Protective effect of Buddha’s Temple extract against tert-butyl hydroperoxide stimulation-induced oxidative stress in DF-1 cells
Objective This study aimed to determine the protective efficacy of Buddha’s Temple (BT) extract against tert-butyl hydroperoxide (t-BHP)-induced oxidative stress in Gallus gallus chicken embryo fibroblast cell line (DF-1) and its effects on the cell lipid metabolism. Methods In this experimental stu...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2023-07-01
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| Series: | Animal Bioscience |
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| Online Access: | http://www.animbiosci.org/upload/pdf/ab-23-0014.pdf |
| _version_ | 1811153959595802624 |
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| author | Eun Hye Park Sung-Jo Kim |
| author_facet | Eun Hye Park Sung-Jo Kim |
| author_sort | Eun Hye Park |
| collection | DOAJ |
| description | Objective This study aimed to determine the protective efficacy of Buddha’s Temple (BT) extract against tert-butyl hydroperoxide (t-BHP)-induced oxidative stress in Gallus gallus chicken embryo fibroblast cell line (DF-1) and its effects on the cell lipid metabolism. Methods In this experimental study, Gallus gallus DF-1 fibroblast cells were pretreated with BT 10−7 for 24 hours, followed by their six-hour exposure to t-BHP (100 μM). Water-soluble tetrazolium salt-8 (WST-8) assays were performed, and the growth curve was computed. The intracellular gene expression changes caused by BT extract were confirmed through quantitative polymerase chain reaction (qPCR). Flow cytometry, oil red O staining experiment, and thin-layer chromatography were performed for the detection of intracellular metabolic mechanism changes. Results The WST-8 assay results showed that the BT pretreatment of Gallus gallus DF-1 fibroblast cell increased their cell survival rate by 1.08%±0.04%, decreased the reactive oxygen species (ROS) level by 0.93%±0.12% even after exposure to oxidants, and stabilized mitochondrial activity by 1.37%±0.36%. In addition, qPCR results confirmed that the gene expression levels of tumor necrosis factor α (TNFα), TIR domain-containing adapter inducing IFN-beta (TICAM1), and glucose-regulated protein 78 (GRP78) were regulated, which contributed to cell stabilization. Thin-layer chromatography and oil red O analyses showed a clear decrease in the contents of lipid metabolites such as triacylglycerol and free fatty acids. Conclusion In this study, we confirmed that the examined BT extract exerted selective protective effects on Gallus gallus DF-1 fibroblast cells against cell damage caused by t-BHP, which is a strong oxidative inducer. Furthermore, we established that this extract significantly reduced the intracellular ROS accumulation due to oxidative stress, which contributes to an increase in poultry production and higher incomes. |
| first_indexed | 2024-03-13T01:19:38Z |
| format | Article |
| id | doaj.art-952dcf6c106c40a8bdb44ba1a4cd605b |
| institution | Directory Open Access Journal |
| issn | 2765-0189 2765-0235 |
| language | English |
| last_indexed | 2024-03-13T01:19:38Z |
| publishDate | 2023-07-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj.art-952dcf6c106c40a8bdb44ba1a4cd605b2023-07-05T04:51:53ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352023-07-013671120112910.5713/ab.23.001425026Protective effect of Buddha’s Temple extract against tert-butyl hydroperoxide stimulation-induced oxidative stress in DF-1 cellsEun Hye Park0Sung-Jo Kim1 Division of Cosmetics and Biotechnology, College of Life and Health Sciences, Hoseo University, Baebang, Asan 31499, Korea Division of Cosmetics and Biotechnology, College of Life and Health Sciences, Hoseo University, Baebang, Asan 31499, KoreaObjective This study aimed to determine the protective efficacy of Buddha’s Temple (BT) extract against tert-butyl hydroperoxide (t-BHP)-induced oxidative stress in Gallus gallus chicken embryo fibroblast cell line (DF-1) and its effects on the cell lipid metabolism. Methods In this experimental study, Gallus gallus DF-1 fibroblast cells were pretreated with BT 10−7 for 24 hours, followed by their six-hour exposure to t-BHP (100 μM). Water-soluble tetrazolium salt-8 (WST-8) assays were performed, and the growth curve was computed. The intracellular gene expression changes caused by BT extract were confirmed through quantitative polymerase chain reaction (qPCR). Flow cytometry, oil red O staining experiment, and thin-layer chromatography were performed for the detection of intracellular metabolic mechanism changes. Results The WST-8 assay results showed that the BT pretreatment of Gallus gallus DF-1 fibroblast cell increased their cell survival rate by 1.08%±0.04%, decreased the reactive oxygen species (ROS) level by 0.93%±0.12% even after exposure to oxidants, and stabilized mitochondrial activity by 1.37%±0.36%. In addition, qPCR results confirmed that the gene expression levels of tumor necrosis factor α (TNFα), TIR domain-containing adapter inducing IFN-beta (TICAM1), and glucose-regulated protein 78 (GRP78) were regulated, which contributed to cell stabilization. Thin-layer chromatography and oil red O analyses showed a clear decrease in the contents of lipid metabolites such as triacylglycerol and free fatty acids. Conclusion In this study, we confirmed that the examined BT extract exerted selective protective effects on Gallus gallus DF-1 fibroblast cells against cell damage caused by t-BHP, which is a strong oxidative inducer. Furthermore, we established that this extract significantly reduced the intracellular ROS accumulation due to oxidative stress, which contributes to an increase in poultry production and higher incomes.http://www.animbiosci.org/upload/pdf/ab-23-0014.pdfantioxidantbuddha’s templecytoprotectivefree fatty acid df-1 fibroblast cellreactive oxygen speciestert-butyl hydroperoxide (t-bhp) |
| spellingShingle | Eun Hye Park Sung-Jo Kim Protective effect of Buddha’s Temple extract against tert-butyl hydroperoxide stimulation-induced oxidative stress in DF-1 cells Animal Bioscience antioxidant buddha’s temple cytoprotective free fatty acid df-1 fibroblast cell reactive oxygen species tert-butyl hydroperoxide (t-bhp) |
| title | Protective effect of Buddha’s Temple extract against tert-butyl hydroperoxide stimulation-induced oxidative stress in DF-1 cells |
| title_full | Protective effect of Buddha’s Temple extract against tert-butyl hydroperoxide stimulation-induced oxidative stress in DF-1 cells |
| title_fullStr | Protective effect of Buddha’s Temple extract against tert-butyl hydroperoxide stimulation-induced oxidative stress in DF-1 cells |
| title_full_unstemmed | Protective effect of Buddha’s Temple extract against tert-butyl hydroperoxide stimulation-induced oxidative stress in DF-1 cells |
| title_short | Protective effect of Buddha’s Temple extract against tert-butyl hydroperoxide stimulation-induced oxidative stress in DF-1 cells |
| title_sort | protective effect of buddha s temple extract against tert butyl hydroperoxide stimulation induced oxidative stress in df 1 cells |
| topic | antioxidant buddha’s temple cytoprotective free fatty acid df-1 fibroblast cell reactive oxygen species tert-butyl hydroperoxide (t-bhp) |
| url | http://www.animbiosci.org/upload/pdf/ab-23-0014.pdf |
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