Key genes and regulatory networks of hypoxic preconditioning on osteoblasts
The purpose of this study was to identify the key genes and pathways involved in hypoxic preconditioning of osteoblasts. Cells of the hypoxic-preconditioning group underwent 10 min of hypoxic cultivation/10 min normoxic cultivation three times before 4 h of hypoxic cultivation. The long-time hypoxia...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | All Life |
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| Online Access: | http://dx.doi.org/10.1080/26895293.2023.2169362 |
| _version_ | 1826978979385442304 |
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| author | Dong Wang Xueqiang Wu Xiaobin Zhou Junlin Zhou |
| author_facet | Dong Wang Xueqiang Wu Xiaobin Zhou Junlin Zhou |
| author_sort | Dong Wang |
| collection | DOAJ |
| description | The purpose of this study was to identify the key genes and pathways involved in hypoxic preconditioning of osteoblasts. Cells of the hypoxic-preconditioning group underwent 10 min of hypoxic cultivation/10 min normoxic cultivation three times before 4 h of hypoxic cultivation. The long-time hypoxia group underwent 4 h of hypoxic cultivation, and the control group cells underwent 4 h of normoxic cultivation. Differences in miRNAs expressed were analyzed by high-throughput sequencing. MiRwalk 3.0 was used to predict the target genes and conduct a Gene Set Enrichment Analysis. The STRING database and Cytoscape were used to explore the hub genes. A total of 8635 genes and 121 pathways are enriched in the hypoxic-preconditioning compared with the long-time hypoxia group, including the hub genes Utp6, Eif3a, Etf1, Rrp12 and Mkks. 8,476 target genes and 121 pathways are enriched in the hypoxic-preconditioning group compared with the control group, including the hub genes Chd7, Cebpa and Fosb, and 9,315 target genes and 131 pathways are enriched in the long-term hypoxia group compared with the control group, including the hub genes Dcun1d2, Ube2d1 and Frk. Hypoxia preconditioning improved cell viability (p < 0.01), enhanced ALP, IL10 and VEGF expression, and inhibited IL6 expression (p < 0.05). Hypoxic preconditioning is an effective method for promoting osteoblast activity. |
| first_indexed | 2024-03-09T02:08:58Z |
| format | Article |
| id | doaj.art-9546675a406c4381a27e52bad0bab5dd |
| institution | Directory Open Access Journal |
| issn | 2689-5307 |
| language | English |
| last_indexed | 2025-02-18T05:21:49Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | All Life |
| spelling | doaj.art-9546675a406c4381a27e52bad0bab5dd2024-11-15T10:36:01ZengTaylor & Francis GroupAll Life2689-53072023-12-01160110.1080/26895293.2023.21693622169362Key genes and regulatory networks of hypoxic preconditioning on osteoblastsDong Wang0Xueqiang Wu1Xiaobin Zhou2Junlin Zhou3Beijing Chaoyang hospital, Capital Medical UniversityBeijing Chaoyang hospital, Capital Medical UniversityThe Third Hospital of ShijiazhuangBeijing Chaoyang hospital, Capital Medical UniversityThe purpose of this study was to identify the key genes and pathways involved in hypoxic preconditioning of osteoblasts. Cells of the hypoxic-preconditioning group underwent 10 min of hypoxic cultivation/10 min normoxic cultivation three times before 4 h of hypoxic cultivation. The long-time hypoxia group underwent 4 h of hypoxic cultivation, and the control group cells underwent 4 h of normoxic cultivation. Differences in miRNAs expressed were analyzed by high-throughput sequencing. MiRwalk 3.0 was used to predict the target genes and conduct a Gene Set Enrichment Analysis. The STRING database and Cytoscape were used to explore the hub genes. A total of 8635 genes and 121 pathways are enriched in the hypoxic-preconditioning compared with the long-time hypoxia group, including the hub genes Utp6, Eif3a, Etf1, Rrp12 and Mkks. 8,476 target genes and 121 pathways are enriched in the hypoxic-preconditioning group compared with the control group, including the hub genes Chd7, Cebpa and Fosb, and 9,315 target genes and 131 pathways are enriched in the long-term hypoxia group compared with the control group, including the hub genes Dcun1d2, Ube2d1 and Frk. Hypoxia preconditioning improved cell viability (p < 0.01), enhanced ALP, IL10 and VEGF expression, and inhibited IL6 expression (p < 0.05). Hypoxic preconditioning is an effective method for promoting osteoblast activity.http://dx.doi.org/10.1080/26895293.2023.2169362hypoxic preconditioningosteoblastsregulatory networkhub genes |
| spellingShingle | Dong Wang Xueqiang Wu Xiaobin Zhou Junlin Zhou Key genes and regulatory networks of hypoxic preconditioning on osteoblasts All Life hypoxic preconditioning osteoblasts regulatory network hub genes |
| title | Key genes and regulatory networks of hypoxic preconditioning on osteoblasts |
| title_full | Key genes and regulatory networks of hypoxic preconditioning on osteoblasts |
| title_fullStr | Key genes and regulatory networks of hypoxic preconditioning on osteoblasts |
| title_full_unstemmed | Key genes and regulatory networks of hypoxic preconditioning on osteoblasts |
| title_short | Key genes and regulatory networks of hypoxic preconditioning on osteoblasts |
| title_sort | key genes and regulatory networks of hypoxic preconditioning on osteoblasts |
| topic | hypoxic preconditioning osteoblasts regulatory network hub genes |
| url | http://dx.doi.org/10.1080/26895293.2023.2169362 |
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