Survival of a microbial inoculant in soil after recurrent inoculations
Abstract Microbial inoculants are attracting growing interest in agriculture, but their efficacy remains unreliable in relation to their poor survival, partly due to the competition with the soil resident community. We hypothesised that recurrent inoculation could gradually alleviate this competitio...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-02-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-54069-x |
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| author | M. Papin L. Philippot M. C. Breuil D. Bru A. Dreux-Zigha A. Mounier X. Le Roux N. Rouard A. Spor |
| author_facet | M. Papin L. Philippot M. C. Breuil D. Bru A. Dreux-Zigha A. Mounier X. Le Roux N. Rouard A. Spor |
| author_sort | M. Papin |
| collection | DOAJ |
| description | Abstract Microbial inoculants are attracting growing interest in agriculture, but their efficacy remains unreliable in relation to their poor survival, partly due to the competition with the soil resident community. We hypothesised that recurrent inoculation could gradually alleviate this competition and improve the survival of the inoculant while increasing its impact on the resident bacterial community. We tested the effectiveness of such strategy with four inoculation sequences of Pseudomonas fluorescens strain B177 in soil microcosms with increasing number and frequency of inoculation, compared to a non-inoculated control. Each sequence was carried out at two inoculation densities (106 and 108 cfu.g soil−1). The four-inoculation sequence induced a higher abundance of P. fluorescens, 2 weeks after the last inoculation. No impact of inoculation sequences was observed on the resident community diversity and composition. Differential abundance analysis identified only 28 out of 576 dominants OTUs affected by the high-density inoculum, whatever the inoculation sequence. Recurrent inoculations induced a strong accumulation of nitrate, not explained by the abundance of nitrifying or nitrate-reducing microorganisms. In summary, inoculant density rather than inoculation pattern matters for inoculation effect on the resident bacterial communities, while recurrent inoculation allowed to slightly enhance the survival of the inoculant and strongly increased soil nitrate content. |
| first_indexed | 2024-03-07T15:08:23Z |
| format | Article |
| id | doaj.art-9533547b7f5143e6bd0ce7e72e8bbe9b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:08:23Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9533547b7f5143e6bd0ce7e72e8bbe9b2024-03-05T18:49:02ZengNature PortfolioScientific Reports2045-23222024-02-0114111210.1038/s41598-024-54069-xSurvival of a microbial inoculant in soil after recurrent inoculationsM. Papin0L. Philippot1M. C. Breuil2D. Bru3A. Dreux-Zigha4A. Mounier5X. Le Roux6N. Rouard7A. Spor8Univ Bourgogne Franche Comte, INRAE, Institut Agro Dijon, AgroecologieUniv Bourgogne Franche Comte, INRAE, Institut Agro Dijon, AgroecologieUniv Bourgogne Franche Comte, INRAE, Institut Agro Dijon, AgroecologieUniv Bourgogne Franche Comte, INRAE, Institut Agro Dijon, AgroecologieGreenCell Biopole Clermont LimagneUniv Bourgogne Franche Comte, INRAE, Institut Agro Dijon, AgroecologieUniversite Claude Bernard Lyon 1, Microbial Ecology Centre LEM, INRAE, CNRS, VetAgroSup, UMR INRAE 1418Univ Bourgogne Franche Comte, INRAE, Institut Agro Dijon, AgroecologieUniv Bourgogne Franche Comte, INRAE, Institut Agro Dijon, AgroecologieAbstract Microbial inoculants are attracting growing interest in agriculture, but their efficacy remains unreliable in relation to their poor survival, partly due to the competition with the soil resident community. We hypothesised that recurrent inoculation could gradually alleviate this competition and improve the survival of the inoculant while increasing its impact on the resident bacterial community. We tested the effectiveness of such strategy with four inoculation sequences of Pseudomonas fluorescens strain B177 in soil microcosms with increasing number and frequency of inoculation, compared to a non-inoculated control. Each sequence was carried out at two inoculation densities (106 and 108 cfu.g soil−1). The four-inoculation sequence induced a higher abundance of P. fluorescens, 2 weeks after the last inoculation. No impact of inoculation sequences was observed on the resident community diversity and composition. Differential abundance analysis identified only 28 out of 576 dominants OTUs affected by the high-density inoculum, whatever the inoculation sequence. Recurrent inoculations induced a strong accumulation of nitrate, not explained by the abundance of nitrifying or nitrate-reducing microorganisms. In summary, inoculant density rather than inoculation pattern matters for inoculation effect on the resident bacterial communities, while recurrent inoculation allowed to slightly enhance the survival of the inoculant and strongly increased soil nitrate content.https://doi.org/10.1038/s41598-024-54069-xPGPRNitrogen cycleSoil bacteriaRecurrent inoculation |
| spellingShingle | M. Papin L. Philippot M. C. Breuil D. Bru A. Dreux-Zigha A. Mounier X. Le Roux N. Rouard A. Spor Survival of a microbial inoculant in soil after recurrent inoculations Scientific Reports PGPR Nitrogen cycle Soil bacteria Recurrent inoculation |
| title | Survival of a microbial inoculant in soil after recurrent inoculations |
| title_full | Survival of a microbial inoculant in soil after recurrent inoculations |
| title_fullStr | Survival of a microbial inoculant in soil after recurrent inoculations |
| title_full_unstemmed | Survival of a microbial inoculant in soil after recurrent inoculations |
| title_short | Survival of a microbial inoculant in soil after recurrent inoculations |
| title_sort | survival of a microbial inoculant in soil after recurrent inoculations |
| topic | PGPR Nitrogen cycle Soil bacteria Recurrent inoculation |
| url | https://doi.org/10.1038/s41598-024-54069-x |
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