Sequence analysis and characterization of rolling-circle replicating plasmid pVCM01 from Salmonella enterica
Aims: Characterization of cryptic plasmid pVCM01 (accession number JX133088) isolated from Salmonella enterica Enteritidis. Methodology and results: The complete sequence of pVCM01 was obtained. This plasmid possesses 1981 bp, with G+C content of 57% in agreement of the range of Salmonella genomic...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Malaysian Society for Microbiology
2013-12-01
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| Series: | Malaysian Journal of Microbiology |
| Subjects: | |
| Online Access: | http://web.usm.my/mjm/issues/vol9no4/Short%202.pdf |
| Summary: | Aims: Characterization of cryptic plasmid pVCM01 (accession number JX133088) isolated from Salmonella enterica Enteritidis.
Methodology and results: The complete sequence of pVCM01 was obtained. This plasmid possesses 1981 bp, with G+C content of 57% in agreement of the range of Salmonella genomic DNA. pVCM01 has a high degree of similarity to pB and pJ plasmids. It possesses six main open reading frames, only one have a very high degree of amino acid identity with protein involved in the rolling-circle-like replication (RCR). Based on the sequence similarities, pVCM01 plasmid belonged to the pC194/pUB110 rolling-circle replicating plasmid family. The Rep pVCM01 possesses the motifs: FLTLTVRN, HPHFHTL, SGDGYVKHERW, which were present in all Rep proteins.
Conclusion, significance and impact of study: The small size of pVCM01 plasmid and its stability in E. coli cells, make it an attractive candidate to develop new vectors, such as cloning and/or expression vector. |
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| ISSN: | 1823-8262 2231-7538 |