Determination and Comparison of Fluconazole Susceptibility by Two Different Microdilution Methods in Candida Strains Isolated from Hospitalized Immunocompromised Patients
Introduction: Clinical and Laboratory Standards (CLSI) Institute recommends CLSI M27-A method as a standard antifungal susceptibility test for Candida strains. The aim of this study was to compare United Kingdom Reference method with CLSI M27-A method. Materials and Methods: Seventy-five Candida spe...
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| Format: | Article |
| Language: | English |
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Bilimsel Tip Yayinevi
2008-09-01
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| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| Subjects: | |
| Online Access: | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2008-13-3-130-135.pdf |
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| author | Ecegül ALBAY BENGİ İlhan AFŞAR Koray CANDÜZ Nurdan BİLGİN Nükhet KURULTAY Metin TÜRKER |
| author_facet | Ecegül ALBAY BENGİ İlhan AFŞAR Koray CANDÜZ Nurdan BİLGİN Nükhet KURULTAY Metin TÜRKER |
| author_sort | Ecegül ALBAY BENGİ |
| collection | DOAJ |
| description | Introduction: Clinical and Laboratory Standards (CLSI) Institute recommends CLSI M27-A method as a standard antifungal susceptibility test for Candida strains. The aim of this study was to compare United Kingdom Reference method with CLSI M27-A method. Materials and Methods: Seventy-five Candida species isolated from various clinical samples from the hospitalized, immunocompromised patients were included in the study. Of the 75 Candida strains isolated, 61 (81.3%) were identified as Candida albicans, 6 (8%) as Candida glabrata, 3 (4%) as Candida parapsilosis, 3 (4%) as Candida guilliermondii and 2 (2.7%) as Candida tropicalis. The susceptibility of the identified species to fluconazole was evaluated by two different microdilution methods and the results were compared. For the standard microdilution method (CLSI M27-A) RPMI 1640 medium and for the alternative method (United Kingdom Reference method) Yeast Nitrogen Base Broth were used. Results: When ± 2 difference in minimal inhibitory concentration (MIC) values was used for evalution of agreement between the two media, the percent agreement rate was found as 84%. When ± 1 difference in MIC values was used for evalution of agreement between the two media, the percent agreement rate was found as 68%. Conclusion: United Kingdom Reference method seems as a good alternative testing of antifungal susceptibility for Candida strains. |
| first_indexed | 2024-04-10T11:21:24Z |
| format | Article |
| id | doaj.art-95363f4964e4419396c795dc3f018ec1 |
| institution | Directory Open Access Journal |
| issn | 1300-932X 1300-932X |
| language | English |
| last_indexed | 2024-04-10T11:21:24Z |
| publishDate | 2008-09-01 |
| publisher | Bilimsel Tip Yayinevi |
| record_format | Article |
| series | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| spelling | doaj.art-95363f4964e4419396c795dc3f018ec12023-02-15T16:18:39ZengBilimsel Tip YayineviFlora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi1300-932X1300-932X2008-09-01133130135Determination and Comparison of Fluconazole Susceptibility by Two Different Microdilution Methods in Candida Strains Isolated from Hospitalized Immunocompromised PatientsEcegül ALBAY BENGİ0İlhan AFŞAR1Koray CANDÜZ2Nurdan BİLGİN3Nükhet KURULTAY4Metin TÜRKER5Atatürk Eğitim ve Araştırma Hastanesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Laboratuvarı, İZMİRAtatürk Eğitim ve Araştırma Hastanesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Laboratuvarı, İZMİRAtatürk Eğitim ve Araştırma Hastanesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Laboratuvarı, İZMİRAtatürk Eğitim ve Araştırma Hastanesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Laboratuvarı, İZMİRAtatürk Eğitim ve Araştırma Hastanesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Laboratuvarı, İZMİRAtatürk Eğitim ve Araştırma Hastanesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Laboratuvarı, İZMİRIntroduction: Clinical and Laboratory Standards (CLSI) Institute recommends CLSI M27-A method as a standard antifungal susceptibility test for Candida strains. The aim of this study was to compare United Kingdom Reference method with CLSI M27-A method. Materials and Methods: Seventy-five Candida species isolated from various clinical samples from the hospitalized, immunocompromised patients were included in the study. Of the 75 Candida strains isolated, 61 (81.3%) were identified as Candida albicans, 6 (8%) as Candida glabrata, 3 (4%) as Candida parapsilosis, 3 (4%) as Candida guilliermondii and 2 (2.7%) as Candida tropicalis. The susceptibility of the identified species to fluconazole was evaluated by two different microdilution methods and the results were compared. For the standard microdilution method (CLSI M27-A) RPMI 1640 medium and for the alternative method (United Kingdom Reference method) Yeast Nitrogen Base Broth were used. Results: When ± 2 difference in minimal inhibitory concentration (MIC) values was used for evalution of agreement between the two media, the percent agreement rate was found as 84%. When ± 1 difference in MIC values was used for evalution of agreement between the two media, the percent agreement rate was found as 68%. Conclusion: United Kingdom Reference method seems as a good alternative testing of antifungal susceptibility for Candida strains.http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2008-13-3-130-135.pdfCandidaFluconazoleAntifungal susceptibility testingMicrodilutionRPMI 1640Yeast nitrogen base broth |
| spellingShingle | Ecegül ALBAY BENGİ İlhan AFŞAR Koray CANDÜZ Nurdan BİLGİN Nükhet KURULTAY Metin TÜRKER Determination and Comparison of Fluconazole Susceptibility by Two Different Microdilution Methods in Candida Strains Isolated from Hospitalized Immunocompromised Patients Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi Candida Fluconazole Antifungal susceptibility testing Microdilution RPMI 1640 Yeast nitrogen base broth |
| title | Determination and Comparison of Fluconazole Susceptibility by Two Different Microdilution Methods in Candida Strains Isolated from Hospitalized Immunocompromised Patients |
| title_full | Determination and Comparison of Fluconazole Susceptibility by Two Different Microdilution Methods in Candida Strains Isolated from Hospitalized Immunocompromised Patients |
| title_fullStr | Determination and Comparison of Fluconazole Susceptibility by Two Different Microdilution Methods in Candida Strains Isolated from Hospitalized Immunocompromised Patients |
| title_full_unstemmed | Determination and Comparison of Fluconazole Susceptibility by Two Different Microdilution Methods in Candida Strains Isolated from Hospitalized Immunocompromised Patients |
| title_short | Determination and Comparison of Fluconazole Susceptibility by Two Different Microdilution Methods in Candida Strains Isolated from Hospitalized Immunocompromised Patients |
| title_sort | determination and comparison of fluconazole susceptibility by two different microdilution methods in candida strains isolated from hospitalized immunocompromised patients |
| topic | Candida Fluconazole Antifungal susceptibility testing Microdilution RPMI 1640 Yeast nitrogen base broth |
| url | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2008-13-3-130-135.pdf |
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