| Summary: | Cucumber, Cucumis sativus is one of the most cultivated vegetables in the world, which is mostly consumed raw in China. The complete chloroplast genome of C. sativus was assembled and annotated in this study. Its size was 155,525 bp, containing a large single-copy region of 86,878 bp, a small single-copy region of 18,269 bp and a pair of IR regions of 25,189 bp. Whole chloroplast genome of C. sativus contains 131 genes, including 77 protein-coding genes (PCGs), 46 transfer RNA genes (tRNAs), and 8 ribosome RNA genes (rRNAs). The overall nucleotide composition is: A of 31.1%, T of 31.9%, C of 18.8% and G of 18.2%, with a total GC content of the chloroplast genome 37.0% and AT of 63.0%. Genetic and phylogenetic analysis based on 10 plants species confirmed the position of C. sativus closely related to Cucurbitaceae species of Cucumis hystrix.
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