Key genes and pathways involved in the pathogenesis of pemphigus foliaceus: Based on bioinformatic studies
Objective Using bioinformatic technique to identify key genes and pathways involved in the pathogenesis of pemphigus foliaceus (PF), in order to elucidate the pathogenesis of PF. Methods Gene microarray dataset GSE53873 of peripheral blood CD4+ T cells from PF patients was obtained from Gene Express...
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| Format: | Article |
| Language: | zho |
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editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology
2023-04-01
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| Series: | Pifu-xingbing zhenliaoxue zazhi |
| Subjects: | |
| Online Access: | http://pfxbzlx.gdvdc.com/EN/10.3969/j.issn.1674-8468.2023.02.002 |
| _version_ | 1827396942205812736 |
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| author | Yaqian ZHU Xinhui LI Ziyue JIA Yongfeng CHEN |
| author_facet | Yaqian ZHU Xinhui LI Ziyue JIA Yongfeng CHEN |
| author_sort | Yaqian ZHU |
| collection | DOAJ |
| description | Objective Using bioinformatic technique to identify key genes and pathways involved in the pathogenesis of pemphigus foliaceus (PF), in order to elucidate the pathogenesis of PF. Methods Gene microarray dataset GSE53873 of peripheral blood CD4+ T cells from PF patients was obtained from Gene Expression Omnibus database, and various bioinformatics methods such as Limma difference analysis, gene function and pathway enrichment analysis, and protein-protein interaction network analysis were integrated to determine the key genes and pathways associated with PF. Results A total of 121 significantly upregulated genes and 48 significantly downregulated genes were identified in PF patients compared with healthy controls. The differentially expressed genes in PF patients were involved in multiple functions such as T cell differentiation, T cell activation, leukocyte differentiation and metabolism. CXCL10 and OAS1 were identified as key genes in the pathogenesis of PF. Receiver operating characteristic (ROC) results suggested that CXCL10 (AUC=0.86) and OAS1 (AUC=0.86) had high diagnostic efficacy for PF. Gene function and pathway enrichment analysis indicated that CXCL10 was mainly associated with TOLL-like receptor signaling pathway, COVID-19 infection, and cytokine interaction, while OSA1 was mainly associated with viral infection and type I interferon signaling pathway. Conclusions CXCL10 and OAS1 are the key genes associated with PF. The pathogenesis of pemphigus is associated with pathways such as T cell differentiation and activation, leukocyte differentiation and metabolism. |
| first_indexed | 2024-03-08T19:00:00Z |
| format | Article |
| id | doaj.art-95395a33efcf47a48cc9eebdb17f40c5 |
| institution | Directory Open Access Journal |
| issn | 1674-8468 |
| language | zho |
| last_indexed | 2024-03-08T19:00:00Z |
| publishDate | 2023-04-01 |
| publisher | editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology |
| record_format | Article |
| series | Pifu-xingbing zhenliaoxue zazhi |
| spelling | doaj.art-95395a33efcf47a48cc9eebdb17f40c52023-12-28T06:52:55Zzhoeditoiral office of Journal of Diagnosis and Therapy on Dermato-venereologyPifu-xingbing zhenliaoxue zazhi1674-84682023-04-0130210010610.3969/j.issn.1674-8468.2023.02.002Key genes and pathways involved in the pathogenesis of pemphigus foliaceus: Based on bioinformatic studiesYaqian ZHU0Xinhui LI1Ziyue JIA2Yongfeng CHEN3Guangdong Medical College, College of Dermatology, Anhui Medical University, Guangzhou 510091, ChinaDermatology Hospital, Southern Medical University, Guangzhou 510091, ChinaDermatology Hospital, Southern Medical University, Guangzhou 510091, ChinaGuangdong Medical College, College of Dermatology, Anhui Medical University, Guangzhou 510091, ChinaObjective Using bioinformatic technique to identify key genes and pathways involved in the pathogenesis of pemphigus foliaceus (PF), in order to elucidate the pathogenesis of PF. Methods Gene microarray dataset GSE53873 of peripheral blood CD4+ T cells from PF patients was obtained from Gene Expression Omnibus database, and various bioinformatics methods such as Limma difference analysis, gene function and pathway enrichment analysis, and protein-protein interaction network analysis were integrated to determine the key genes and pathways associated with PF. Results A total of 121 significantly upregulated genes and 48 significantly downregulated genes were identified in PF patients compared with healthy controls. The differentially expressed genes in PF patients were involved in multiple functions such as T cell differentiation, T cell activation, leukocyte differentiation and metabolism. CXCL10 and OAS1 were identified as key genes in the pathogenesis of PF. Receiver operating characteristic (ROC) results suggested that CXCL10 (AUC=0.86) and OAS1 (AUC=0.86) had high diagnostic efficacy for PF. Gene function and pathway enrichment analysis indicated that CXCL10 was mainly associated with TOLL-like receptor signaling pathway, COVID-19 infection, and cytokine interaction, while OSA1 was mainly associated with viral infection and type I interferon signaling pathway. Conclusions CXCL10 and OAS1 are the key genes associated with PF. The pathogenesis of pemphigus is associated with pathways such as T cell differentiation and activation, leukocyte differentiation and metabolism.http://pfxbzlx.gdvdc.com/EN/10.3969/j.issn.1674-8468.2023.02.002pemphigus foliaceusgenome sequencingcxcl10oas1cd4+ t cell |
| spellingShingle | Yaqian ZHU Xinhui LI Ziyue JIA Yongfeng CHEN Key genes and pathways involved in the pathogenesis of pemphigus foliaceus: Based on bioinformatic studies Pifu-xingbing zhenliaoxue zazhi pemphigus foliaceus genome sequencing cxcl10 oas1 cd4+ t cell |
| title | Key genes and pathways involved in the pathogenesis of pemphigus foliaceus: Based on bioinformatic studies |
| title_full | Key genes and pathways involved in the pathogenesis of pemphigus foliaceus: Based on bioinformatic studies |
| title_fullStr | Key genes and pathways involved in the pathogenesis of pemphigus foliaceus: Based on bioinformatic studies |
| title_full_unstemmed | Key genes and pathways involved in the pathogenesis of pemphigus foliaceus: Based on bioinformatic studies |
| title_short | Key genes and pathways involved in the pathogenesis of pemphigus foliaceus: Based on bioinformatic studies |
| title_sort | key genes and pathways involved in the pathogenesis of pemphigus foliaceus based on bioinformatic studies |
| topic | pemphigus foliaceus genome sequencing cxcl10 oas1 cd4+ t cell |
| url | http://pfxbzlx.gdvdc.com/EN/10.3969/j.issn.1674-8468.2023.02.002 |
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