Synergistic Antifungal Interaction between <i>Pseudomonas aeruginosa</i> LV Strain Metabolites and Biogenic Silver Nanoparticles against <i>Candida auris</i>
<i>Candida auris</i> has been found to be a persistent colonizer of human skin and a successful pathogen capable of causing potentially fatal infection, especially in immunocompromised individuals. This fungal species is usually resistant to most antifungal agents and has the ability to...
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| Format: | Članak |
| Jezik: | English |
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MDPI AG
2023-05-01
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| Serija: | Antibiotics |
| Teme: | |
| Online pristup: | https://www.mdpi.com/2079-6382/12/5/861 |
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| author | Laís Fernanda de Almeida Spoladori Gabriella Maria Andriani Isabela Madeira de Castro Helena Tiemi Suzukawa Ana Carolina Ramos Gimenes Guilherme Bartolomeu-Gonçalves Kelly Ishida Gerson Nakazato Phileno Pinge-Filho Rayanne Regina Beltrame Machado Celso Vataru Nakamura Galdino Andrade Eliandro Reis Tavares Lucy Megumi Yamauchi Sueli Fumie Yamada-Ogatta |
| author_facet | Laís Fernanda de Almeida Spoladori Gabriella Maria Andriani Isabela Madeira de Castro Helena Tiemi Suzukawa Ana Carolina Ramos Gimenes Guilherme Bartolomeu-Gonçalves Kelly Ishida Gerson Nakazato Phileno Pinge-Filho Rayanne Regina Beltrame Machado Celso Vataru Nakamura Galdino Andrade Eliandro Reis Tavares Lucy Megumi Yamauchi Sueli Fumie Yamada-Ogatta |
| author_sort | Laís Fernanda de Almeida Spoladori |
| collection | DOAJ |
| description | <i>Candida auris</i> has been found to be a persistent colonizer of human skin and a successful pathogen capable of causing potentially fatal infection, especially in immunocompromised individuals. This fungal species is usually resistant to most antifungal agents and has the ability to form biofilms on different surfaces, representing a significant therapeutic challenge. Herein, the effect of metabolites of <i>Pseudomonas aeruginosa</i> LV strain, alone and combined with biologically synthesized silver nanoparticles (bioAgNP), was evaluated in planktonic and sessile (biofilm) cells of <i>C. auris</i>. First, the minimal inhibitory and fungicidal concentration values of 3.12 and 6.25 μg/mL, respectively, were determined for F4a, a semi-purified bacterial fraction. Fluopsin C and indolin-3-one seem to be the active components of F4a. Like the semi-purified fraction, they showed a time- and dose-dependent fungicidal activity. F4a and bioAgNP caused severe changes in the morphology and ultrastructure of fungal cells. F4a and indolin-3-one combined with bioAgNP exhibited synergistic fungicidal activity against planktonic cells. F4a, alone or combined with bioAgNP, also caused a significant decrease in the number of viable cells within the biofilms. No cytotoxicity to mammalian cells was detected for bacterial metabolites combined with bioAgNP at synergistic concentrations that presented antifungal activity. These results indicate the potential of F4a combined with bioAgNP as a new strategy for controlling <i>C. auris</i> infections. |
| first_indexed | 2024-03-11T04:00:48Z |
| format | Article |
| id | doaj.art-9540526405034ac1b56ff3e43c94376c |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-11T04:00:48Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-9540526405034ac1b56ff3e43c94376c2023-11-18T00:12:22ZengMDPI AGAntibiotics2079-63822023-05-0112586110.3390/antibiotics12050861Synergistic Antifungal Interaction between <i>Pseudomonas aeruginosa</i> LV Strain Metabolites and Biogenic Silver Nanoparticles against <i>Candida auris</i>Laís Fernanda de Almeida Spoladori0Gabriella Maria Andriani1Isabela Madeira de Castro2Helena Tiemi Suzukawa3Ana Carolina Ramos Gimenes4Guilherme Bartolomeu-Gonçalves5Kelly Ishida6Gerson Nakazato7Phileno Pinge-Filho8Rayanne Regina Beltrame Machado9Celso Vataru Nakamura10Galdino Andrade11Eliandro Reis Tavares12Lucy Megumi Yamauchi13Sueli Fumie Yamada-Ogatta14Programa de Pós-Graduação em Microbiologia, Universidade Estadual de Londrina, Londrina CEP 86057-970, BrazilPrograma de Pós-Graduação em Microbiologia, Universidade Estadual de Londrina, Londrina CEP 86057-970, BrazilPrograma de Pós-Graduação em Microbiologia, Universidade Estadual de Londrina, Londrina CEP 86057-970, BrazilPrograma de Pós-Graduação em Microbiologia, Universidade Estadual de Londrina, Londrina CEP 86057-970, BrazilLaboratório de Biologia Molecular de Microrganismos, Universidade Estadual de Londrina, Londrina CEP 86057-970, BrazilPrograma de Pós-Graduação em Fisiopatologia Clínica e Laboratorial, Universidade Estadual de Londrina, Londrina CEP 86038-350, BrazilLaboratório de Quimioterapia Antifúngica, Universidade de São Paulo, São Paulo CEP 05508-000, BrazilPrograma de Pós-Graduação em Microbiologia, Universidade Estadual de Londrina, Londrina CEP 86057-970, BrazilPrograma de Pós-Graduação em Microbiologia, Universidade Estadual de Londrina, Londrina CEP 86057-970, BrazilLaboratório de Inovação Tecnológica no Desenvolvimento de Fármacos e Cosméticos, Universidade Estadual de Maringá, Maringá CEP 87020-900, BrazilPrograma de Pós-Graduação em Microbiologia, Universidade Estadual de Londrina, Londrina CEP 86057-970, BrazilPrograma de Pós-Graduação em Microbiologia, Universidade Estadual de Londrina, Londrina CEP 86057-970, BrazilLaboratório de Biologia Molecular de Microrganismos, Universidade Estadual de Londrina, Londrina CEP 86057-970, BrazilPrograma de Pós-Graduação em Microbiologia, Universidade Estadual de Londrina, Londrina CEP 86057-970, BrazilPrograma de Pós-Graduação em Microbiologia, Universidade Estadual de Londrina, Londrina CEP 86057-970, Brazil<i>Candida auris</i> has been found to be a persistent colonizer of human skin and a successful pathogen capable of causing potentially fatal infection, especially in immunocompromised individuals. This fungal species is usually resistant to most antifungal agents and has the ability to form biofilms on different surfaces, representing a significant therapeutic challenge. Herein, the effect of metabolites of <i>Pseudomonas aeruginosa</i> LV strain, alone and combined with biologically synthesized silver nanoparticles (bioAgNP), was evaluated in planktonic and sessile (biofilm) cells of <i>C. auris</i>. First, the minimal inhibitory and fungicidal concentration values of 3.12 and 6.25 μg/mL, respectively, were determined for F4a, a semi-purified bacterial fraction. Fluopsin C and indolin-3-one seem to be the active components of F4a. Like the semi-purified fraction, they showed a time- and dose-dependent fungicidal activity. F4a and bioAgNP caused severe changes in the morphology and ultrastructure of fungal cells. F4a and indolin-3-one combined with bioAgNP exhibited synergistic fungicidal activity against planktonic cells. F4a, alone or combined with bioAgNP, also caused a significant decrease in the number of viable cells within the biofilms. No cytotoxicity to mammalian cells was detected for bacterial metabolites combined with bioAgNP at synergistic concentrations that presented antifungal activity. These results indicate the potential of F4a combined with bioAgNP as a new strategy for controlling <i>C. auris</i> infections.https://www.mdpi.com/2079-6382/12/5/861antibiofilm activityantifungal synergismfluopsin Cgreen silver nanoparticlesindolin-3-one |
| spellingShingle | Laís Fernanda de Almeida Spoladori Gabriella Maria Andriani Isabela Madeira de Castro Helena Tiemi Suzukawa Ana Carolina Ramos Gimenes Guilherme Bartolomeu-Gonçalves Kelly Ishida Gerson Nakazato Phileno Pinge-Filho Rayanne Regina Beltrame Machado Celso Vataru Nakamura Galdino Andrade Eliandro Reis Tavares Lucy Megumi Yamauchi Sueli Fumie Yamada-Ogatta Synergistic Antifungal Interaction between <i>Pseudomonas aeruginosa</i> LV Strain Metabolites and Biogenic Silver Nanoparticles against <i>Candida auris</i> Antibiotics antibiofilm activity antifungal synergism fluopsin C green silver nanoparticles indolin-3-one |
| title | Synergistic Antifungal Interaction between <i>Pseudomonas aeruginosa</i> LV Strain Metabolites and Biogenic Silver Nanoparticles against <i>Candida auris</i> |
| title_full | Synergistic Antifungal Interaction between <i>Pseudomonas aeruginosa</i> LV Strain Metabolites and Biogenic Silver Nanoparticles against <i>Candida auris</i> |
| title_fullStr | Synergistic Antifungal Interaction between <i>Pseudomonas aeruginosa</i> LV Strain Metabolites and Biogenic Silver Nanoparticles against <i>Candida auris</i> |
| title_full_unstemmed | Synergistic Antifungal Interaction between <i>Pseudomonas aeruginosa</i> LV Strain Metabolites and Biogenic Silver Nanoparticles against <i>Candida auris</i> |
| title_short | Synergistic Antifungal Interaction between <i>Pseudomonas aeruginosa</i> LV Strain Metabolites and Biogenic Silver Nanoparticles against <i>Candida auris</i> |
| title_sort | synergistic antifungal interaction between i pseudomonas aeruginosa i lv strain metabolites and biogenic silver nanoparticles against i candida auris i |
| topic | antibiofilm activity antifungal synergism fluopsin C green silver nanoparticles indolin-3-one |
| url | https://www.mdpi.com/2079-6382/12/5/861 |
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