Cytoprotective, Antiproliferative, and Anti-Oxidant Potential of the Hydroethanolic Extract of <i>Fridericia chica</i> Leaves on Human Cancer Cell Lines Exposed to α- and β-Zearalenol
<i>Fridericia chica</i> (Bignoniaceae) is a Colombian Caribbean plant with numerous health benefits, including properties such as wound healing, immune system stimulation, and antioxidant capacity, among others. Mycotoxins alpha-zearalenol (α-ZEL) and beta-zearalenol (β-ZEL) are phase I...
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MDPI AG
2023-01-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/15/1/36 |
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| author | Neda Alvarez-Ortega Karina Caballero-Gallardo Cristina Juan Ana Juan-Garcia Jesus Olivero-Verbel |
| author_facet | Neda Alvarez-Ortega Karina Caballero-Gallardo Cristina Juan Ana Juan-Garcia Jesus Olivero-Verbel |
| author_sort | Neda Alvarez-Ortega |
| collection | DOAJ |
| description | <i>Fridericia chica</i> (Bignoniaceae) is a Colombian Caribbean plant with numerous health benefits, including properties such as wound healing, immune system stimulation, and antioxidant capacity, among others. Mycotoxins alpha-zearalenol (α-ZEL) and beta-zearalenol (β-ZEL) are phase I metabolites of zearalenone, a natural product involved in endocrine disruption and cell proliferation processes. This study aimed to investigate the cytotoxic potential of the hydroethanolic extract of <i>F. chica</i> leaves (HEFc) and determine their protective effects against proliferation induced by α-ZEL and β-ZEL on human hepatoma HepG2, lung cancer Calu-1, and primary normal human epidermal keratinocytes, neonatal (HEKn). The cytotoxicity of HEFc was measured in a range from 4 to 1000 µg/mL and from 0.4 to 100 μM for both α-ZEL and β-ZEL. Cell production of intracellular ROS was monitored using the H<sub>2</sub>-DCFDA probe. The cells exposed to HEFc presented IC<sub>50</sub> of 128, 249, and 602 µg/mL for the HepG2, Calu-1, and HEKn cells, respectively. A greater selectivity was seen in HepG2 cells [selectivity index (SI) = 3.5] than in Calu-1 cells (SI = 2.4). Cells treated with mycotoxins remained viable during the first day, and cell proliferation increased at low tested concentrations (0.4-6.3 µM) in all three cell lines. However, after 48 h treatment, cells exposed to 50 and 100 µM of α-ZEL and β-ZEL displayed decreased viability. HEFc at 16 µg/mL was able to give some protection against cytotoxicity induced by high concentrations of β-ZEL in HepG2, reducing also cell proliferation elicited at low levels of α-ZEL and β-ZEL. ROS production was not observed in cells treated with this HEFc concentration; however, it prevented ROS formation induced by treatment with 50 µM α-ZEL or β-ZEL. In summary, HEFc isolated from plants grown in northern Colombia displayed promising results against cell proliferation and oxidative stress caused by mycotoxins. |
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| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T11:06:17Z |
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| spelling | doaj.art-9526445f99ef4cceb0b203386dee0b922023-12-01T00:56:52ZengMDPI AGToxins2072-66512023-01-011513610.3390/toxins15010036Cytoprotective, Antiproliferative, and Anti-Oxidant Potential of the Hydroethanolic Extract of <i>Fridericia chica</i> Leaves on Human Cancer Cell Lines Exposed to α- and β-ZearalenolNeda Alvarez-Ortega0Karina Caballero-Gallardo1Cristina Juan2Ana Juan-Garcia3Jesus Olivero-Verbel4Environmental and Computational Chemistry Group, School of Pharmaceutical Sciences, Zaragocilla Campus, University of Cartagena, Cartagena 130014, ColombiaEnvironmental and Computational Chemistry Group, School of Pharmaceutical Sciences, Zaragocilla Campus, University of Cartagena, Cartagena 130014, ColombiaLaboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia (Spain)—Avda, Vicent Andrés Estellés, s/n. Burjassot, 46100 València, SpainLaboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia (Spain)—Avda, Vicent Andrés Estellés, s/n. Burjassot, 46100 València, SpainEnvironmental and Computational Chemistry Group, School of Pharmaceutical Sciences, Zaragocilla Campus, University of Cartagena, Cartagena 130014, Colombia<i>Fridericia chica</i> (Bignoniaceae) is a Colombian Caribbean plant with numerous health benefits, including properties such as wound healing, immune system stimulation, and antioxidant capacity, among others. Mycotoxins alpha-zearalenol (α-ZEL) and beta-zearalenol (β-ZEL) are phase I metabolites of zearalenone, a natural product involved in endocrine disruption and cell proliferation processes. This study aimed to investigate the cytotoxic potential of the hydroethanolic extract of <i>F. chica</i> leaves (HEFc) and determine their protective effects against proliferation induced by α-ZEL and β-ZEL on human hepatoma HepG2, lung cancer Calu-1, and primary normal human epidermal keratinocytes, neonatal (HEKn). The cytotoxicity of HEFc was measured in a range from 4 to 1000 µg/mL and from 0.4 to 100 μM for both α-ZEL and β-ZEL. Cell production of intracellular ROS was monitored using the H<sub>2</sub>-DCFDA probe. The cells exposed to HEFc presented IC<sub>50</sub> of 128, 249, and 602 µg/mL for the HepG2, Calu-1, and HEKn cells, respectively. A greater selectivity was seen in HepG2 cells [selectivity index (SI) = 3.5] than in Calu-1 cells (SI = 2.4). Cells treated with mycotoxins remained viable during the first day, and cell proliferation increased at low tested concentrations (0.4-6.3 µM) in all three cell lines. However, after 48 h treatment, cells exposed to 50 and 100 µM of α-ZEL and β-ZEL displayed decreased viability. HEFc at 16 µg/mL was able to give some protection against cytotoxicity induced by high concentrations of β-ZEL in HepG2, reducing also cell proliferation elicited at low levels of α-ZEL and β-ZEL. ROS production was not observed in cells treated with this HEFc concentration; however, it prevented ROS formation induced by treatment with 50 µM α-ZEL or β-ZEL. In summary, HEFc isolated from plants grown in northern Colombia displayed promising results against cell proliferation and oxidative stress caused by mycotoxins.https://www.mdpi.com/2072-6651/15/1/36mycotoxinsROScytotoxicityplant extracts |
| spellingShingle | Neda Alvarez-Ortega Karina Caballero-Gallardo Cristina Juan Ana Juan-Garcia Jesus Olivero-Verbel Cytoprotective, Antiproliferative, and Anti-Oxidant Potential of the Hydroethanolic Extract of <i>Fridericia chica</i> Leaves on Human Cancer Cell Lines Exposed to α- and β-Zearalenol Toxins mycotoxins ROS cytotoxicity plant extracts |
| title | Cytoprotective, Antiproliferative, and Anti-Oxidant Potential of the Hydroethanolic Extract of <i>Fridericia chica</i> Leaves on Human Cancer Cell Lines Exposed to α- and β-Zearalenol |
| title_full | Cytoprotective, Antiproliferative, and Anti-Oxidant Potential of the Hydroethanolic Extract of <i>Fridericia chica</i> Leaves on Human Cancer Cell Lines Exposed to α- and β-Zearalenol |
| title_fullStr | Cytoprotective, Antiproliferative, and Anti-Oxidant Potential of the Hydroethanolic Extract of <i>Fridericia chica</i> Leaves on Human Cancer Cell Lines Exposed to α- and β-Zearalenol |
| title_full_unstemmed | Cytoprotective, Antiproliferative, and Anti-Oxidant Potential of the Hydroethanolic Extract of <i>Fridericia chica</i> Leaves on Human Cancer Cell Lines Exposed to α- and β-Zearalenol |
| title_short | Cytoprotective, Antiproliferative, and Anti-Oxidant Potential of the Hydroethanolic Extract of <i>Fridericia chica</i> Leaves on Human Cancer Cell Lines Exposed to α- and β-Zearalenol |
| title_sort | cytoprotective antiproliferative and anti oxidant potential of the hydroethanolic extract of i fridericia chica i leaves on human cancer cell lines exposed to α and β zearalenol |
| topic | mycotoxins ROS cytotoxicity plant extracts |
| url | https://www.mdpi.com/2072-6651/15/1/36 |
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