High-Risk Human Papillomavirus Detection via Cobas<sup>®</sup> 4800 and REBA HPV-ID<sup>®</sup> Assays
Persistent infection with high-risk human papillomaviruses (HR-HPVs), particularly HPV16 and 18, has long been known to induce cervical cancer progression. However, given that a minority of HPV-infected women develop cancer, analysis of HR-HPV-infected women could help to predict who is at risk of a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/12/2713 |
| _version_ | 1827636588480299008 |
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| author | Sasiprapa Liewchalermwong Shina Oranratanaphan Wichai Termrungruanglert Surang Triratanachat Patou Tantbirojn Nakarin Kitkumthorn Parvapan Bhattarakosol Arkom Chaiwongkot |
| author_facet | Sasiprapa Liewchalermwong Shina Oranratanaphan Wichai Termrungruanglert Surang Triratanachat Patou Tantbirojn Nakarin Kitkumthorn Parvapan Bhattarakosol Arkom Chaiwongkot |
| author_sort | Sasiprapa Liewchalermwong |
| collection | DOAJ |
| description | Persistent infection with high-risk human papillomaviruses (HR-HPVs), particularly HPV16 and 18, has long been known to induce cervical cancer progression. However, given that a minority of HPV-infected women develop cancer, analysis of HR-HPV-infected women could help to predict who is at risk of acquiring cervical cancer. Therefore, to improve HR-HPVs detection, we used the FDA-approved cobas<sup>®</sup> 4800 HPV and REBA HPV-ID<sup>®</sup> HPV assays to detect HR-HPVs in colposcopy-derived cervical cells from 303 patients, detecting 72.28% (219) and 71.62% (217) of HR-HPVs positive cases, with HPV16 detection rates of 35.64% (108) and 30.69% (93), respectively. Of the HPV16-positive cases, cobas<sup>®</sup> 4800 and REBA HPV-ID<sup>®</sup> identified 28.81% (51) and 25.42% (45) of the CIN1 cases, and 55% (33) and 50% (30) of the 60 CIN2/3 cases, respectively. HPV-diagnostic concordance was 82.17% overall (kappa = 0.488), 87.45% for HR-HPVs (kappa = 0.689), and 88.33% for CIN2/3 (kappa = 0.51). The HR-HPVs detection rates of these assays were comparable. Our findings reveal that the FDA-approved HR-HPVs detection assay is appropriate for screening women with HR-HPVs infection, and for predicting increased risk of cervical cancer progression. REBA HPV-ID<sup>®</sup> can be used to detect low risk-HPV types in high-grade cervical lesions that are HR-HPV negative as well as in the distribution of HPV types. |
| first_indexed | 2024-03-09T15:44:56Z |
| format | Article |
| id | doaj.art-953ba827f6594004a71591f6311db3c2 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T15:44:56Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-953ba827f6594004a71591f6311db3c22023-11-24T18:38:20ZengMDPI AGViruses1999-49152022-12-011412271310.3390/v14122713High-Risk Human Papillomavirus Detection via Cobas<sup>®</sup> 4800 and REBA HPV-ID<sup>®</sup> AssaysSasiprapa Liewchalermwong0Shina Oranratanaphan1Wichai Termrungruanglert2Surang Triratanachat3Patou Tantbirojn4Nakarin Kitkumthorn5Parvapan Bhattarakosol6Arkom Chaiwongkot7Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok 10400, ThailandDepartment of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandPersistent infection with high-risk human papillomaviruses (HR-HPVs), particularly HPV16 and 18, has long been known to induce cervical cancer progression. However, given that a minority of HPV-infected women develop cancer, analysis of HR-HPV-infected women could help to predict who is at risk of acquiring cervical cancer. Therefore, to improve HR-HPVs detection, we used the FDA-approved cobas<sup>®</sup> 4800 HPV and REBA HPV-ID<sup>®</sup> HPV assays to detect HR-HPVs in colposcopy-derived cervical cells from 303 patients, detecting 72.28% (219) and 71.62% (217) of HR-HPVs positive cases, with HPV16 detection rates of 35.64% (108) and 30.69% (93), respectively. Of the HPV16-positive cases, cobas<sup>®</sup> 4800 and REBA HPV-ID<sup>®</sup> identified 28.81% (51) and 25.42% (45) of the CIN1 cases, and 55% (33) and 50% (30) of the 60 CIN2/3 cases, respectively. HPV-diagnostic concordance was 82.17% overall (kappa = 0.488), 87.45% for HR-HPVs (kappa = 0.689), and 88.33% for CIN2/3 (kappa = 0.51). The HR-HPVs detection rates of these assays were comparable. Our findings reveal that the FDA-approved HR-HPVs detection assay is appropriate for screening women with HR-HPVs infection, and for predicting increased risk of cervical cancer progression. REBA HPV-ID<sup>®</sup> can be used to detect low risk-HPV types in high-grade cervical lesions that are HR-HPV negative as well as in the distribution of HPV types.https://www.mdpi.com/1999-4915/14/12/2713HPV testingcervical cancer |
| spellingShingle | Sasiprapa Liewchalermwong Shina Oranratanaphan Wichai Termrungruanglert Surang Triratanachat Patou Tantbirojn Nakarin Kitkumthorn Parvapan Bhattarakosol Arkom Chaiwongkot High-Risk Human Papillomavirus Detection via Cobas<sup>®</sup> 4800 and REBA HPV-ID<sup>®</sup> Assays Viruses HPV testing cervical cancer |
| title | High-Risk Human Papillomavirus Detection via Cobas<sup>®</sup> 4800 and REBA HPV-ID<sup>®</sup> Assays |
| title_full | High-Risk Human Papillomavirus Detection via Cobas<sup>®</sup> 4800 and REBA HPV-ID<sup>®</sup> Assays |
| title_fullStr | High-Risk Human Papillomavirus Detection via Cobas<sup>®</sup> 4800 and REBA HPV-ID<sup>®</sup> Assays |
| title_full_unstemmed | High-Risk Human Papillomavirus Detection via Cobas<sup>®</sup> 4800 and REBA HPV-ID<sup>®</sup> Assays |
| title_short | High-Risk Human Papillomavirus Detection via Cobas<sup>®</sup> 4800 and REBA HPV-ID<sup>®</sup> Assays |
| title_sort | high risk human papillomavirus detection via cobas sup r sup 4800 and reba hpv id sup r sup assays |
| topic | HPV testing cervical cancer |
| url | https://www.mdpi.com/1999-4915/14/12/2713 |
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