Fermentative α-Humulene Production from Homogenized Grass Clippings as a Growth Medium
Green waste, e.g., grass clippings, is currently insufficiently recycled and has untapped potential as a valuable resource. Our aim was to use juice from grass clippings as a growth medium for microorganisms. Herein, we demonstrate the production of the sesquiterpene α-humulene with the versatile or...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/24/8684 |
| _version_ | 1797456092528115712 |
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| author | Alexander Langsdorf Anna-Lena Drommershausen Marianne Volkmar Roland Ulber Dirk Holtmann |
| author_facet | Alexander Langsdorf Anna-Lena Drommershausen Marianne Volkmar Roland Ulber Dirk Holtmann |
| author_sort | Alexander Langsdorf |
| collection | DOAJ |
| description | Green waste, e.g., grass clippings, is currently insufficiently recycled and has untapped potential as a valuable resource. Our aim was to use juice from grass clippings as a growth medium for microorganisms. Herein, we demonstrate the production of the sesquiterpene α-humulene with the versatile organism <i>Cupriavidus necator</i> pKR-hum on a growth medium from grass clippings. The medium was compared with established media in terms of microbial growth and terpene production. <i>C. necator</i> pKR-hum shows a maximum growth rate of 0.43 h<sup>−1</sup> in the grass medium and 0.50 h<sup>−1</sup> in a lysogeny broth (LB) medium. With the grass medium, 2 mg/L of α-humulene were produced compared to 10 mg/L with the LB medium. By concentrating the grass medium and using a controlled bioreactor in combination with an optimized in situ product removal, comparable product concentrations could likely be achieved. To the best of our knowledge, this is the first time that juice from grass clippings has been used as a growth medium without any further additives for microbial product synthesis. This use of green waste as a material represents a new bioeconomic utilization option of waste materials and could contribute to improving the economics of grass biorefineries. |
| first_indexed | 2024-03-09T16:03:26Z |
| format | Article |
| id | doaj.art-953b13c33ca84079bd303592a335ae04 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T16:03:26Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-953b13c33ca84079bd303592a335ae042023-11-24T16:55:38ZengMDPI AGMolecules1420-30492022-12-012724868410.3390/molecules27248684Fermentative α-Humulene Production from Homogenized Grass Clippings as a Growth MediumAlexander Langsdorf0Anna-Lena Drommershausen1Marianne Volkmar2Roland Ulber3Dirk Holtmann4Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Wiesenstrasse 14, D-35390 Giessen, GermanyInstitute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Wiesenstrasse 14, D-35390 Giessen, GermanyInstitute of Bioprocess Engineering, University of Kaiserslautern, Gottlieb-Daimler-Strasse 49, D-67663 Kaiserslautern, GermanyInstitute of Bioprocess Engineering, University of Kaiserslautern, Gottlieb-Daimler-Strasse 49, D-67663 Kaiserslautern, GermanyInstitute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Wiesenstrasse 14, D-35390 Giessen, GermanyGreen waste, e.g., grass clippings, is currently insufficiently recycled and has untapped potential as a valuable resource. Our aim was to use juice from grass clippings as a growth medium for microorganisms. Herein, we demonstrate the production of the sesquiterpene α-humulene with the versatile organism <i>Cupriavidus necator</i> pKR-hum on a growth medium from grass clippings. The medium was compared with established media in terms of microbial growth and terpene production. <i>C. necator</i> pKR-hum shows a maximum growth rate of 0.43 h<sup>−1</sup> in the grass medium and 0.50 h<sup>−1</sup> in a lysogeny broth (LB) medium. With the grass medium, 2 mg/L of α-humulene were produced compared to 10 mg/L with the LB medium. By concentrating the grass medium and using a controlled bioreactor in combination with an optimized in situ product removal, comparable product concentrations could likely be achieved. To the best of our knowledge, this is the first time that juice from grass clippings has been used as a growth medium without any further additives for microbial product synthesis. This use of green waste as a material represents a new bioeconomic utilization option of waste materials and could contribute to improving the economics of grass biorefineries.https://www.mdpi.com/1420-3049/27/24/8684green waste<i>Cupriavidus necator</i>α-humuleneterpenoidbiorefinerybioeconomy |
| spellingShingle | Alexander Langsdorf Anna-Lena Drommershausen Marianne Volkmar Roland Ulber Dirk Holtmann Fermentative α-Humulene Production from Homogenized Grass Clippings as a Growth Medium Molecules green waste <i>Cupriavidus necator</i> α-humulene terpenoid biorefinery bioeconomy |
| title | Fermentative α-Humulene Production from Homogenized Grass Clippings as a Growth Medium |
| title_full | Fermentative α-Humulene Production from Homogenized Grass Clippings as a Growth Medium |
| title_fullStr | Fermentative α-Humulene Production from Homogenized Grass Clippings as a Growth Medium |
| title_full_unstemmed | Fermentative α-Humulene Production from Homogenized Grass Clippings as a Growth Medium |
| title_short | Fermentative α-Humulene Production from Homogenized Grass Clippings as a Growth Medium |
| title_sort | fermentative α humulene production from homogenized grass clippings as a growth medium |
| topic | green waste <i>Cupriavidus necator</i> α-humulene terpenoid biorefinery bioeconomy |
| url | https://www.mdpi.com/1420-3049/27/24/8684 |
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