Monolayer/Bilayer Equilibrium of Phospholipids in Gel or Liquid States: Interfacial Adsorption via Monomer or Liposome Diffusion?
Phospholipids (PLs) are widely used in the pharma industry and a better understanding of their behavior under different conditions is helpful for applications such as their use as medical transporters. The transition temperature T<sub>m</sub> affects the lipid conformation and the interf...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Online Access: | https://www.mdpi.com/2310-2861/9/10/803 |
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| author | Kirsten Ullmann Lea Fachet Hermann Nirschl Gero Leneweit |
| author_facet | Kirsten Ullmann Lea Fachet Hermann Nirschl Gero Leneweit |
| author_sort | Kirsten Ullmann |
| collection | DOAJ |
| description | Phospholipids (PLs) are widely used in the pharma industry and a better understanding of their behavior under different conditions is helpful for applications such as their use as medical transporters. The transition temperature T<sub>m</sub> affects the lipid conformation and the interfacial tension between <b>p</b>er<b>f</b>luoroperhydro<b>p</b>henanthrene (PFP) and an aqueous suspension of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC), 1,2-distearoyl-sn-glycero-3-phosphatidylcholine (DSPC), as well as a mixture of these PLs with cholesterol. Interfacial tensions were measured with the Du Noüy ring at quasi-equilibrium; the area per molecule was calculated according to the Gibbsian approach and a time-dependent tension gradient. Results show that the time <i>t<sub>ε</sub></i> to reach quasi-equilibrium was shorter when the temperature was above T<sub>m</sub>, indicating a faster adsorption process (<i>t<sub>ε</sub></i><sub>,DPPC</sub>,<sub>36 °C</sub> = 48 h, <i>t<sub>ε</sub></i><sub>,DPPC,48 °C</sub> = 24 h) for PL in the liquid crystalline state than in the gel state (T < T<sub>m</sub>). In addition, concentration-dependent results of the interfacial tension revealed that above the respective T<sub>m</sub> and at all concentrations <i>c</i> > 0.1 mM, the average minimum interfacial tension for DPPC and DSPC (14.1 mN/m and 15.3 mN/m) does not differ significantly between those two lipids. Equilibrium between monolayers and bilayers shows that for T < T<sub>m</sub>, surface pressures <i>∏</i> ≈ 31 mN/m are reached while for T > T<sub>m</sub>, <i>∏</i> ≈ 41 mN/m. Mixtures with cholesterol only reach <i>∏</i> ≤ 31 mN/m T<sub>m</sub>, with no significant difference between the two PLs. The higher interfacial tension of the mixture indicates stabilization of the liposomal conformation in the aqueous phase by the addition of cholesterol. The high diffusion coefficients show that adsorption is mainly based on liposomes. |
| first_indexed | 2024-03-10T21:13:55Z |
| format | Article |
| id | doaj.art-952c59913d9a4260a02839a2aed8cd52 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-10T21:13:55Z |
| publishDate | 2023-10-01 |
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| spelling | doaj.art-952c59913d9a4260a02839a2aed8cd522023-11-19T16:35:35ZengMDPI AGGels2310-28612023-10-0191080310.3390/gels9100803Monolayer/Bilayer Equilibrium of Phospholipids in Gel or Liquid States: Interfacial Adsorption via Monomer or Liposome Diffusion?Kirsten Ullmann0Lea Fachet1Hermann Nirschl2Gero Leneweit3Karlsruhe Institute of Technology (KIT), Department of Mechanical Process Engineering and Mechanics, Straße am Forum 8, 76131 Karlsruhe, GermanyIndependent Researcher, 76131 Karlsruhe, GermanyKarlsruhe Institute of Technology (KIT), Department of Mechanical Process Engineering and Mechanics, Straße am Forum 8, 76131 Karlsruhe, GermanyCarl Gustav Carus-Institute, Association for the Promotion of Cancer Therapy, Allmendstr. 55, 75223 Niefern-Oeschelbronn, GermanyPhospholipids (PLs) are widely used in the pharma industry and a better understanding of their behavior under different conditions is helpful for applications such as their use as medical transporters. The transition temperature T<sub>m</sub> affects the lipid conformation and the interfacial tension between <b>p</b>er<b>f</b>luoroperhydro<b>p</b>henanthrene (PFP) and an aqueous suspension of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC), 1,2-distearoyl-sn-glycero-3-phosphatidylcholine (DSPC), as well as a mixture of these PLs with cholesterol. Interfacial tensions were measured with the Du Noüy ring at quasi-equilibrium; the area per molecule was calculated according to the Gibbsian approach and a time-dependent tension gradient. Results show that the time <i>t<sub>ε</sub></i> to reach quasi-equilibrium was shorter when the temperature was above T<sub>m</sub>, indicating a faster adsorption process (<i>t<sub>ε</sub></i><sub>,DPPC</sub>,<sub>36 °C</sub> = 48 h, <i>t<sub>ε</sub></i><sub>,DPPC,48 °C</sub> = 24 h) for PL in the liquid crystalline state than in the gel state (T < T<sub>m</sub>). In addition, concentration-dependent results of the interfacial tension revealed that above the respective T<sub>m</sub> and at all concentrations <i>c</i> > 0.1 mM, the average minimum interfacial tension for DPPC and DSPC (14.1 mN/m and 15.3 mN/m) does not differ significantly between those two lipids. Equilibrium between monolayers and bilayers shows that for T < T<sub>m</sub>, surface pressures <i>∏</i> ≈ 31 mN/m are reached while for T > T<sub>m</sub>, <i>∏</i> ≈ 41 mN/m. Mixtures with cholesterol only reach <i>∏</i> ≤ 31 mN/m T<sub>m</sub>, with no significant difference between the two PLs. The higher interfacial tension of the mixture indicates stabilization of the liposomal conformation in the aqueous phase by the addition of cholesterol. The high diffusion coefficients show that adsorption is mainly based on liposomes.https://www.mdpi.com/2310-2861/9/10/803phospholipidsmonomer and liposomal diffusionperfluorocarboninterfacial tensiontensiometrytemperature |
| spellingShingle | Kirsten Ullmann Lea Fachet Hermann Nirschl Gero Leneweit Monolayer/Bilayer Equilibrium of Phospholipids in Gel or Liquid States: Interfacial Adsorption via Monomer or Liposome Diffusion? Gels phospholipids monomer and liposomal diffusion perfluorocarbon interfacial tension tensiometry temperature |
| title | Monolayer/Bilayer Equilibrium of Phospholipids in Gel or Liquid States: Interfacial Adsorption via Monomer or Liposome Diffusion? |
| title_full | Monolayer/Bilayer Equilibrium of Phospholipids in Gel or Liquid States: Interfacial Adsorption via Monomer or Liposome Diffusion? |
| title_fullStr | Monolayer/Bilayer Equilibrium of Phospholipids in Gel or Liquid States: Interfacial Adsorption via Monomer or Liposome Diffusion? |
| title_full_unstemmed | Monolayer/Bilayer Equilibrium of Phospholipids in Gel or Liquid States: Interfacial Adsorption via Monomer or Liposome Diffusion? |
| title_short | Monolayer/Bilayer Equilibrium of Phospholipids in Gel or Liquid States: Interfacial Adsorption via Monomer or Liposome Diffusion? |
| title_sort | monolayer bilayer equilibrium of phospholipids in gel or liquid states interfacial adsorption via monomer or liposome diffusion |
| topic | phospholipids monomer and liposomal diffusion perfluorocarbon interfacial tension tensiometry temperature |
| url | https://www.mdpi.com/2310-2861/9/10/803 |
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