Optical detection of three modes of endocytosis at hippocampal synapses
Coupling of synaptic vesicle fusion and retrieval constitutes a core mechanism ensuring maintenance of presynaptic function. Recent studies using fast-freeze electron microscopy and capacitance measurements reported an ultrafast mode of endocytosis operating at physiological temperatures. Here, usin...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2018-04-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/36097 |
| _version_ | 1818019839304794112 |
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| author | Natali L Chanaday Ege T Kavalali |
| author_facet | Natali L Chanaday Ege T Kavalali |
| author_sort | Natali L Chanaday |
| collection | DOAJ |
| description | Coupling of synaptic vesicle fusion and retrieval constitutes a core mechanism ensuring maintenance of presynaptic function. Recent studies using fast-freeze electron microscopy and capacitance measurements reported an ultrafast mode of endocytosis operating at physiological temperatures. Here, using rat hippocampal neurons, we optically monitored single synaptic vesicle endocytosis with high time resolution using the vesicular glutamate transporter, synaptophysin and the V0a1 subunit of the vacuolar ATPase as probes. In this setting, we could distinguish three components of retrieval operating at ultrafast (~150–250 ms, ~20% of events), fast (~5–12 s, ~40% of events) and ultraslow speeds (>20 s, ~40% of events). While increasing Ca2+ slowed the fast events, increasing temperature accelerated their time course. In contrast, the kinetics of ultrafast events were only mildly affected by these manipulations. These results suggest that synaptic vesicle proteins can be retrieved with ultrafast kinetics, although a majority of evoked fusion events are coupled to slower retrieval mechanisms. |
| first_indexed | 2024-04-14T07:57:44Z |
| format | Article |
| id | doaj.art-953a3e72915b4a76bd5e0ae6eb587536 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-14T07:57:44Z |
| publishDate | 2018-04-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-953a3e72915b4a76bd5e0ae6eb5875362022-12-22T02:05:00ZengeLife Sciences Publications LtdeLife2050-084X2018-04-01710.7554/eLife.36097Optical detection of three modes of endocytosis at hippocampal synapsesNatali L Chanaday0https://orcid.org/0000-0002-3376-5187Ege T Kavalali1https://orcid.org/0000-0003-1777-227XDepartment of Neuroscience, University of Texas Southwestern Medical Center, Dallas, United StatesDepartment of Neuroscience, University of Texas Southwestern Medical Center, Dallas, United StatesCoupling of synaptic vesicle fusion and retrieval constitutes a core mechanism ensuring maintenance of presynaptic function. Recent studies using fast-freeze electron microscopy and capacitance measurements reported an ultrafast mode of endocytosis operating at physiological temperatures. Here, using rat hippocampal neurons, we optically monitored single synaptic vesicle endocytosis with high time resolution using the vesicular glutamate transporter, synaptophysin and the V0a1 subunit of the vacuolar ATPase as probes. In this setting, we could distinguish three components of retrieval operating at ultrafast (~150–250 ms, ~20% of events), fast (~5–12 s, ~40% of events) and ultraslow speeds (>20 s, ~40% of events). While increasing Ca2+ slowed the fast events, increasing temperature accelerated their time course. In contrast, the kinetics of ultrafast events were only mildly affected by these manipulations. These results suggest that synaptic vesicle proteins can be retrieved with ultrafast kinetics, although a majority of evoked fusion events are coupled to slower retrieval mechanisms.https://elifesciences.org/articles/36097synaptic vesicle recyclingsynaptic terminalstemperatureendocytosissingle vesicle releaseneuroscience |
| spellingShingle | Natali L Chanaday Ege T Kavalali Optical detection of three modes of endocytosis at hippocampal synapses eLife synaptic vesicle recycling synaptic terminals temperature endocytosis single vesicle release neuroscience |
| title | Optical detection of three modes of endocytosis at hippocampal synapses |
| title_full | Optical detection of three modes of endocytosis at hippocampal synapses |
| title_fullStr | Optical detection of three modes of endocytosis at hippocampal synapses |
| title_full_unstemmed | Optical detection of three modes of endocytosis at hippocampal synapses |
| title_short | Optical detection of three modes of endocytosis at hippocampal synapses |
| title_sort | optical detection of three modes of endocytosis at hippocampal synapses |
| topic | synaptic vesicle recycling synaptic terminals temperature endocytosis single vesicle release neuroscience |
| url | https://elifesciences.org/articles/36097 |
| work_keys_str_mv | AT natalilchanaday opticaldetectionofthreemodesofendocytosisathippocampalsynapses AT egetkavalali opticaldetectionofthreemodesofendocytosisathippocampalsynapses |