Characterization of Perinatal Stem Cell Spheroids for the Development of Cell Therapy Strategy
Type 1 diabetes mellitus (T1DM) is a complex metabolic disease characterized by a massive loss of insulin-producing cells due to an autoimmune reaction. Currently, daily subcutaneous administration of exogenous insulin is the only effective treatment. Therefore, in recent years considerable interest...
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MDPI AG
2023-02-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/10/2/189 |
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| author | Francesca Paris Pasquale Marrazzo Valeria Pizzuti Cosetta Marchionni Maura Rossi Martina Michelotti Biljana Petrovic Elisabetta Ciani Giuliana Simonazzi Andrea Pession Laura Bonsi Francesco Alviano |
| author_facet | Francesca Paris Pasquale Marrazzo Valeria Pizzuti Cosetta Marchionni Maura Rossi Martina Michelotti Biljana Petrovic Elisabetta Ciani Giuliana Simonazzi Andrea Pession Laura Bonsi Francesco Alviano |
| author_sort | Francesca Paris |
| collection | DOAJ |
| description | Type 1 diabetes mellitus (T1DM) is a complex metabolic disease characterized by a massive loss of insulin-producing cells due to an autoimmune reaction. Currently, daily subcutaneous administration of exogenous insulin is the only effective treatment. Therefore, in recent years considerable interest has been given to stem cell therapy and in particular to the use of three-dimensional (3D) cell cultures to better reproduce in vivo conditions. The goal of this study is to provide a reliable cellular model that could be investigated for regenerative medicine applications for the replacement of insulin-producing cells in T1DM. To pursue this aim we create a co-culture spheroid of amniotic epithelial cells (AECs) and Wharton’s jelly mesenchymal stromal cells (WJ-MSCs) in a one-to-one ratio. The resulting co-culture spheroids were analyzed for viability, extracellular matrix production, and hypoxic state in both early- and long-term cultures. Our results suggest that co-culture spheroids are stable in long-term culture and are still viable with a consistent extracellular matrix production evaluated with immunofluorescence staining. These findings suggest that this co-culture may potentially be differentiated into endo-pancreatic cells for regenerative medicine applications in T1DM. |
| first_indexed | 2024-03-11T09:09:11Z |
| format | Article |
| id | doaj.art-9545c82f0e7e492caa4182b09645a872 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-11T09:09:11Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-9545c82f0e7e492caa4182b09645a8722023-11-16T19:10:53ZengMDPI AGBioengineering2306-53542023-02-0110218910.3390/bioengineering10020189Characterization of Perinatal Stem Cell Spheroids for the Development of Cell Therapy StrategyFrancesca Paris0Pasquale Marrazzo1Valeria Pizzuti2Cosetta Marchionni3Maura Rossi4Martina Michelotti5Biljana Petrovic6Elisabetta Ciani7Giuliana Simonazzi8Andrea Pession9Laura Bonsi10Francesco Alviano11Unit of Histology, Embryology and Applied Biology, Department of Medical and Surgical Sciences, University of Bologna, 40126 Bologna, ItalyUnit of Histology, Embryology and Applied Biology, Department of Medical and Surgical Sciences, University of Bologna, 40126 Bologna, ItalyUnit of Histology, Embryology and Applied Biology, Department of Medical and Surgical Sciences, University of Bologna, 40126 Bologna, ItalyUnit of Histology, Embryology and Applied Biology, Department of Medical and Surgical Sciences, University of Bologna, 40126 Bologna, ItalyDepartment of Medical and Surgical Sciences, University of Bologna, 40138 Bologna, ItalyUnit of Histology, Embryology and Applied Biology, Department of Medical and Surgical Sciences, University of Bologna, 40126 Bologna, ItalyDepartment of Medical and Surgical Sciences, University of Bologna, 40138 Bologna, ItalyDepartment of Biomedical and Neuromotor Science, University of Bologna, 40126 Bologna, ItalyDepartment of Medical and Surgical Sciences, University of Bologna, 40138 Bologna, ItalyPediatric Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, ItalyUnit of Histology, Embryology and Applied Biology, Department of Medical and Surgical Sciences, University of Bologna, 40126 Bologna, ItalyUnit of Histology, Embryology and Applied Biology, Department of Medical and Surgical Sciences, University of Bologna, 40126 Bologna, ItalyType 1 diabetes mellitus (T1DM) is a complex metabolic disease characterized by a massive loss of insulin-producing cells due to an autoimmune reaction. Currently, daily subcutaneous administration of exogenous insulin is the only effective treatment. Therefore, in recent years considerable interest has been given to stem cell therapy and in particular to the use of three-dimensional (3D) cell cultures to better reproduce in vivo conditions. The goal of this study is to provide a reliable cellular model that could be investigated for regenerative medicine applications for the replacement of insulin-producing cells in T1DM. To pursue this aim we create a co-culture spheroid of amniotic epithelial cells (AECs) and Wharton’s jelly mesenchymal stromal cells (WJ-MSCs) in a one-to-one ratio. The resulting co-culture spheroids were analyzed for viability, extracellular matrix production, and hypoxic state in both early- and long-term cultures. Our results suggest that co-culture spheroids are stable in long-term culture and are still viable with a consistent extracellular matrix production evaluated with immunofluorescence staining. These findings suggest that this co-culture may potentially be differentiated into endo-pancreatic cells for regenerative medicine applications in T1DM.https://www.mdpi.com/2306-5354/10/2/189type 1 diabetesamniotic membraneumbilical cordperinatal cellsplacenta stem cellsamniotic epithelial cells |
| spellingShingle | Francesca Paris Pasquale Marrazzo Valeria Pizzuti Cosetta Marchionni Maura Rossi Martina Michelotti Biljana Petrovic Elisabetta Ciani Giuliana Simonazzi Andrea Pession Laura Bonsi Francesco Alviano Characterization of Perinatal Stem Cell Spheroids for the Development of Cell Therapy Strategy Bioengineering type 1 diabetes amniotic membrane umbilical cord perinatal cells placenta stem cells amniotic epithelial cells |
| title | Characterization of Perinatal Stem Cell Spheroids for the Development of Cell Therapy Strategy |
| title_full | Characterization of Perinatal Stem Cell Spheroids for the Development of Cell Therapy Strategy |
| title_fullStr | Characterization of Perinatal Stem Cell Spheroids for the Development of Cell Therapy Strategy |
| title_full_unstemmed | Characterization of Perinatal Stem Cell Spheroids for the Development of Cell Therapy Strategy |
| title_short | Characterization of Perinatal Stem Cell Spheroids for the Development of Cell Therapy Strategy |
| title_sort | characterization of perinatal stem cell spheroids for the development of cell therapy strategy |
| topic | type 1 diabetes amniotic membrane umbilical cord perinatal cells placenta stem cells amniotic epithelial cells |
| url | https://www.mdpi.com/2306-5354/10/2/189 |
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