Ole Morten Seternes Siri Okkenhaug Ilona Urbarova Bård Ove Karlsen Digital Marine Bioprospecting: Mining New Neurotoxin Drug Candidates from the Transcriptomes of Cold-Water Sea Anemones Marine Drugs deep sequencing drug discovery marine bioprospecting neurotoxin sea anemone transcriptomics |
| title | Digital Marine Bioprospecting: Mining New Neurotoxin Drug Candidates from the Transcriptomes of Cold-Water Sea Anemones |
| title_full | Digital Marine Bioprospecting: Mining New Neurotoxin Drug Candidates from the Transcriptomes of Cold-Water Sea Anemones |
| title_fullStr | Digital Marine Bioprospecting: Mining New Neurotoxin Drug Candidates from the Transcriptomes of Cold-Water Sea Anemones |
| title_full_unstemmed | Digital Marine Bioprospecting: Mining New Neurotoxin Drug Candidates from the Transcriptomes of Cold-Water Sea Anemones |
| title_short | Digital Marine Bioprospecting: Mining New Neurotoxin Drug Candidates from the Transcriptomes of Cold-Water Sea Anemones |
| title_sort | digital marine bioprospecting mining new neurotoxin drug candidates from the transcriptomes of cold water sea anemones |
| topic | deep sequencing drug discovery marine bioprospecting neurotoxin sea anemone transcriptomics |
| url | http://www.mdpi.com/1660-3397/10/10/2265 |
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