Caveolin-1-dependent and -independent membrane domains[S]
Lipid rafts defined as cholesterol- and sphingomyelin-rich domains have been isolated from different cell types that vary greatly in their lipid profiles. Here, we investigated the contribution of the structural protein caveolin-1 (Cav1) to the overall lipid composition and domain abundance in mouse...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2009-08-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520307628 |
| _version_ | 1818601001977905152 |
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| author | Soazig Le Lay Qiong Li Nicholas Proschogo Macarena Rodriguez Krishanthi Gunaratnam Siân Cartland Carles Rentero Wendy Jessup Todd Mitchell Katharina Gaus |
| author_facet | Soazig Le Lay Qiong Li Nicholas Proschogo Macarena Rodriguez Krishanthi Gunaratnam Siân Cartland Carles Rentero Wendy Jessup Todd Mitchell Katharina Gaus |
| author_sort | Soazig Le Lay |
| collection | DOAJ |
| description | Lipid rafts defined as cholesterol- and sphingomyelin-rich domains have been isolated from different cell types that vary greatly in their lipid profiles. Here, we investigated the contribution of the structural protein caveolin-1 (Cav1) to the overall lipid composition and domain abundance in mouse embryonic fibroblasts (MEFs) from wild-type (WT) or Cav1-deficient (Cav1−/−) animals. Our findings show that Cav1 expression had no effect on free (membrane-associated) cholesterol levels. However, Cav1−/−-deficient cells did have a higher proportion of sphingomyelin, decreased abundance of unsaturated phospholipids, and a trend toward shorter fatty acid chains in phosphatidylcholine. We isolated detergent-resistant membranes (DRMs), nondetergent raft domains (NDR), and cholesterol oxidase (CO)-sensitive domains and assessed the abundance of ordered domains in intact cells using the fluorescent dye Laurdan. Despite differences in phospholipid composition, we found that cholesterol levels in DRMs, NDR, and CO-sensitive domains were similar in both cell types. The data suggest that Cav1 is not required to target cholesterol to lipid rafts and that CO does not specifically oxidize caveolar cholesterol. In contrast, the abundance of ordered domains in adherent cells is reduced in Cav1−/− compared with WT MEFs, suggesting that cell architecture is critical in maintaining Cav1-induced lipid rafts. |
| first_indexed | 2024-12-16T12:44:27Z |
| format | Article |
| id | doaj.art-95462ec900054466a8a743b31ceddce6 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-16T12:44:27Z |
| publishDate | 2009-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-95462ec900054466a8a743b31ceddce62022-12-21T22:31:19ZengElsevierJournal of Lipid Research0022-22752009-08-0150816091620Caveolin-1-dependent and -independent membrane domains[S]Soazig Le Lay0Qiong Li1Nicholas Proschogo2Macarena Rodriguez3Krishanthi Gunaratnam4Siân Cartland5Carles Rentero6Wendy Jessup7Todd Mitchell8Katharina Gaus9Centre de Recherche des Cordeliers, INSERM, U872, Université Pierre et Marie Curie, Paris 6, France; Université Paris Descartes, Paris F-75006, FranceCentre for Vascular Research, University of New South Wales; Department of Haematology, Prince of Wales Hospital, Sydney, AustraliaCentre for Vascular Research, University of New South Wales; Department of Haematology, Prince of Wales Hospital, Sydney, AustraliaCentre for Vascular Research, University of New South Wales; Department of Haematology, Prince of Wales Hospital, Sydney, AustraliaCentre for Vascular Research, University of New South Wales; Department of Haematology, Prince of Wales Hospital, Sydney, AustraliaCentre for Vascular Research, University of New South Wales; Department of Haematology, Prince of Wales Hospital, Sydney, AustraliaCentre for Vascular Research, University of New South Wales; Department of Haematology, Prince of Wales Hospital, Sydney, AustraliaCentre for Vascular Research, University of New South Wales; Department of Haematology, Prince of Wales Hospital, Sydney, AustraliaCentre for Vascular Research, University of New South Wales; Department of Haematology, Prince of Wales Hospital, Sydney, Australia; School of Health Sciences, University of Wollongong, Wollongong, AustraliaTo whom correspondence should be addressed.; Centre for Vascular Research, University of New South Wales; Department of Haematology, Prince of Wales Hospital, Sydney, AustraliaLipid rafts defined as cholesterol- and sphingomyelin-rich domains have been isolated from different cell types that vary greatly in their lipid profiles. Here, we investigated the contribution of the structural protein caveolin-1 (Cav1) to the overall lipid composition and domain abundance in mouse embryonic fibroblasts (MEFs) from wild-type (WT) or Cav1-deficient (Cav1−/−) animals. Our findings show that Cav1 expression had no effect on free (membrane-associated) cholesterol levels. However, Cav1−/−-deficient cells did have a higher proportion of sphingomyelin, decreased abundance of unsaturated phospholipids, and a trend toward shorter fatty acid chains in phosphatidylcholine. We isolated detergent-resistant membranes (DRMs), nondetergent raft domains (NDR), and cholesterol oxidase (CO)-sensitive domains and assessed the abundance of ordered domains in intact cells using the fluorescent dye Laurdan. Despite differences in phospholipid composition, we found that cholesterol levels in DRMs, NDR, and CO-sensitive domains were similar in both cell types. The data suggest that Cav1 is not required to target cholesterol to lipid rafts and that CO does not specifically oxidize caveolar cholesterol. In contrast, the abundance of ordered domains in adherent cells is reduced in Cav1−/− compared with WT MEFs, suggesting that cell architecture is critical in maintaining Cav1-induced lipid rafts.http://www.sciencedirect.com/science/article/pii/S0022227520307628lipid raftscaveolaelipid profile |
| spellingShingle | Soazig Le Lay Qiong Li Nicholas Proschogo Macarena Rodriguez Krishanthi Gunaratnam Siân Cartland Carles Rentero Wendy Jessup Todd Mitchell Katharina Gaus Caveolin-1-dependent and -independent membrane domains[S] Journal of Lipid Research lipid rafts caveolae lipid profile |
| title | Caveolin-1-dependent and -independent membrane domains[S] |
| title_full | Caveolin-1-dependent and -independent membrane domains[S] |
| title_fullStr | Caveolin-1-dependent and -independent membrane domains[S] |
| title_full_unstemmed | Caveolin-1-dependent and -independent membrane domains[S] |
| title_short | Caveolin-1-dependent and -independent membrane domains[S] |
| title_sort | caveolin 1 dependent and independent membrane domains s |
| topic | lipid rafts caveolae lipid profile |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520307628 |
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