Small RNA Profiling of Cucurbit Yellow Stunting Disorder Virus from Susceptible and Tolerant Squash (<i>Cucurbita pepo</i>) Lines
RNA silencing is a crucial mechanism of the antiviral immunity system in plants. Small RNAs guide Argonaut proteins to target viral RNA or DNA, preventing virus accumulation. Small RNA profiles in <i>Cucurbita pepo</i> line PI 420328 with tolerance to cucurbit yellow stunting disorder vi...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-03-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/15/3/788 |
| _version_ | 1797608631998349312 |
|---|---|
| author | Saritha Raman Kavalappara Sudeep Bag Alex Luckew Cecilia E. McGregor |
| author_facet | Saritha Raman Kavalappara Sudeep Bag Alex Luckew Cecilia E. McGregor |
| author_sort | Saritha Raman Kavalappara |
| collection | DOAJ |
| description | RNA silencing is a crucial mechanism of the antiviral immunity system in plants. Small RNAs guide Argonaut proteins to target viral RNA or DNA, preventing virus accumulation. Small RNA profiles in <i>Cucurbita pepo</i> line PI 420328 with tolerance to cucurbit yellow stunting disorder virus (CYSDV) were compared with those in Gold Star, a susceptible cultivar. The lower CYSDV symptom severity in PI 420328 correlated with lower virus titers and fewer sRNAs derived from CYSDV (vsRNA) compared to Gold Star. Elevated levels of 21- and 22-nucleotide (nt) size class vsRNAs were observed in PI 420328, indicating more robust and efficient RNA silencing in PI 420328. The distribution of vsRNA hotspots along the CYSDV genome was similar in both PI 420328 and Gold Star. However, the 3’ UTRs, CPm, and p26 were targeted at a higher frequency in PI 420328. |
| first_indexed | 2024-03-11T05:45:57Z |
| format | Article |
| id | doaj.art-9541e9301e254321b5111ba45bb21a5f |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T05:45:57Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-9541e9301e254321b5111ba45bb21a5f2023-11-17T14:24:21ZengMDPI AGViruses1999-49152023-03-0115378810.3390/v15030788Small RNA Profiling of Cucurbit Yellow Stunting Disorder Virus from Susceptible and Tolerant Squash (<i>Cucurbita pepo</i>) LinesSaritha Raman Kavalappara0Sudeep Bag1Alex Luckew2Cecilia E. McGregor3Department of Plant Pathology, University of Georgia, Tifton, GA 31793, USADepartment of Plant Pathology, University of Georgia, Tifton, GA 31793, USADepartment of Horticulture, University of Georgia, Athens, GA 30602, USADepartment of Horticulture, University of Georgia, Athens, GA 30602, USARNA silencing is a crucial mechanism of the antiviral immunity system in plants. Small RNAs guide Argonaut proteins to target viral RNA or DNA, preventing virus accumulation. Small RNA profiles in <i>Cucurbita pepo</i> line PI 420328 with tolerance to cucurbit yellow stunting disorder virus (CYSDV) were compared with those in Gold Star, a susceptible cultivar. The lower CYSDV symptom severity in PI 420328 correlated with lower virus titers and fewer sRNAs derived from CYSDV (vsRNA) compared to Gold Star. Elevated levels of 21- and 22-nucleotide (nt) size class vsRNAs were observed in PI 420328, indicating more robust and efficient RNA silencing in PI 420328. The distribution of vsRNA hotspots along the CYSDV genome was similar in both PI 420328 and Gold Star. However, the 3’ UTRs, CPm, and p26 were targeted at a higher frequency in PI 420328.https://www.mdpi.com/1999-4915/15/3/788cucurbit yellow stunting disorder virusvirus-derived small RNA<i>Cucurbita pepo</i>tolerancecrinivirus |
| spellingShingle | Saritha Raman Kavalappara Sudeep Bag Alex Luckew Cecilia E. McGregor Small RNA Profiling of Cucurbit Yellow Stunting Disorder Virus from Susceptible and Tolerant Squash (<i>Cucurbita pepo</i>) Lines Viruses cucurbit yellow stunting disorder virus virus-derived small RNA <i>Cucurbita pepo</i> tolerance crinivirus |
| title | Small RNA Profiling of Cucurbit Yellow Stunting Disorder Virus from Susceptible and Tolerant Squash (<i>Cucurbita pepo</i>) Lines |
| title_full | Small RNA Profiling of Cucurbit Yellow Stunting Disorder Virus from Susceptible and Tolerant Squash (<i>Cucurbita pepo</i>) Lines |
| title_fullStr | Small RNA Profiling of Cucurbit Yellow Stunting Disorder Virus from Susceptible and Tolerant Squash (<i>Cucurbita pepo</i>) Lines |
| title_full_unstemmed | Small RNA Profiling of Cucurbit Yellow Stunting Disorder Virus from Susceptible and Tolerant Squash (<i>Cucurbita pepo</i>) Lines |
| title_short | Small RNA Profiling of Cucurbit Yellow Stunting Disorder Virus from Susceptible and Tolerant Squash (<i>Cucurbita pepo</i>) Lines |
| title_sort | small rna profiling of cucurbit yellow stunting disorder virus from susceptible and tolerant squash i cucurbita pepo i lines |
| topic | cucurbit yellow stunting disorder virus virus-derived small RNA <i>Cucurbita pepo</i> tolerance crinivirus |
| url | https://www.mdpi.com/1999-4915/15/3/788 |
| work_keys_str_mv | AT saritharamankavalappara smallrnaprofilingofcucurbityellowstuntingdisordervirusfromsusceptibleandtolerantsquashicucurbitapepoilines AT sudeepbag smallrnaprofilingofcucurbityellowstuntingdisordervirusfromsusceptibleandtolerantsquashicucurbitapepoilines AT alexluckew smallrnaprofilingofcucurbityellowstuntingdisordervirusfromsusceptibleandtolerantsquashicucurbitapepoilines AT ceciliaemcgregor smallrnaprofilingofcucurbityellowstuntingdisordervirusfromsusceptibleandtolerantsquashicucurbitapepoilines |