Transcriptomic Adjustments of Staphylococcus aureus COL (MRSA) Forming Biofilms Under Acidic and Alkaline Conditions
Methicillin-resistant Staphylococcus aureus (MRSA) strains are important human pathogens and a significant health hazard for hospitals and the food industry. They are resistant to β-lactam antibiotics including methicillin and extremely difficult to treat. In this study, we show that the Staphylococ...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-10-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02393/full |
| _version_ | 1828201526524379136 |
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| author | Georgios Efthimiou George Tsiamis Milton A. Typas Katherine M. Pappas |
| author_facet | Georgios Efthimiou George Tsiamis Milton A. Typas Katherine M. Pappas |
| author_sort | Georgios Efthimiou |
| collection | DOAJ |
| description | Methicillin-resistant Staphylococcus aureus (MRSA) strains are important human pathogens and a significant health hazard for hospitals and the food industry. They are resistant to β-lactam antibiotics including methicillin and extremely difficult to treat. In this study, we show that the Staphylococcus aureus COL (MRSA) strain, with a known complete genome, can easily survive and grow under acidic and alkaline conditions (pH5 and pH9, respectively), both planktonically and as a biofilm. A microarray-based analysis of both planktonic and biofilm cells was performed under acidic and alkaline conditions showing that several genes are up- or down-regulated under different environmental conditions and growth modes. These genes were coding for transcription regulators, ion transporters, cell wall biosynthetic enzymes, autolytic enzymes, adhesion proteins and antibiotic resistance factors, most of which are associated with biofilm formation. These results will facilitate a better understanding of the physiological adjustments occurring in biofilm-associated S. aureus COL cells growing in acidic or alkaline environments, which will enable the development of new efficient treatment or disinfection strategies. |
| first_indexed | 2024-04-12T11:31:19Z |
| format | Article |
| id | doaj.art-952d32e6f237472f8db8b75106daef2a |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T11:31:19Z |
| publishDate | 2019-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-952d32e6f237472f8db8b75106daef2a2022-12-22T03:35:00ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-10-011010.3389/fmicb.2019.02393485826Transcriptomic Adjustments of Staphylococcus aureus COL (MRSA) Forming Biofilms Under Acidic and Alkaline ConditionsGeorgios Efthimiou0George Tsiamis1Milton A. Typas2Katherine M. Pappas3Department of Genetics and Biotechnology, Faculty of Biology, National and Kapodistrian University of Athens, Athens, GreeceDepartment of Environmental Engineering, University of Patras, Agrinio, GreeceDepartment of Genetics and Biotechnology, Faculty of Biology, National and Kapodistrian University of Athens, Athens, GreeceDepartment of Genetics and Biotechnology, Faculty of Biology, National and Kapodistrian University of Athens, Athens, GreeceMethicillin-resistant Staphylococcus aureus (MRSA) strains are important human pathogens and a significant health hazard for hospitals and the food industry. They are resistant to β-lactam antibiotics including methicillin and extremely difficult to treat. In this study, we show that the Staphylococcus aureus COL (MRSA) strain, with a known complete genome, can easily survive and grow under acidic and alkaline conditions (pH5 and pH9, respectively), both planktonically and as a biofilm. A microarray-based analysis of both planktonic and biofilm cells was performed under acidic and alkaline conditions showing that several genes are up- or down-regulated under different environmental conditions and growth modes. These genes were coding for transcription regulators, ion transporters, cell wall biosynthetic enzymes, autolytic enzymes, adhesion proteins and antibiotic resistance factors, most of which are associated with biofilm formation. These results will facilitate a better understanding of the physiological adjustments occurring in biofilm-associated S. aureus COL cells growing in acidic or alkaline environments, which will enable the development of new efficient treatment or disinfection strategies.https://www.frontiersin.org/article/10.3389/fmicb.2019.02393/fullMRSAbiofilmalkalineacidicmicroarraytranscription factors |
| spellingShingle | Georgios Efthimiou George Tsiamis Milton A. Typas Katherine M. Pappas Transcriptomic Adjustments of Staphylococcus aureus COL (MRSA) Forming Biofilms Under Acidic and Alkaline Conditions Frontiers in Microbiology MRSA biofilm alkaline acidic microarray transcription factors |
| title | Transcriptomic Adjustments of Staphylococcus aureus COL (MRSA) Forming Biofilms Under Acidic and Alkaline Conditions |
| title_full | Transcriptomic Adjustments of Staphylococcus aureus COL (MRSA) Forming Biofilms Under Acidic and Alkaline Conditions |
| title_fullStr | Transcriptomic Adjustments of Staphylococcus aureus COL (MRSA) Forming Biofilms Under Acidic and Alkaline Conditions |
| title_full_unstemmed | Transcriptomic Adjustments of Staphylococcus aureus COL (MRSA) Forming Biofilms Under Acidic and Alkaline Conditions |
| title_short | Transcriptomic Adjustments of Staphylococcus aureus COL (MRSA) Forming Biofilms Under Acidic and Alkaline Conditions |
| title_sort | transcriptomic adjustments of staphylococcus aureus col mrsa forming biofilms under acidic and alkaline conditions |
| topic | MRSA biofilm alkaline acidic microarray transcription factors |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.02393/full |
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