Visible gland constantly traces virus-induced gene silencing in cotton
A virus-induced gene silencing (VIGS) system was established to induce endogenous target gene silencing by post-transcriptional gene silencing (PTGS), which is a powerful tool for gene function analysis in plants. Compared with stable transgenic plant via Agrobacterium-mediated gene transformation,...
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| Format: | Article |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1020841/full |
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| author | Zhanfeng Si Huaitong Wu Huaitong Wu Yue Tian Zhiyuan Zhang Tianzhen Zhang Tianzhen Zhang Yan Hu Yan Hu |
| author_facet | Zhanfeng Si Huaitong Wu Huaitong Wu Yue Tian Zhiyuan Zhang Tianzhen Zhang Tianzhen Zhang Yan Hu Yan Hu |
| author_sort | Zhanfeng Si |
| collection | DOAJ |
| description | A virus-induced gene silencing (VIGS) system was established to induce endogenous target gene silencing by post-transcriptional gene silencing (PTGS), which is a powerful tool for gene function analysis in plants. Compared with stable transgenic plant via Agrobacterium-mediated gene transformation, phenotypes after gene knockdown can be obtained rapidly, effectively, and high-throughput through VIGS system. This approach has been successfully applied to explore unknown gene functions involved in plant growth and development, physiological metabolism, and biotic and abiotic stresses in various plants. In this system, GhCLA1 was used as a general control, however, silencing of this gene leads to leaf albino, wilting, and plant death ultimately. As such, it cannot indicate the efficiency of target gene silencing throughout the whole plant growth period. To address this question, in this study, we developed a novel marker gene, Gossypium PIGMENT GLAND FORMATION GENE (GoPGF), as the control to trace the efficiency of gene silencing in the infected tissues. GoPGF has been proved a key gene in gland forming. Suppression of GoPGF does not affect the normal growth and development of cotton. The number of gland altered related to the expression level of GoPGF gene. So it is a good marker that be used to trace the whole growth stages of plant. Moreover, we further developed a method of friction inoculation to enhance and extend the efficiency of VIGS, which facilitates the analysis of gene function in both the vegetative stage and reproductive stage. This improved VIGS technology will be a powerful tool for the rapid functional identification of unknown genes in genomes. |
| first_indexed | 2024-04-12T18:53:01Z |
| format | Article |
| id | doaj.art-95344d0272b1422a9abd2dd3474fb460 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T18:53:01Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-95344d0272b1422a9abd2dd3474fb4602022-12-22T03:20:24ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.10208411020841Visible gland constantly traces virus-induced gene silencing in cottonZhanfeng Si0Huaitong Wu1Huaitong Wu2Yue Tian3Zhiyuan Zhang4Tianzhen Zhang5Tianzhen Zhang6Yan Hu7Yan Hu8Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, ChinaDepartment of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, ChinaKey Laboratory for Tree Breeding and Germplasm Improvement, Southern Modern Forestry Collaborative Innovation Center, College of Forestry, Nanjing Forestry University, Nanjing, ChinaCollege of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, ChinaHainan Institute of Zhejiang University, Sanya, ChinaDepartment of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, ChinaHainan Institute of Zhejiang University, Sanya, ChinaDepartment of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, ChinaHainan Institute of Zhejiang University, Sanya, ChinaA virus-induced gene silencing (VIGS) system was established to induce endogenous target gene silencing by post-transcriptional gene silencing (PTGS), which is a powerful tool for gene function analysis in plants. Compared with stable transgenic plant via Agrobacterium-mediated gene transformation, phenotypes after gene knockdown can be obtained rapidly, effectively, and high-throughput through VIGS system. This approach has been successfully applied to explore unknown gene functions involved in plant growth and development, physiological metabolism, and biotic and abiotic stresses in various plants. In this system, GhCLA1 was used as a general control, however, silencing of this gene leads to leaf albino, wilting, and plant death ultimately. As such, it cannot indicate the efficiency of target gene silencing throughout the whole plant growth period. To address this question, in this study, we developed a novel marker gene, Gossypium PIGMENT GLAND FORMATION GENE (GoPGF), as the control to trace the efficiency of gene silencing in the infected tissues. GoPGF has been proved a key gene in gland forming. Suppression of GoPGF does not affect the normal growth and development of cotton. The number of gland altered related to the expression level of GoPGF gene. So it is a good marker that be used to trace the whole growth stages of plant. Moreover, we further developed a method of friction inoculation to enhance and extend the efficiency of VIGS, which facilitates the analysis of gene function in both the vegetative stage and reproductive stage. This improved VIGS technology will be a powerful tool for the rapid functional identification of unknown genes in genomes.https://www.frontiersin.org/articles/10.3389/fpls.2022.1020841/fullcottongene function analysesVIGS (virus-induced gene silencing)marker genegland trait |
| spellingShingle | Zhanfeng Si Huaitong Wu Huaitong Wu Yue Tian Zhiyuan Zhang Tianzhen Zhang Tianzhen Zhang Yan Hu Yan Hu Visible gland constantly traces virus-induced gene silencing in cotton Frontiers in Plant Science cotton gene function analyses VIGS (virus-induced gene silencing) marker gene gland trait |
| title | Visible gland constantly traces virus-induced gene silencing in cotton |
| title_full | Visible gland constantly traces virus-induced gene silencing in cotton |
| title_fullStr | Visible gland constantly traces virus-induced gene silencing in cotton |
| title_full_unstemmed | Visible gland constantly traces virus-induced gene silencing in cotton |
| title_short | Visible gland constantly traces virus-induced gene silencing in cotton |
| title_sort | visible gland constantly traces virus induced gene silencing in cotton |
| topic | cotton gene function analyses VIGS (virus-induced gene silencing) marker gene gland trait |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1020841/full |
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