<it>Mycobacterium tuberculosis </it>complex drug resistance pattern and identification of species causing tuberculosis in the West and Centre regions of Cameroon
<p>Abstract</p> <p>Background</p> <p>Data on the levels of resistance of <it>Mycobacterium tuberculosis </it>complex (MTBC) strains to first line anti-tuberculosis drugs in Cameroon, and on the species of MTBC circulating in the country are obsolete. The pic...
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| Format: | Article |
| Language: | English |
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BMC
2011-04-01
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| Series: | BMC Infectious Diseases |
| Online Access: | http://www.biomedcentral.com/1471-2334/11/94 |
| _version_ | 1819054975847759872 |
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| author | Tedom Jean-Claude Cho-Ngwa Fidelis Penlap Veronique B Assam-Assam Jean-Paul Ane-Anyangwe Irene Titanji Vincent P |
| author_facet | Tedom Jean-Claude Cho-Ngwa Fidelis Penlap Veronique B Assam-Assam Jean-Paul Ane-Anyangwe Irene Titanji Vincent P |
| author_sort | Tedom Jean-Claude |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Data on the levels of resistance of <it>Mycobacterium tuberculosis </it>complex (MTBC) strains to first line anti-tuberculosis drugs in Cameroon, and on the species of MTBC circulating in the country are obsolete. The picture about 10 years after the last studies, and 6 years after the re-organisation of the National Tuberculosis (TB) Control Programme (NTBCP) is not known.</p> <p>Methods</p> <p>The study was conducted from February to July 2009 in the West and Centre regions of Cameroon. A total of 756 suspected patients were studied. MTBC species were detected by the standard Ziehl-Neelsen staining method. Bacterial susceptibility to the first line drugs [isoniazid (INH), rifampicin (RIF), ethambutol (EMB) and streptomycin (SM)] were performed on cultures using the indirect proportion method. MTBC species were identified by standard biochemical and culture methods.</p> <p>Results</p> <p>Of the 756 suspected patients, 154 (20.37%) were positive by smear microscopy. Of these, 20.77% were HIV patients. The growth of <it>Mycobacterium </it>was observed with the sputa from 149 (96.75%) subjects. All the isolates were identified as either <it>M. tuberculosis </it>or <it>M. africanum</it>. Among these, 16 (10.73%) were resistant to at least one drug (13.3% for the West region and 8.1% for the Centre). The initial resistance rates were 7.35% for the Centre region and 11.29% for the West region, while the acquired resistance rates were 16.66% (1/6) for the Centre region and 23.07% (3/13) for the West. Within the two regions, the highest total resistance to one drug was obtained with INH and SM (2.68% each). Multidrug-resistance (MDR) was observed only in the West region at a rate of 6.67%. No resistance was recorded for EMB.</p> <p>Conclusions</p> <p><it>M. tuberculosis </it>and <it>M. africanum </it>remain the MTBC species causing pulmonary TB in the West and Centre regions of Cameroon. Following the re-organisation of the NTBCP, resistance to all first line anti-TB drugs has declined significantly (<it>p </it>< 0.05 for West; and <it>p </it>< 0.01 for Centre) in comparison to previous studies. However, the general rates of anti-TB drug resistance remain high in the country, underscoring the need for greater enforcement of control strategies.</p> |
| first_indexed | 2024-12-21T13:00:10Z |
| format | Article |
| id | doaj.art-952041a0e64f415c854f6bc2d306f34c |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
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| publishDate | 2011-04-01 |
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| series | BMC Infectious Diseases |
| spelling | doaj.art-952041a0e64f415c854f6bc2d306f34c2022-12-21T19:03:12ZengBMCBMC Infectious Diseases1471-23342011-04-011119410.1186/1471-2334-11-94<it>Mycobacterium tuberculosis </it>complex drug resistance pattern and identification of species causing tuberculosis in the West and Centre regions of CameroonTedom Jean-ClaudeCho-Ngwa FidelisPenlap Veronique BAssam-Assam Jean-PaulAne-Anyangwe IreneTitanji Vincent P<p>Abstract</p> <p>Background</p> <p>Data on the levels of resistance of <it>Mycobacterium tuberculosis </it>complex (MTBC) strains to first line anti-tuberculosis drugs in Cameroon, and on the species of MTBC circulating in the country are obsolete. The picture about 10 years after the last studies, and 6 years after the re-organisation of the National Tuberculosis (TB) Control Programme (NTBCP) is not known.</p> <p>Methods</p> <p>The study was conducted from February to July 2009 in the West and Centre regions of Cameroon. A total of 756 suspected patients were studied. MTBC species were detected by the standard Ziehl-Neelsen staining method. Bacterial susceptibility to the first line drugs [isoniazid (INH), rifampicin (RIF), ethambutol (EMB) and streptomycin (SM)] were performed on cultures using the indirect proportion method. MTBC species were identified by standard biochemical and culture methods.</p> <p>Results</p> <p>Of the 756 suspected patients, 154 (20.37%) were positive by smear microscopy. Of these, 20.77% were HIV patients. The growth of <it>Mycobacterium </it>was observed with the sputa from 149 (96.75%) subjects. All the isolates were identified as either <it>M. tuberculosis </it>or <it>M. africanum</it>. Among these, 16 (10.73%) were resistant to at least one drug (13.3% for the West region and 8.1% for the Centre). The initial resistance rates were 7.35% for the Centre region and 11.29% for the West region, while the acquired resistance rates were 16.66% (1/6) for the Centre region and 23.07% (3/13) for the West. Within the two regions, the highest total resistance to one drug was obtained with INH and SM (2.68% each). Multidrug-resistance (MDR) was observed only in the West region at a rate of 6.67%. No resistance was recorded for EMB.</p> <p>Conclusions</p> <p><it>M. tuberculosis </it>and <it>M. africanum </it>remain the MTBC species causing pulmonary TB in the West and Centre regions of Cameroon. Following the re-organisation of the NTBCP, resistance to all first line anti-TB drugs has declined significantly (<it>p </it>< 0.05 for West; and <it>p </it>< 0.01 for Centre) in comparison to previous studies. However, the general rates of anti-TB drug resistance remain high in the country, underscoring the need for greater enforcement of control strategies.</p>http://www.biomedcentral.com/1471-2334/11/94 |
| spellingShingle | Tedom Jean-Claude Cho-Ngwa Fidelis Penlap Veronique B Assam-Assam Jean-Paul Ane-Anyangwe Irene Titanji Vincent P <it>Mycobacterium tuberculosis </it>complex drug resistance pattern and identification of species causing tuberculosis in the West and Centre regions of Cameroon BMC Infectious Diseases |
| title | <it>Mycobacterium tuberculosis </it>complex drug resistance pattern and identification of species causing tuberculosis in the West and Centre regions of Cameroon |
| title_full | <it>Mycobacterium tuberculosis </it>complex drug resistance pattern and identification of species causing tuberculosis in the West and Centre regions of Cameroon |
| title_fullStr | <it>Mycobacterium tuberculosis </it>complex drug resistance pattern and identification of species causing tuberculosis in the West and Centre regions of Cameroon |
| title_full_unstemmed | <it>Mycobacterium tuberculosis </it>complex drug resistance pattern and identification of species causing tuberculosis in the West and Centre regions of Cameroon |
| title_short | <it>Mycobacterium tuberculosis </it>complex drug resistance pattern and identification of species causing tuberculosis in the West and Centre regions of Cameroon |
| title_sort | it mycobacterium tuberculosis it complex drug resistance pattern and identification of species causing tuberculosis in the west and centre regions of cameroon |
| url | http://www.biomedcentral.com/1471-2334/11/94 |
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