Determination of Mucoadhesion of Polyvinyl Alcohol Films to Human Intestinal Tissue
The absorption of drugs with narrow absorption windows in the upper small intestine can be improved with a mucoadhesive drug delivery system such as enteric films. To predict the mucoadhesive behaviour in vivo, suitable in vitro or ex vivo methods can be performed. In this study, the influence of ti...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/6/1740 |
| _version_ | 1797593060105781248 |
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| author | Laura Müller Christoph Rosenbaum Adrian Rump Michael Grimm Friederike Klammt Annabel Kleinwort Alexandra Busemann Werner Weitschies |
| author_facet | Laura Müller Christoph Rosenbaum Adrian Rump Michael Grimm Friederike Klammt Annabel Kleinwort Alexandra Busemann Werner Weitschies |
| author_sort | Laura Müller |
| collection | DOAJ |
| description | The absorption of drugs with narrow absorption windows in the upper small intestine can be improved with a mucoadhesive drug delivery system such as enteric films. To predict the mucoadhesive behaviour in vivo, suitable in vitro or ex vivo methods can be performed. In this study, the influence of tissue storage and sampling site on the mucoadhesion of polyvinyl alcohol film to human small intestinal mucosa was investigated. Tissue from twelve human subjects was used to determine adhesion using a tensile strength method. Thawing of tissue frozen at −20 °C resulted in a significantly higher work of adhesion (<i>p</i> = 0.0005) when a low contact force was applied for one minute, whereas the maximum detachment force was not affected. When the contact force and time were increased, no differences were found for thawed tissue compared to fresh tissue. No change in adhesion was observed depending on the sampling location. Initial results from a comparison of adhesion to porcine and human mucosa suggest that the tissues are equivalent. |
| first_indexed | 2024-03-11T02:02:14Z |
| format | Article |
| id | doaj.art-953642b8ece042f8843b5050ccc36363 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-11T02:02:14Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-953642b8ece042f8843b5050ccc363632023-11-18T12:05:54ZengMDPI AGPharmaceutics1999-49232023-06-01156174010.3390/pharmaceutics15061740Determination of Mucoadhesion of Polyvinyl Alcohol Films to Human Intestinal TissueLaura Müller0Christoph Rosenbaum1Adrian Rump2Michael Grimm3Friederike Klammt4Annabel Kleinwort5Alexandra Busemann6Werner Weitschies7Department of Biopharmaceutics and Pharmaceutical Technology, Institute of Pharmacy, University of Greifswald, Felix-Hausdorff-Str. 3, 17487 Greifswald, GermanyDepartment of Biopharmaceutics and Pharmaceutical Technology, Institute of Pharmacy, University of Greifswald, Felix-Hausdorff-Str. 3, 17487 Greifswald, GermanyDepartment of Biopharmaceutics and Pharmaceutical Technology, Institute of Pharmacy, University of Greifswald, Felix-Hausdorff-Str. 3, 17487 Greifswald, GermanyDepartment of Biopharmaceutics and Pharmaceutical Technology, Institute of Pharmacy, University of Greifswald, Felix-Hausdorff-Str. 3, 17487 Greifswald, GermanyDepartment of Biopharmaceutics and Pharmaceutical Technology, Institute of Pharmacy, University of Greifswald, Felix-Hausdorff-Str. 3, 17487 Greifswald, GermanyDepartment of General, Visceral, Thoracic and Vascular Surgery, Greifswald University Medicine, Ferdinand-Sauerbruch-Str., 17457 Greifswald, GermanyDepartment of General, Visceral, Thoracic and Vascular Surgery, Greifswald University Medicine, Ferdinand-Sauerbruch-Str., 17457 Greifswald, GermanyDepartment of Biopharmaceutics and Pharmaceutical Technology, Institute of Pharmacy, University of Greifswald, Felix-Hausdorff-Str. 3, 17487 Greifswald, GermanyThe absorption of drugs with narrow absorption windows in the upper small intestine can be improved with a mucoadhesive drug delivery system such as enteric films. To predict the mucoadhesive behaviour in vivo, suitable in vitro or ex vivo methods can be performed. In this study, the influence of tissue storage and sampling site on the mucoadhesion of polyvinyl alcohol film to human small intestinal mucosa was investigated. Tissue from twelve human subjects was used to determine adhesion using a tensile strength method. Thawing of tissue frozen at −20 °C resulted in a significantly higher work of adhesion (<i>p</i> = 0.0005) when a low contact force was applied for one minute, whereas the maximum detachment force was not affected. When the contact force and time were increased, no differences were found for thawed tissue compared to fresh tissue. No change in adhesion was observed depending on the sampling location. Initial results from a comparison of adhesion to porcine and human mucosa suggest that the tissues are equivalent.https://www.mdpi.com/1999-4923/15/6/1740mucoadhesionsite-specific applicationintestinal applicationex vivo measurementshuman intestinal mucosa |
| spellingShingle | Laura Müller Christoph Rosenbaum Adrian Rump Michael Grimm Friederike Klammt Annabel Kleinwort Alexandra Busemann Werner Weitschies Determination of Mucoadhesion of Polyvinyl Alcohol Films to Human Intestinal Tissue Pharmaceutics mucoadhesion site-specific application intestinal application ex vivo measurements human intestinal mucosa |
| title | Determination of Mucoadhesion of Polyvinyl Alcohol Films to Human Intestinal Tissue |
| title_full | Determination of Mucoadhesion of Polyvinyl Alcohol Films to Human Intestinal Tissue |
| title_fullStr | Determination of Mucoadhesion of Polyvinyl Alcohol Films to Human Intestinal Tissue |
| title_full_unstemmed | Determination of Mucoadhesion of Polyvinyl Alcohol Films to Human Intestinal Tissue |
| title_short | Determination of Mucoadhesion of Polyvinyl Alcohol Films to Human Intestinal Tissue |
| title_sort | determination of mucoadhesion of polyvinyl alcohol films to human intestinal tissue |
| topic | mucoadhesion site-specific application intestinal application ex vivo measurements human intestinal mucosa |
| url | https://www.mdpi.com/1999-4923/15/6/1740 |
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