Immediate to short-term inflammatory response to biomaterial implanted in calvarium of mice
Scaffolds made of biodegradable materials play a very important role in repairing bone defects. Our study was conducted with the aim of investigating inflammation, vascular changes, and tissue necrosis after the placement of 3D printed scaffolds composed of beta-tricalcium phosphate (TCP-β) on the...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2022-09-01
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| Series: | European Journal of Translational Myology |
| Subjects: | |
| Online Access: | https://www.pagepressjournals.org/index.php/bam/article/view/10785 |
| _version_ | 1818059087941730304 |
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| author | Saba Akbari Eshagh Ali Saberi Sirous Risbaf Fakour Zahra Heidari |
| author_facet | Saba Akbari Eshagh Ali Saberi Sirous Risbaf Fakour Zahra Heidari |
| author_sort | Saba Akbari |
| collection | DOAJ |
| description |
Scaffolds made of biodegradable materials play a very important role in repairing bone defects. Our study was conducted with the aim of investigating inflammation, vascular changes, and tissue necrosis after the placement of 3D printed scaffolds composed of beta-tricalcium phosphate (TCP-β) on the calvarial bone defect of mice. Eight samples of scalp tissue in mice were examined in four groups (one-week control, two-week control, one-week experiment, and two-week experiment). Mice with routine bone defects were selected as the control group and mice with bone defects with β-TCP scaffolds were selected as the experimental group (TCP). The groups were evaluated in terms of inflammatory cells, osteoblast and osteoclast cells, vascular changes, and the number of resorption pit and empty lacuna. The results demonstrated a decrease in inflammatory cells and an increase in osteoclast and osteoblast cells in bone defect sites placed with TCP-β scaffolds (p<0.05). The results of histological staining showed pit resorption and further vascularization in the place of TCP-β scaffolds, but these changes were not statistically significant (p>0.05). Examining the number of empty lacunae in the bone defect site showed that TCP-β could significantly reduce the number of these lacunae in the bone defect sites placed with TCP-β scaffolds (p<0.05). 3D printed scaffolds composed of TCP-β that were implanted in bone defect sites were effective in reducing the inflammatory responses, emptying lacunae and increasing bone regeneration.
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| first_indexed | 2024-12-10T13:10:57Z |
| format | Article |
| id | doaj.art-953ac3b500064b018f55254b501ad7c3 |
| institution | Directory Open Access Journal |
| issn | 2037-7452 2037-7460 |
| language | English |
| last_indexed | 2024-12-10T13:10:57Z |
| publishDate | 2022-09-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Translational Myology |
| spelling | doaj.art-953ac3b500064b018f55254b501ad7c32022-12-22T01:47:41ZengPAGEPress PublicationsEuropean Journal of Translational Myology2037-74522037-74602022-09-0110.4081/ejtm.2022.10785Immediate to short-term inflammatory response to biomaterial implanted in calvarium of miceSaba Akbari0Eshagh Ali Saberi1Sirous Risbaf Fakour2Zahra Heidari3Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Zahedan University of Medical Science, ZahedanOral and Dental Diseases Research Center, Department of Endodontics, Faculty of Dentistry, Zahedan University of Medical Sciences, ZahedanDepartment of Oral and Maxillofacial Surgery, Zahedan University of Medical Sciences, ZahedanDepartment of Histology, School of Medical Sciences, Zahedan University of Medical Sciences, Zahedan Scaffolds made of biodegradable materials play a very important role in repairing bone defects. Our study was conducted with the aim of investigating inflammation, vascular changes, and tissue necrosis after the placement of 3D printed scaffolds composed of beta-tricalcium phosphate (TCP-β) on the calvarial bone defect of mice. Eight samples of scalp tissue in mice were examined in four groups (one-week control, two-week control, one-week experiment, and two-week experiment). Mice with routine bone defects were selected as the control group and mice with bone defects with β-TCP scaffolds were selected as the experimental group (TCP). The groups were evaluated in terms of inflammatory cells, osteoblast and osteoclast cells, vascular changes, and the number of resorption pit and empty lacuna. The results demonstrated a decrease in inflammatory cells and an increase in osteoclast and osteoblast cells in bone defect sites placed with TCP-β scaffolds (p<0.05). The results of histological staining showed pit resorption and further vascularization in the place of TCP-β scaffolds, but these changes were not statistically significant (p>0.05). Examining the number of empty lacunae in the bone defect site showed that TCP-β could significantly reduce the number of these lacunae in the bone defect sites placed with TCP-β scaffolds (p<0.05). 3D printed scaffolds composed of TCP-β that were implanted in bone defect sites were effective in reducing the inflammatory responses, emptying lacunae and increasing bone regeneration. https://www.pagepressjournals.org/index.php/bam/article/view/10785Inflammationvascular changestissue necrosisbeta-tricalcium phosphatebone defectcalvarium |
| spellingShingle | Saba Akbari Eshagh Ali Saberi Sirous Risbaf Fakour Zahra Heidari Immediate to short-term inflammatory response to biomaterial implanted in calvarium of mice European Journal of Translational Myology Inflammation vascular changes tissue necrosis beta-tricalcium phosphate bone defect calvarium |
| title | Immediate to short-term inflammatory response to biomaterial implanted in calvarium of mice |
| title_full | Immediate to short-term inflammatory response to biomaterial implanted in calvarium of mice |
| title_fullStr | Immediate to short-term inflammatory response to biomaterial implanted in calvarium of mice |
| title_full_unstemmed | Immediate to short-term inflammatory response to biomaterial implanted in calvarium of mice |
| title_short | Immediate to short-term inflammatory response to biomaterial implanted in calvarium of mice |
| title_sort | immediate to short term inflammatory response to biomaterial implanted in calvarium of mice |
| topic | Inflammation vascular changes tissue necrosis beta-tricalcium phosphate bone defect calvarium |
| url | https://www.pagepressjournals.org/index.php/bam/article/view/10785 |
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