Role of Different Pre-Treatments on Composition and Rheology of Synovial Fluids
Different pre-treatments applied to synovial fluids (SF) before their analyses are tested to characterize SF after storage under different conditions and to investigate their evolution along a viscosupplementation treatment. The main techniques proposed involve steric exclusion chromatography with t...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2009-12-01
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| Series: | Polymers |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2073-4360/1/1/16/ |
| _version_ | 1811244462260617216 |
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| author | Pierre Mathieu Thierry Conrozier Yves Rozand Marguerite Rinaudo |
| author_facet | Pierre Mathieu Thierry Conrozier Yves Rozand Marguerite Rinaudo |
| author_sort | Pierre Mathieu |
| collection | DOAJ |
| description | Different pre-treatments applied to synovial fluids (SF) before their analyses are tested to characterize SF after storage under different conditions and to investigate their evolution along a viscosupplementation treatment. The main techniques proposed involve steric exclusion chromatography with triple detection (SEC) and viscometry; it is the first time that such a study is developed. SEC gives the molecular weight distribution and concentration of hyaluronan (HA) and proteins separately; the steady state viscosity is always non-Newtonian and not directly related to SF composition. Pre-treatment of SF (storage in cold, filtration, centrifugation) allows us to conclude that, in order to store SF, it is best to freeze it, even if in some cases, viscosity is modified but not the composition. All the data obtained (including protease pre-treatment) allow us to conclude that a small fraction of HA-protein complex forms a loose 3D-network and controls the rheology. |
| first_indexed | 2024-04-12T14:25:27Z |
| format | Article |
| id | doaj.art-95468ed5c91c4dac86c2cdd8f744872b |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-04-12T14:25:27Z |
| publishDate | 2009-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-95468ed5c91c4dac86c2cdd8f744872b2022-12-22T03:29:28ZengMDPI AGPolymers2073-43602009-12-0111163410.3390/polym1010016Role of Different Pre-Treatments on Composition and Rheology of Synovial FluidsPierre MathieuThierry ConrozierYves RozandMarguerite RinaudoDifferent pre-treatments applied to synovial fluids (SF) before their analyses are tested to characterize SF after storage under different conditions and to investigate their evolution along a viscosupplementation treatment. The main techniques proposed involve steric exclusion chromatography with triple detection (SEC) and viscometry; it is the first time that such a study is developed. SEC gives the molecular weight distribution and concentration of hyaluronan (HA) and proteins separately; the steady state viscosity is always non-Newtonian and not directly related to SF composition. Pre-treatment of SF (storage in cold, filtration, centrifugation) allows us to conclude that, in order to store SF, it is best to freeze it, even if in some cases, viscosity is modified but not the composition. All the data obtained (including protease pre-treatment) allow us to conclude that a small fraction of HA-protein complex forms a loose 3D-network and controls the rheology.http://www.mdpi.com/2073-4360/1/1/16/hyaluronansteric exclusion chromatographyrheologycentrifugationfreezingfiltrationprotease treatment |
| spellingShingle | Pierre Mathieu Thierry Conrozier Yves Rozand Marguerite Rinaudo Role of Different Pre-Treatments on Composition and Rheology of Synovial Fluids Polymers hyaluronan steric exclusion chromatography rheology centrifugation freezing filtration protease treatment |
| title | Role of Different Pre-Treatments on Composition and Rheology of Synovial Fluids |
| title_full | Role of Different Pre-Treatments on Composition and Rheology of Synovial Fluids |
| title_fullStr | Role of Different Pre-Treatments on Composition and Rheology of Synovial Fluids |
| title_full_unstemmed | Role of Different Pre-Treatments on Composition and Rheology of Synovial Fluids |
| title_short | Role of Different Pre-Treatments on Composition and Rheology of Synovial Fluids |
| title_sort | role of different pre treatments on composition and rheology of synovial fluids |
| topic | hyaluronan steric exclusion chromatography rheology centrifugation freezing filtration protease treatment |
| url | http://www.mdpi.com/2073-4360/1/1/16/ |
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