Two New Haplotypes of <i>Bartonella</i> sp. Isolated from <i>Lipoptena fortisetosa</i> (Diptera: Hippoboscidae) in SE Poland
Insects of the genus <i>Lipoptena</i> are parasitic arthropods with a broad host range. Due to the type of parasitism (hematophagy), their potential role as vectors of pathogens, i.e., <i>Bartonella</i> sp., <i>Anaplasma phagocytophilum</i>, <i>Rickettsia<...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | Insects |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4450/12/6/485 |
| _version_ | 1797532941772914688 |
|---|---|
| author | Katarzyna Bartosik Weronika Maślanko Alicja Buczek Marek Asman Joanna Witecka Ewelina Szwaj Paweł Szczepan Błaszkiewicz Magdalena Świsłocka |
| author_facet | Katarzyna Bartosik Weronika Maślanko Alicja Buczek Marek Asman Joanna Witecka Ewelina Szwaj Paweł Szczepan Błaszkiewicz Magdalena Świsłocka |
| author_sort | Katarzyna Bartosik |
| collection | DOAJ |
| description | Insects of the genus <i>Lipoptena</i> are parasitic arthropods with a broad host range. Due to the type of parasitism (hematophagy), their potential role as vectors of pathogens, i.e., <i>Bartonella</i> sp., <i>Anaplasma phagocytophilum</i>, <i>Rickettsia</i> spp., and <i>Borrelia burgdorferi</i> is considered. As the range of their occurrence has been changing dynamically in recent years and infestations of humans have increasingly been reported, these organisms are now the subject of numerous studies. Our research aimed to present the molecular characteristics of <i>Bartonella</i> sp. detected in <i>Lipoptena fortisetosa</i> parasitizing wild cervids in south-eastern Poland. Adults of <i>Lipoptena</i> spp. were collected from carcasses of roe deer and red deer between spring and autumn in 2013. The PCR method was used to detect <i>Bartonella</i> sp. in the insects. We report two new haplotypes of the <i>rpo</i>B gene of <i>Bartonella</i> sp. isolated from <i>L. fortisetosa</i> feeding on wild cervids in south-eastern Poland and the presence of this invasive ectoparasitic species in the studied area since 2013. Phylogenetic analyses of newly obtained <i>Bartonella</i> sp. haplotypes confirmed their unique position on the constructed tree and network topology. The <i>rpo</i>B gene sequences found belonging to lineage B support the view that this phylogenetic lineage represents a novel <i>Bartonella</i> species. |
| first_indexed | 2024-03-10T11:06:32Z |
| format | Article |
| id | doaj.art-9525e64cf2c648cc8388ce26363f5410 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T11:06:32Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-9525e64cf2c648cc8388ce26363f54102023-11-21T21:05:31ZengMDPI AGInsects2075-44502021-05-0112648510.3390/insects12060485Two New Haplotypes of <i>Bartonella</i> sp. Isolated from <i>Lipoptena fortisetosa</i> (Diptera: Hippoboscidae) in SE PolandKatarzyna Bartosik0Weronika Maślanko1Alicja Buczek2Marek Asman3Joanna Witecka4Ewelina Szwaj5Paweł Szczepan Błaszkiewicz6Magdalena Świsłocka7Chair and Department of Biology and Parasitology, Faculty of Health Sciences, Medical University of Lublin, Radziwiłłowska 11 St., 20-080 Lublin, PolandDepartment of Animal Ethology and Wildlife Management, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13 St., 20-950 Lublin, PolandChair and Department of Biology and Parasitology, Faculty of Health Sciences, Medical University of Lublin, Radziwiłłowska 11 St., 20-080 Lublin, PolandDepartment of Parasitology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, Jedności 8 St., 41-200 Sosnowiec, PolandDepartment of Parasitology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, Jedności 8 St., 41-200 Sosnowiec, PolandChair and Department of Biology and Parasitology, Faculty of Health Sciences, Medical University of Lublin, Radziwiłłowska 11 St., 20-080 Lublin, PolandChair and Department of Biology and Parasitology, Faculty of Health Sciences, Medical University of Lublin, Radziwiłłowska 11 St., 20-080 Lublin, PolandDepartment of Zoology and Genetics, Faculty of Biology, University of Bialystok, Ciołkowskiego 1J St., 15-245 Białystok, PolandInsects of the genus <i>Lipoptena</i> are parasitic arthropods with a broad host range. Due to the type of parasitism (hematophagy), their potential role as vectors of pathogens, i.e., <i>Bartonella</i> sp., <i>Anaplasma phagocytophilum</i>, <i>Rickettsia</i> spp., and <i>Borrelia burgdorferi</i> is considered. As the range of their occurrence has been changing dynamically in recent years and infestations of humans have increasingly been reported, these organisms are now the subject of numerous studies. Our research aimed to present the molecular characteristics of <i>Bartonella</i> sp. detected in <i>Lipoptena fortisetosa</i> parasitizing wild cervids in south-eastern Poland. Adults of <i>Lipoptena</i> spp. were collected from carcasses of roe deer and red deer between spring and autumn in 2013. The PCR method was used to detect <i>Bartonella</i> sp. in the insects. We report two new haplotypes of the <i>rpo</i>B gene of <i>Bartonella</i> sp. isolated from <i>L. fortisetosa</i> feeding on wild cervids in south-eastern Poland and the presence of this invasive ectoparasitic species in the studied area since 2013. Phylogenetic analyses of newly obtained <i>Bartonella</i> sp. haplotypes confirmed their unique position on the constructed tree and network topology. The <i>rpo</i>B gene sequences found belonging to lineage B support the view that this phylogenetic lineage represents a novel <i>Bartonella</i> species.https://www.mdpi.com/2075-4450/12/6/485<i>Lipoptena</i> sp.deer kedsinvasive species<i>Cervus elaphus</i>ectoparasiteswild cervids |
| spellingShingle | Katarzyna Bartosik Weronika Maślanko Alicja Buczek Marek Asman Joanna Witecka Ewelina Szwaj Paweł Szczepan Błaszkiewicz Magdalena Świsłocka Two New Haplotypes of <i>Bartonella</i> sp. Isolated from <i>Lipoptena fortisetosa</i> (Diptera: Hippoboscidae) in SE Poland Insects <i>Lipoptena</i> sp. deer keds invasive species <i>Cervus elaphus</i> ectoparasites wild cervids |
| title | Two New Haplotypes of <i>Bartonella</i> sp. Isolated from <i>Lipoptena fortisetosa</i> (Diptera: Hippoboscidae) in SE Poland |
| title_full | Two New Haplotypes of <i>Bartonella</i> sp. Isolated from <i>Lipoptena fortisetosa</i> (Diptera: Hippoboscidae) in SE Poland |
| title_fullStr | Two New Haplotypes of <i>Bartonella</i> sp. Isolated from <i>Lipoptena fortisetosa</i> (Diptera: Hippoboscidae) in SE Poland |
| title_full_unstemmed | Two New Haplotypes of <i>Bartonella</i> sp. Isolated from <i>Lipoptena fortisetosa</i> (Diptera: Hippoboscidae) in SE Poland |
| title_short | Two New Haplotypes of <i>Bartonella</i> sp. Isolated from <i>Lipoptena fortisetosa</i> (Diptera: Hippoboscidae) in SE Poland |
| title_sort | two new haplotypes of i bartonella i sp isolated from i lipoptena fortisetosa i diptera hippoboscidae in se poland |
| topic | <i>Lipoptena</i> sp. deer keds invasive species <i>Cervus elaphus</i> ectoparasites wild cervids |
| url | https://www.mdpi.com/2075-4450/12/6/485 |
| work_keys_str_mv | AT katarzynabartosik twonewhaplotypesofibartonellaispisolatedfromilipoptenafortisetosaidipterahippoboscidaeinsepoland AT weronikamaslanko twonewhaplotypesofibartonellaispisolatedfromilipoptenafortisetosaidipterahippoboscidaeinsepoland AT alicjabuczek twonewhaplotypesofibartonellaispisolatedfromilipoptenafortisetosaidipterahippoboscidaeinsepoland AT marekasman twonewhaplotypesofibartonellaispisolatedfromilipoptenafortisetosaidipterahippoboscidaeinsepoland AT joannawitecka twonewhaplotypesofibartonellaispisolatedfromilipoptenafortisetosaidipterahippoboscidaeinsepoland AT ewelinaszwaj twonewhaplotypesofibartonellaispisolatedfromilipoptenafortisetosaidipterahippoboscidaeinsepoland AT pawełszczepanbłaszkiewicz twonewhaplotypesofibartonellaispisolatedfromilipoptenafortisetosaidipterahippoboscidaeinsepoland AT magdalenaswisłocka twonewhaplotypesofibartonellaispisolatedfromilipoptenafortisetosaidipterahippoboscidaeinsepoland |