Construction of an ATP-Activated Y-Shape DNA Probe for Smart miRNA Imaging in Living Cells
A stringent DNA probe to profile microRNA (miRNA) expression within a specific cell remains a key challenge in biology. To address this issue, an intracellular ATP-activated Y-DNA probe for accurate imaging of miRNA in living cells was designed. Y-DNA was based on the fabrication of tripartite funct...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Chemistry |
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| Online Access: | https://www.mdpi.com/2624-8549/5/3/112 |
| _version_ | 1797580819364052992 |
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| author | Wukun Zhong Yanlin Zheng Lei Huang Chao Xing Chunhua Lu |
| author_facet | Wukun Zhong Yanlin Zheng Lei Huang Chao Xing Chunhua Lu |
| author_sort | Wukun Zhong |
| collection | DOAJ |
| description | A stringent DNA probe to profile microRNA (miRNA) expression within a specific cell remains a key challenge in biology. To address this issue, an intracellular ATP-activated Y-DNA probe for accurate imaging of miRNA in living cells was designed. Y-DNA was based on the fabrication of tripartite function modules, which consisted of a folate (FA)-modified targeting module, an ATP aptamer-sealed driver, and a miRNA sensing module. The Y-DNA probe could be specifically activated by ATP after it efficiently internalized into FA-receptor-overexpressed cells based on caveolar-mediated endocytosis, leading to the activation of the miRNA sensing module. The activated Y-DNA probe allowed for the imaging of miRNA in living cells with high sensitivity. The design of the ATP-activated Y-DNA sensor opens the door for bioorthogonal miRNA imaging and promotes the development of various responsive DNA molecular probes with enhanced anti-interference ability for clinical diagnosis. |
| first_indexed | 2024-03-10T22:55:25Z |
| format | Article |
| id | doaj.art-9546538217a7477da3c5b44009097bc4 |
| institution | Directory Open Access Journal |
| issn | 2624-8549 |
| language | English |
| last_indexed | 2024-03-10T22:55:25Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemistry |
| spelling | doaj.art-9546538217a7477da3c5b44009097bc42023-11-19T10:01:22ZengMDPI AGChemistry2624-85492023-07-01531634164410.3390/chemistry5030112Construction of an ATP-Activated Y-Shape DNA Probe for Smart miRNA Imaging in Living CellsWukun Zhong0Yanlin Zheng1Lei Huang2Chao Xing3Chunhua Lu4MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou 350116, ChinaMOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou 350116, ChinaMOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou 350116, ChinaFujian Key Laboratory of Functional Marine Sensing Materials, Center for Advanced Marine Materials and Smart Sensors, College of Materials and Chemical Engineering, Minjiang University, Fuzhou 350108, ChinaMOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou 350116, ChinaA stringent DNA probe to profile microRNA (miRNA) expression within a specific cell remains a key challenge in biology. To address this issue, an intracellular ATP-activated Y-DNA probe for accurate imaging of miRNA in living cells was designed. Y-DNA was based on the fabrication of tripartite function modules, which consisted of a folate (FA)-modified targeting module, an ATP aptamer-sealed driver, and a miRNA sensing module. The Y-DNA probe could be specifically activated by ATP after it efficiently internalized into FA-receptor-overexpressed cells based on caveolar-mediated endocytosis, leading to the activation of the miRNA sensing module. The activated Y-DNA probe allowed for the imaging of miRNA in living cells with high sensitivity. The design of the ATP-activated Y-DNA sensor opens the door for bioorthogonal miRNA imaging and promotes the development of various responsive DNA molecular probes with enhanced anti-interference ability for clinical diagnosis.https://www.mdpi.com/2624-8549/5/3/112fluorescencemicroRNAATPDNA probesimaging |
| spellingShingle | Wukun Zhong Yanlin Zheng Lei Huang Chao Xing Chunhua Lu Construction of an ATP-Activated Y-Shape DNA Probe for Smart miRNA Imaging in Living Cells Chemistry fluorescence microRNA ATP DNA probes imaging |
| title | Construction of an ATP-Activated Y-Shape DNA Probe for Smart miRNA Imaging in Living Cells |
| title_full | Construction of an ATP-Activated Y-Shape DNA Probe for Smart miRNA Imaging in Living Cells |
| title_fullStr | Construction of an ATP-Activated Y-Shape DNA Probe for Smart miRNA Imaging in Living Cells |
| title_full_unstemmed | Construction of an ATP-Activated Y-Shape DNA Probe for Smart miRNA Imaging in Living Cells |
| title_short | Construction of an ATP-Activated Y-Shape DNA Probe for Smart miRNA Imaging in Living Cells |
| title_sort | construction of an atp activated y shape dna probe for smart mirna imaging in living cells |
| topic | fluorescence microRNA ATP DNA probes imaging |
| url | https://www.mdpi.com/2624-8549/5/3/112 |
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