The effects of aqueous extract of ocimum gratissimum on the cerebellum of male wistar rats challenged by lead acetate
Summary: Objectives: Lead acetate (LA) is a known toxicant, and its exposure in the environment has been on the increase in recent times, leading to oxidative stress and tissue damage. Based on this background, we investigated the role of the antioxidative properties of Ocimum gratissimum (OG), a p...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Clinical Nutrition Open Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667268522000304 |
| _version_ | 1811215368209825792 |
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| author | Onoriode Andrew Udi John Chukwuma Oyem Oladuni Abimbola Ebeye Lilian Ebite Chris-Ozoko Patrick Sunday Igbigbi Donald Uzowulu Olannye |
| author_facet | Onoriode Andrew Udi John Chukwuma Oyem Oladuni Abimbola Ebeye Lilian Ebite Chris-Ozoko Patrick Sunday Igbigbi Donald Uzowulu Olannye |
| author_sort | Onoriode Andrew Udi |
| collection | DOAJ |
| description | Summary: Objectives: Lead acetate (LA) is a known toxicant, and its exposure in the environment has been on the increase in recent times, leading to oxidative stress and tissue damage. Based on this background, we investigated the role of the antioxidative properties of Ocimum gratissimum (OG), a potent medicinal plant, in ameliorating and protecting the brain from lead acetate-induced cerebellar damage via the assessment of oxidative stress parameters and brain histology. Methods: Thirty-five adult male Wistar rats weighing 145–200g divided into five groups were used for this experimental study. Groups 1, 2, and 3 served as positive and negative controls and were administered sterile water, 252.98 mg/kg body weight of aqueous leaves extract of OG and 120 mg/kg of LA for 21 days. Furthermore, following the administration of LA, rats in groups 4 and 5 were treated with 125 and 250 mg/kg body weight of OG. OG and LA were administered orally for 42 days. Oxidative stress parameters, as well as histoarchitectural investigations following LA and OG treatment, were analysed. Results: Results showed normal histoarchitecture of the cerebellum in control and group 2 animals (OG only). In group 3 animals that received LA only, we observed severe stages of neurodegenerative features in the Purkinje, granular cell molecular cell layers of the Cerebellum. Also, results from groups 4 and 5 rats that were co-administered with LA and OG revealed regenerative tendencies and an increase in the neuronal cell density of the cerebellum. Nevertheless, we recorded an increase in the brain content of malondialdehyde and decreased antioxidant status (catalase (CAT), glutathione (GSH), and sodium dismutase (SOD)) following LA administration. OG attenuated this heightened MDA activity and depletion of the antioxidant status. Conclusion: Overall, these findings suggest that oral administration of aqueous extract of OG improved cerebellar neurotoxicity and degeneration in rats exposed to LA by modulating oxidative damage. |
| first_indexed | 2024-04-12T06:20:40Z |
| format | Article |
| id | doaj.art-95428e387c5d49cf9662bbcd4fed40a9 |
| institution | Directory Open Access Journal |
| issn | 2667-2685 |
| language | English |
| last_indexed | 2024-04-12T06:20:40Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Clinical Nutrition Open Science |
| spelling | doaj.art-95428e387c5d49cf9662bbcd4fed40a92022-12-22T03:44:19ZengElsevierClinical Nutrition Open Science2667-26852022-08-01442841The effects of aqueous extract of ocimum gratissimum on the cerebellum of male wistar rats challenged by lead acetateOnoriode Andrew Udi0John Chukwuma Oyem1Oladuni Abimbola Ebeye2Lilian Ebite Chris-Ozoko3Patrick Sunday Igbigbi4Donald Uzowulu Olannye5Department of Human Anatomy Department, Achievers University Owo, Ondo State, NigeriaDepartment of Human Anatomy, Novena University Ogume, Delta State, Nigeria; Department of Psychology, The Arctic University of Norway, Tromsø, Norway; Corresponding author. Department of Psychology, The Arctic University of Norway, 9037, Tromsø, Norway.Departmen of Human Anatomy, Delta State University, Abraka, NigeriaDepartmen of Human Anatomy, Delta State University, Abraka, NigeriaDepartmen of Human Anatomy, Delta State University, Abraka, NigeriaDepartment of Environmental Health Sciences, University of Ibadan, NigeriaSummary: Objectives: Lead acetate (LA) is a known toxicant, and its exposure in the environment has been on the increase in recent times, leading to oxidative stress and tissue damage. Based on this background, we investigated the role of the antioxidative properties of Ocimum gratissimum (OG), a potent medicinal plant, in ameliorating and protecting the brain from lead acetate-induced cerebellar damage via the assessment of oxidative stress parameters and brain histology. Methods: Thirty-five adult male Wistar rats weighing 145–200g divided into five groups were used for this experimental study. Groups 1, 2, and 3 served as positive and negative controls and were administered sterile water, 252.98 mg/kg body weight of aqueous leaves extract of OG and 120 mg/kg of LA for 21 days. Furthermore, following the administration of LA, rats in groups 4 and 5 were treated with 125 and 250 mg/kg body weight of OG. OG and LA were administered orally for 42 days. Oxidative stress parameters, as well as histoarchitectural investigations following LA and OG treatment, were analysed. Results: Results showed normal histoarchitecture of the cerebellum in control and group 2 animals (OG only). In group 3 animals that received LA only, we observed severe stages of neurodegenerative features in the Purkinje, granular cell molecular cell layers of the Cerebellum. Also, results from groups 4 and 5 rats that were co-administered with LA and OG revealed regenerative tendencies and an increase in the neuronal cell density of the cerebellum. Nevertheless, we recorded an increase in the brain content of malondialdehyde and decreased antioxidant status (catalase (CAT), glutathione (GSH), and sodium dismutase (SOD)) following LA administration. OG attenuated this heightened MDA activity and depletion of the antioxidant status. Conclusion: Overall, these findings suggest that oral administration of aqueous extract of OG improved cerebellar neurotoxicity and degeneration in rats exposed to LA by modulating oxidative damage.http://www.sciencedirect.com/science/article/pii/S2667268522000304Ocimum gratissimumCerebellumLead acetateOxidative stressNeurotoxicity |
| spellingShingle | Onoriode Andrew Udi John Chukwuma Oyem Oladuni Abimbola Ebeye Lilian Ebite Chris-Ozoko Patrick Sunday Igbigbi Donald Uzowulu Olannye The effects of aqueous extract of ocimum gratissimum on the cerebellum of male wistar rats challenged by lead acetate Clinical Nutrition Open Science Ocimum gratissimum Cerebellum Lead acetate Oxidative stress Neurotoxicity |
| title | The effects of aqueous extract of ocimum gratissimum on the cerebellum of male wistar rats challenged by lead acetate |
| title_full | The effects of aqueous extract of ocimum gratissimum on the cerebellum of male wistar rats challenged by lead acetate |
| title_fullStr | The effects of aqueous extract of ocimum gratissimum on the cerebellum of male wistar rats challenged by lead acetate |
| title_full_unstemmed | The effects of aqueous extract of ocimum gratissimum on the cerebellum of male wistar rats challenged by lead acetate |
| title_short | The effects of aqueous extract of ocimum gratissimum on the cerebellum of male wistar rats challenged by lead acetate |
| title_sort | effects of aqueous extract of ocimum gratissimum on the cerebellum of male wistar rats challenged by lead acetate |
| topic | Ocimum gratissimum Cerebellum Lead acetate Oxidative stress Neurotoxicity |
| url | http://www.sciencedirect.com/science/article/pii/S2667268522000304 |
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