Quality of sputum in the performance of polymerase chain reaction for diagnosis of pulmonary tuberculosis
Setting: faster alternative techniques are required to improve the diagnosis and control of pulmonary tuberculosis. Objective: To evaluate the sample quality in the performance of PCR for diagnosis of pulmonary tuberculosis. Method: During one year, sputum samples were collected from 72 pulmonary tu...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2010-01-01
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| Series: | Brazilian Journal of Infectious Diseases |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1413867010700222 |
| _version_ | 1818854199550541824 |
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| author | Rosemeri Maurici da Silva Maria Luiza Bazzo Mariana Chagas |
| author_facet | Rosemeri Maurici da Silva Maria Luiza Bazzo Mariana Chagas |
| author_sort | Rosemeri Maurici da Silva |
| collection | DOAJ |
| description | Setting: faster alternative techniques are required to improve the diagnosis and control of pulmonary tuberculosis. Objective: To evaluate the sample quality in the performance of PCR for diagnosis of pulmonary tuberculosis. Method: During one year, sputum samples were collected from 72 pulmonary tuberculosis patients and 12 non-tuberculosis controls, which were admitted to the Nereu Ramos hospital, Florianópolis city, Brazil. The samples were subjected to Ziehl-Neelsen-stained sputum smear microscopy and Lowestein-Jensen medium culture, which were defined as gold standard tests for mycobacteria, and polymerase chain reaction (PCR). Those samples that presented more than 40% of viable cells and less than 25% of epithelial cells were defined as high quality samples. Results: PCR showed sensitivity of 55.6%, specificity of 41.7%, positive predictive value of 85.1%, negative predictive value of 13.5%, and accuracy of 53.6%. High quality samples showed sensitivity of 72.4%, specificity of 50%, positive predictive value of 91.3%, negative predictive value of 20%, and accuracy of 69.7%. Low quality samples showed sensitivity of 44.2%, specificity of 37.5%, positive predictive value of 79.2%, negative predictive value of 11.1%, and accuracy of 43.1%. Conclusion: use of high quality samples improved significantly the PCR performance, especially on their sensitivity and positive predictive values. Keywords: tuberculosis, sputum, polymerase chain reaction |
| first_indexed | 2024-12-19T07:48:55Z |
| format | Article |
| id | doaj.art-952e9ca37f3d4b2abcddf44800eb072f |
| institution | Directory Open Access Journal |
| issn | 1413-8670 |
| language | English |
| last_indexed | 2024-12-19T07:48:55Z |
| publishDate | 2010-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-952e9ca37f3d4b2abcddf44800eb072f2022-12-21T20:30:14ZengElsevierBrazilian Journal of Infectious Diseases1413-86702010-01-01141116120Quality of sputum in the performance of polymerase chain reaction for diagnosis of pulmonary tuberculosisRosemeri Maurici da Silva0Maria Luiza Bazzo1Mariana Chagas2Pneumologist, Master in Medical Sciences and Doctor in Pneumology. Professor of the Medical School of the Universidade Federal de Santa Catarina and Universidade do Sul de Santa Catarina. Coordinator of the Program of Master's Degree in Health Sciences of the Universidade do Sul de Santa Catarina; Correspondence to: Rua Moçambique, 852, Rio Vermelho, Florianópolis – SC CEP: 88060415.Doctor in Microbiology and Professor of the Universidade Federal de Santa CatarinaMaster in Pharmacy, Universidade Federal de Santa CatarinaSetting: faster alternative techniques are required to improve the diagnosis and control of pulmonary tuberculosis. Objective: To evaluate the sample quality in the performance of PCR for diagnosis of pulmonary tuberculosis. Method: During one year, sputum samples were collected from 72 pulmonary tuberculosis patients and 12 non-tuberculosis controls, which were admitted to the Nereu Ramos hospital, Florianópolis city, Brazil. The samples were subjected to Ziehl-Neelsen-stained sputum smear microscopy and Lowestein-Jensen medium culture, which were defined as gold standard tests for mycobacteria, and polymerase chain reaction (PCR). Those samples that presented more than 40% of viable cells and less than 25% of epithelial cells were defined as high quality samples. Results: PCR showed sensitivity of 55.6%, specificity of 41.7%, positive predictive value of 85.1%, negative predictive value of 13.5%, and accuracy of 53.6%. High quality samples showed sensitivity of 72.4%, specificity of 50%, positive predictive value of 91.3%, negative predictive value of 20%, and accuracy of 69.7%. Low quality samples showed sensitivity of 44.2%, specificity of 37.5%, positive predictive value of 79.2%, negative predictive value of 11.1%, and accuracy of 43.1%. Conclusion: use of high quality samples improved significantly the PCR performance, especially on their sensitivity and positive predictive values. Keywords: tuberculosis, sputum, polymerase chain reactionhttp://www.sciencedirect.com/science/article/pii/S1413867010700222 |
| spellingShingle | Rosemeri Maurici da Silva Maria Luiza Bazzo Mariana Chagas Quality of sputum in the performance of polymerase chain reaction for diagnosis of pulmonary tuberculosis Brazilian Journal of Infectious Diseases |
| title | Quality of sputum in the performance of polymerase chain reaction for diagnosis of pulmonary tuberculosis |
| title_full | Quality of sputum in the performance of polymerase chain reaction for diagnosis of pulmonary tuberculosis |
| title_fullStr | Quality of sputum in the performance of polymerase chain reaction for diagnosis of pulmonary tuberculosis |
| title_full_unstemmed | Quality of sputum in the performance of polymerase chain reaction for diagnosis of pulmonary tuberculosis |
| title_short | Quality of sputum in the performance of polymerase chain reaction for diagnosis of pulmonary tuberculosis |
| title_sort | quality of sputum in the performance of polymerase chain reaction for diagnosis of pulmonary tuberculosis |
| url | http://www.sciencedirect.com/science/article/pii/S1413867010700222 |
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